K03328

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)
T2
	whole plant (PO:0000003)	increased height (PATO:0000570) (Adult plant::Plant height::tall)
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small)
	rosette leaf (PO:0000014)	increased size (PATO:0000586) (Rosette leaf::Shape::large) dark green (PATO:0001249) (Rosette leaf::Color::dark) desaturated green (PATO:0001272) (Rosette leaf::Color::pale) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K03328

Determination of introduced cDNA(s)

J023108B15 - Rice Full-length cDNAs - Original: J023108B15

full reading [+] show sequence

GGTCTCTCTT CCCCTTCCAC GCCACCTCTC CCCTTCCCAC AGTCCCACGT 
CGTCGCCGGC GACACGCAGA CGAACGCGCC CGGCAAACCA TGGCGGCGCG 
ATCCGGACCA CCAGCCGCGG CCGTCGACCC CGAGGCCGTG CTCTCCCACA 
GCTTCCCCGA GGTCTCCTTC GCCTACGACG AGAGGGATGT GGCGCTGTAC 
GCGCTCGGAG TCGGGGCGTG CGGCGCGGAC GCCATCGACG AGAAGGAGCT 
GCACCTGGTG TACCACAGGG ACGGCCAGCC ACACATCAAG GCTCTTCCTA 
CTTTTGCTTC TTTATTTCCT TTCAAGAACA GCAATGGGCT TGGAATTGTT 
GATGTGCCTG GCCTTAACTT TGACGCAAGC CTTCTATTGC ATGGTCAACA 
TTACATAGAG ATATACAAGC CAATTCATTC GCGTGCCAAT GTTGTAAACA 
AGACCAAGAT AGCTGGCTTG CATGACAAAG GGAAAGCAAC TATTCTTGAG 
ATTGAGACTA CAACCCATGT CAAAGATTCT GGTGAAGTTT TATGTATGAA 
CAGGAGTACT ATCTACCTGC GTGGTGCTGG AGGCTTTTCT GATCCTTCAC 
GACCATACTC CTATGCTAGT TATCCCACTA ACCAGGTTTC TCGCATTTCT 
ATTCCAAATT CAGCGCCTTC TGCAGTATAT GAAGACCAGA CACAACAATC 
CCAGGCACTA TTGTACAGGT TATCTGGGGA CTATAATCCT TTGCATTCAG 
ACCCTATGGT TGCGCAGGTT GCAGGGTTCA CTCGTCCAAT ACTGCATGGT 
CTCAGTTCCC TTGGATTCGC GATCCGCGCT GTCATCAAAT CTTTCTGCAA 
TGGAGATCCA ACAGCAGTGA AAAGCATCTT TGGCCGTTTT CTTCTGCATG 
TCTATCCTGG AGAAACTTTA GTCACTGAAA TGTGGCTCCA AGGCCAGAGG 
GTGCTTTATC AAACAAAGGT CAAGGAACGC AACCGCGCCG TGCTTTCTGG 
ATATGTGTTG CTCAAACACA TCCCCTCATC ATTGTAAGTG CTGTATCATA 
TTCTTGACTG GATATTTTAT AAATTGGTGC CTGCCTGCTT GTATTAGTTT 
GGTAGTGTGT AGTGGTCAGA AAAGCTAAAG CTTTCCGGGG ACATAAAATC 
AGGTAAATGA AGAGCTTCAG CTACCGCAAG TGGTGGGTTG CCATTGTAAT 
GATAACAAGT GCTCTGCACC TATCTTCAAA TGATACATTG CCTAATTTCT 
CTTCTG

Gene models with high sequence identity

AT1G76150.1 [+] show detail - enoyl-CoA hydratase 2
E-value: 6e-109; Score: 391.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	no description	1.9e-36
Gene3D	no description	6.9e-37
superfamily	Thioesterase/thiol ester dehydrase-isomerase	2.6e-33
superfamily	Thioesterase/thiol ester dehydrase-isomerase	3.7e-25
HMMPfam	MaoC-like dehydratase	1.4e-22

See the detailed result >

AT1G76150.1

Model type: Protein coding
Short Description: enoyl-CoA hydratase 2
Curator Summary: Encodes a monofunctional enoyl-CoA hydratase 2, involved in the degradation of even cis-unsaturated fatty acids, gene expression is enhanced during the first 2 days of germination, as well as in senescent leaves.
Computational Description: enoyl-CoA hydratase 2 (ECH2); FUNCTIONS IN: oxidoreductase activity, 3R-hydroxyacyl-CoA dehydratase activity; INVOLVED IN: fatty acid beta-oxidation, unsaturated, even number, metabolic process; LOCATED IN: peroxisome; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: MaoC-like dehydratase (InterPro:IPR002539), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); Has 2242 Blast hits to 2242 proteins in 541 species: Archae - 23; Bacteria - 1245; Metazoa - 172; Fungi - 395; Plants - 47; Viruses - 0; Other Eukaryotes - 360 (source: NCBI BLink).
Link: InterPro Scan - TAIR

J033078P03 - Rice Full-length cDNAs - Original: J033078P03

full reading [+] show sequence

AAGTGCTGCT GCTGCAAAGG AAAAGAAGAA GCTGGAAAGG AAACTGGAGA 
AGGACCTCGA GAATGAGAAT GGAGGTGCTG GGGTCTATTC TGCTAGTCTC 
AAGAAGCATT ACTTATTGGC TGATGATGAA TGGAAGGAAG ATATTCTGCC 
TGAGATATTG GATGGGCATA ATGTTGCCGA TTTTCTGGAT CCAGATATAC 
TGCAGAGGTG TGAAGAGTTG GAGAGAGAGG AGGGTCTTCG CCTCGAAGAG 
GAAGCTGCAC AGGAAGCTTT CCAGATTGAT GGCCATGAAC TAACTGAAGA 
GCAGCGTGAA ATCTTGGGTC AGATTAGAAA GAAGAAGGCA TTGCTCATCC 
AAGAGCATAG AATGAAGAAG AGGACTGCAG AAAGCCGTCC TATTGTTCCT 
AGAAAGTTTG ACAAAGACAG GACATTCACA ACTAATAGAA TGGGTCGTCA 
ACTTTCATCC ATGGGTTTTG ATCCTAGGGC AGCTCTGGAC CGAGCCCGCA 
GCCGTTCTAG AGGTCGCAAG CGTGAGAGGT CACTTAGCAG AGCTGCTAGT 
GATGGTGATG ATATGGATAT AGATGGCCAG CAATCCAGCA AGAAATTGCG 
CGCGTTGTCG AGGTCCAGGT CTAGGTCAAA ATCAAGACCA CCTGAGGAGG 
TTGTTCCAGG AGAAGGATTC AAGGACTCTG CTCAGAAGAA GAAGGCGATT 
AAGAAAGCTA AGGATTCTGT GAGGAACAGG AACAAGGAGG CTCGTCGCGG 
TGAGGCGGAT CGTGTCATTC CAACTTTGAA ACCAAAGCAT CTATTCTCTG 
GAAAACGTTC TATTGGCAAG ACGAGCAGGC GATAAGTAAT TCAGCACTGC 
TGCTTAGCAT CACTGGTTTC AGGAAGAGCA GTATAATTGC AGTTCGGTGT 
TGGGATTAGG GTGAAGGAAG GCCTGTTTAT CCAATCTTTA GGACTTGTTC 
TACGCCGTTA TTCATACTGT CTGGTGGATT GGTGTTGTAT CGCGTCTCAT 
CCTTCGACTG AGACATAATT TTGTTAGCTT ATGTTTCAGC TTTATGCTAC 
ATTATATATG GTTTGGCTAG TATTAATAGA GTGGTTTAAT TTCGTCGAAT 
GGTACTCACT ACCATCAATA TCTTATAATT GTGACTTTGT TATACTTATA 
TTCCAGTCCT GTTCTACTGT ACCGATGCTC T

Gene models with high sequence identity

AT1G50920.1 [+] show detail - Nucleolar GTP-binding protein
E-value: 6e-55; Score: 211.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	NOG, C-terminal	1.5e-26
HMMPanther	PTHR11702	1e-38
HMMPanther	PTHR11702:SF4	1e-38

See the detailed result >

AT1G50920.1

Model type: Protein coding
Short Description: Nucleolar GTP-binding protein
Computational Description: Nucleolar GTP-binding protein; FUNCTIONS IN: GTP binding, nucleotide binding; LOCATED IN: membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GTP1/OBG (InterPro:IPR006073), Nucleolar GTP-binding 1 (InterPro:IPR010674), NOG, C-terminal (InterPro:IPR012973); BEST Arabidopsis thaliana protein match is: Nucleolar GTP-binding protein (TAIR:AT1G10300.1); Has 9379 Blast hits to 9179 proteins in 2035 species: Archae - 370; Bacteria - 5195; Metazoa - 1143; Fungi - 469; Plants - 325; Viruses - 0; Other Eukaryotes - 1877 (source: NCBI BLink).
Link: InterPro Scan - TAIR