K03819

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	stem (PO:0009047)	present in greater numbers in organism (PATO:0000470) (Stem::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K03819

Determination of introduced cDNA(s)

J023041B11 - Rice Full-length cDNAs - Original: J023041B11

full reading [+] show sequence

GGGACTCTCC CGATCCTTCC CCAACTCGGC GTCGCGCCGC CTCAACCGAA 
GCAAGGAGCA AAGCGGGGAT TCAGAACGGG AAGGAGAAGG TCCGGTTCGT 
CCGCTCTCGA GCTCAAGTGT CAGTTGAAGT GTTTTGAAAG TCATGGCGGA 
GCATTTGGCT TCAATATTTG GGACTGAGAA GGATAGGGTT AACTGCCCCT 
TTTACTTCAA GATTGGGGCT TGCCGTCATG GGGATCGTTG CTCCCGTCTG 
CACAACAGGC CAACTGTGTC GCCGACAATT GTGCTTGCTA ACATGTATCA 
GCGCCCTGAT ATGATCACAC CAGGAGTCGA TGCTCAAGGC CAACCTATTG 
ATCCTGAAAA GATGCAGGAG CACTTTGAGG ATTTCTATGA GGATATCTAT 
GAGGAGCTGA GCAAGTTTGG TGAGGTTGAG ACCCTCAATG TCTGTGATAA 
CCTTGCTGAC CACATGATAG GCAATGTGTA TGTCCAGTTC AGGGAGGAAG 
AACAGGCAGT CGCTGCGCAT AATGCCCTTC AAGGTCGCTT CTACTCAGGG 
CGCCCAATAA TTGTTGAGTA TTCCCCTGTG ACTGATTTCC GTGAGGCGAC 
CTGTAGGCAG TTTGAGGAGA ACAGCTGCAA CCGTGGGGGC TACTGTAATT 
TTATGCATGT AAAGCAGATT GGCAGGGAGC TCAGGAGGAA GCTTTATGGG 
GGGCGATCCA GGAGGAGCCA TGGGAGAAGC CGCAGCCCAA GCCCACGCCA 
CAGGAGGGGC AATCGTGACC GTGATGATTT CCGCCGTGAA CGTGATGGTT 
ATCGTGGTGG TGGTGATGGT TACCGTGGCG GCGGCGGTGG CGGTGGTGGT 
GACGGTTACC GCGGCGGTGA TAGTTACCGT GGTGGTGGTG GTGGTGGACG 
CAGGGGTGGG GGCAGCAGGT ATGACAGGTA TGATGATGGA GGAAGGCGCA 
GGCATGGCAG CCCCCCAAGG CGTGCAAGGA GCCCAGTCAG GGAAAGCAGC 
GAGGAACGCA GGGCGAAGAT TGAACAGTGG AACCGTGAAC GGGAGGAAAA 
GCCTTGAACG AAAGCAGTGA TATCTCTATG GATGGCCGGT TCATGCCTTC 
ATGGCAATCA CTATCCGCTG GAGTCGTGGT GTGCTGCTAT GTTCTAAGTG 
GATATTTAGT GTGTGTCGTT TGGTACTCAT GCTTGGCTGA GTAACCCTTT 
TAAATGCAGG ATTCTGGATT CTATTTACAT TGTGTTTCGT TTGACGTGTA 
AACCGCCGTT TGTTTTTAAA TATGCAATAT TGGATCTTTT GTCAGTCTAT 
TATCCTGTC

Gene models with high sequence identity

AT5G42820.2 [+] show detail - Zinc finger C-x8-C-x5-C-x3-H type family protein
E-value: 4e-97; Score: 352.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	Nucleotide-binding, alpha-beta plait	5.4e-38
ProfileScan	RNA recognition motif, RNP-1	11.145
ProfileScan	Zinc finger, CCCH-type	13.065
ProfileScan	Zinc finger, CCCH-type	11.892
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
FPrintScan	U2 auxiliary factor small subunit	3.4e-64
HMMPanther	U2 auxiliary factor small subunit	5.6e-120
HMMSmart	Zinc finger, CCCH-type	0.0068
HMMSmart	Zinc finger, CCCH-type	0.014
superfamily	RNA-binding domain, RBD	8.3e-25
superfamily	CCCH zinc finger	0.00056
superfamily	CCCH zinc finger	0.0031
HMMPfam	Zinc finger, CCCH-type	0.00000036
HMMPfam	Zinc finger, CCCH-type	0.00021
HMMPfam	RNA recognition motif, RNP-1	0.000013

See the detailed result >

AT5G42820.2

Model type: Protein coding
Short Description: Zinc finger C-x8-C-x5-C-x3-H type family protein
Curator Summary: U2 auxiliary factor small subunit. The atU2AF35b protein and its homolog, atU2AF35a, contain most of the conserved domains of hsU2AF35, including the psiRRM, one RS domain, two zinc fingers, and the two regions for interacting with U2AF large subunit. Both proteins lack the stretch of glycines present in human U2AF35. The sequences are overall 83% identical, and each Arabidopsis homolog shows approximately 70% similarity to hsU2AF35. U2AF(35) homologs were also identified from maize, rice and other plants with large-scale EST projects. Both genes are expressed in all major tissues, with atU2AF(35)a expressed at a higher level than atU2AF(35)b in most tissues. The expression patterns were different in roots: atU2AF(35)b expressed strongly in whole young roots and root tips and atU2AF(35)a limited to root vascular regions.
Computational Description: U2AF35B; FUNCTIONS IN: RNA binding, zinc ion binding, nucleotide binding, nucleic acid binding; INVOLVED IN: photoperiodism, flowering; LOCATED IN: nucleus; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, CCCH-type (InterPro:IPR000571), RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677), U2 auxiliary factor small subunit (InterPro:IPR009145); BEST Arabidopsis thaliana protein match is: U2 snRNP auxiliary factor small subunit, putative (TAIR:AT1G27650.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

J023099J20 - Rice Full-length cDNAs - Original: J023099J20

full reading [+] show sequence

GGCTCGATCA ATTCATCTCA TCGTCCATGG CGCCCAAGGT GGCGGTCACG 
GAGGCGACGG GGCGGCAGGC GGCGTCGTTC GTGCTCGGCT GCGTGGCCAC 
GCTCACCGTC ATGCTGCTCT TCCAGTACCA GGCGCCGCCG GACTACGGCC 
GCGCCGCCAG GTCGCCGGTG CAGTTCTCCA CCTCCAGAGA CCAGCTGCTG 
CTGCACTGCG GCGGCAATGG AACGGCGCCG CCGCCGCCGG TGATCGCACG 
TGGCGGCGAG GAGGCTAACA TCACCGGCAA GCCTCCGACG ACTGCTACTG 
CTGTCGCCGA GGAGCAGCCG CCAACCAAGC CTCCTGCGAC CTTTACTGCA 
TCTTCACCAA CTCATCATAT TCCAGCAACC AGTACAGATC TTGAAGAAGA 
GGGCGGCGAG TTCCGGGGGT TGGCGGCGGC GGTGGCGCGG GCGGCGACGG 
ATGACCGGAC GGTGATCATC ACGTGCGTGA ACCACGCGTT CGCGGCGCCC 
GACTCGCTCC TGGACATCTT CCTCCAGGGC TTCCGCGTCG GCGACGGCAC 
GCCGGAGCTC CTCCGCCACG TCCTCGTCGT CGCCATGGAT CCCACCGCGC 
TCACCCGGTG CCGCGCCGTC CACCCCCACT GCTACCTCTA CACCATGCCC 
GGCCTCGACG TCGACTTCAC CTCCGAGAAG TTCTTCGCCT CCAAGGACTA 
CCTCGAGCTC GTCTGGAGCA AGCTCAAGCT CCAGCGCCGA ATTCTCCAGC 
TCGGCTACAA CTTCCTCTTC ACGGACGTTG ACATTGTGTG GCTGAGGAAT 
CCGTTCAAGC ACGTGGCGGT GTACGCGGAC ATGGCGATCT CGAGCGACGT 
GTTCTTCGGT GACCCGGACA ACATCGACAA CTTCCCAAAC ACGGGCTTCT 
TCTACGTGAA GCCGAGCGCG AGGACGATCG CCATGACCAA GGAGTGGCAC 
GAGGCGAGGA GCTCGCACCC GGGGCTCAAC GAGCAGCCGG TGTTCAACCA 
CATCAAGAAG AAGCTGGTGA AGAAGCTGAA GCTCAAGGTT CAGTACCTGG 
ACACGGCCTA CATCGGCGGA TTCTGCAGCT ACGGCAAGGA TCTGAGCAAG 
ATCTGCACCA TGCACGCCAA CTGCTGCATC GGCCTCCAAT CCAAGATCAG 
TGATCTCAAG GGTGTTCTTG CTGACTGGAA GAACTACACC AGGTTGCCAC 
CCTGGGCAAA GCCCAATGCC AGGTGGACTG TGCCTGGTAA ATGCATCCAT 
TGATTTTGAT TGTCGATCGG GCAGTATCTG GTGTTGTAGG CTACGTACAG 
TCAGGAAGAT AGCTTTATTT GCACATGAAA GGGCGAATTT TGTTTTTTTT 
GGTTTGTTGT AGATTGATGG GTCGATTTCA AGTTATATTA CTGTATACCT 
GACGGCAGAC AAGTATACGC ATGATGGGCA AACTATATGA TATGATATGA 
TATGATAGTA CTTTAGATGT

Gene models with high sequence identity

AT4G19970.1 [+] show detail - CONTAINS InterPro DOMAIN/s: Nucleotide-diphospho-sugar transferase, predicted (InterPro:IPR005069...
E-value: 1e-67; Score: 254.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Nucleotid_trans	1.6e-64
HMMPanther	REGULATORY PROTEIN	2.1e-97
HMMPanther	PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN	2.1e-97
Coil	coiled-coil	0
Coil	coiled-coil	0

See the detailed result >

AT4G19970.1

Model type: Protein coding
Computational Description: CONTAINS InterPro DOMAIN/s: Nucleotide-diphospho-sugar transferase, predicted (InterPro:IPR005069); BEST Arabidopsis thaliana protein match is: Nucleotide-diphospho-sugar transferase family protein (TAIR:AT5G44820.1); Has 801 Blast hits to 466 proteins in 35 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 750; Viruses - 0; Other Eukaryotes - 49 (source: NCBI BLink).
Link: InterPro Scan - TAIR