K04032

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K04032

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K04032

Determination of introduced cDNA(s)

  • J033091I08 - Rice Full-length cDNAs - Original: J033091I08

    full reading [+] show sequence

    Nucleotide sequence: J033091I08 - full reading 
    GAAATCCCCC ATTCGAATCT CTCCGCCTCG TCGCCGTTGC GGCCGCTCCT 
    CCCCTCCCCT CTCTCTCTTG TTCCCCCATG GCGATCGCCA CCGAGTCGCG 
    CTGCGAGGAG GAGAAGGCTT CGCCGCATTC GCAGGAGGTG AAGCGGTGGG 
    TGTTGTCTGA TTTTGACATC GGGAAGCCCC TCGGGAGGGG CAAGTTCGGC 
    CATGTTTACT TAGCAAGAGA AAAGAGAAGT AACCATATTG TGGCTTTGAA 
    AGTACTTTTC AAGAGCCAAC TAAAGCAGTC CCAGGTGGAG CATCAGCTAC 
    GTCGGGAAGT TGAGATTCAG AGTCATCTTC GTCATCCCAA CATTCTACGG 
    CTGTATGGGT ATTTCTATGA TCAGACCCGT GTGTACTTGA TCTTGGAGTA 
    TGCCTTAAAA GGTGAACTTT ATAAGGAGTT GCAGAGGTGC AAGCATTTCT 
    CTGAAAGACG TTCAGCGACT TACATTGCAT CACTGGCGCA TGCGCTCATC 
    TACCTTCACG GAAAGCATGT GATCCATAGA GACATTAAAC CTGAAAATCT 
    TTTAATTGGG TCCCAGGGCG AACTGAAAAT TGCAGACTTT GGTTGGTCTG 
    TGCACACCTT CAACAGAAGA AGGACAATGT GTGGAACATT AGATTACCTG 
    CCCCCTGAAA TGGTGGAGAA GACTGAACAT GACTACCATG TTGACATTTG 
    GAGCTTGGGG ATCCTGTGCT ATGAGTTCCT TTATGGAGTC CCACCTTTCG 
    AAGCAAAAGA ACACTCCGAA ACATATCGCA GGATTGTGAA AGTTGACTTG 
    AAGTTCCCAC TGAAACCATT CGTGTCCCCG GCTGCGAAAG ACCTAATTTC 
    ACAGATGCTC GTGAAGAATT CGGCGCATCG TCTGCCCCTC CACAAGCTCC 
    TCGAGCACCC TTGGATAGTT CAAAACGCTG ATCCCTCCGG CGTGTACAGA 
    GGCTAGAACA ATCTCTTCCA CTCTCCTCAA TTCGAGGAGA GACCTGATTC 
    TTTTTCATCA CTGTTGTTAT GGCAAAACAA GAAAAAAGCA GTAGCCGTAC 
    TACTCTACCT GTTGTTTCCG ATCCCAGCTG CGCCTGTGTA GTTACGTGAC 
    AACGTGTATG CTCAAGCTTC AGATTTCCTC GATGAAGATT TGAAGCTGTG 
    TGTGTGGGTT TAACGCGTAT GTGTTTTAGC GTGTGTTCAC AGCATGGGGT 
    TGTGATATGT TTTCTGTGCA ACGCCCTGTA CTTAATGTAC TAATCGTATT 
    TCTTATTGGA CATCACTCGA TTAGTCT

    Gene models with high sequence identity

    1. AT4G32830.1 [+] show detail - ataurora1
      E-value: 6e-146; Score: 514.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Protein kinase, catalytic domain 49.163
    HMMPanther Spindle assembly checkpoint kinase 2.8e-137
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 2.8e-137
    Gene3D no description 4.6e-63
    HMMSmart Serine/threonine-protein kinase domain 3.1e-97
    HMMSmart Tyrosine-protein kinase, catalytic domain 1.9e-14
    HMMPfam Serine/threonine-protein kinase-like domain 8.6e-70
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    superfamily Protein kinase-like domain 9.9e-90

    See the detailed result >

    AT4G32830.1

    Model type
    Protein coding
    Short Description
    ataurora1
    Curator Summary
    Encodes a member of a family of Ser/Thr kinases whose activities peak during cell division. Transcripts are abundant in tissues rich in dividing cells like roots and flowers but are low or absent in fully expanded leaves and stems. In interphase cells, the protein is predominantly nuclear. During mitosis, the protein associates with plant-specific cytoskeletal structures (preprophase band, phragmoplast, nascent cell plate) that are necessary for cytokinesis as well as with the microtubule spindle. It specifically phosphorylates Ser10 of histone H3 and colocalizes with phosphorylated histone H3 during mitosis.
    Computational Description
    ataurora1 (AUR1); CONTAINS InterPro DOMAIN/s: Spindle assembly checkpoint kinase (InterPro:IPR020663), Protein kinase, ATP binding site (InterPro:IPR017441), Protein kinase, catalytic domain (InterPro:IPR000719), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: ataurora2 (TAIR:AT2G25880.1); Has 130781 Blast hits to 128539 proteins in 4385 species: Archae - 169; Bacteria - 15091; Metazoa - 48154; Fungi - 12951; Plants - 32302; Viruses - 558; Other Eukaryotes - 21556 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR