K04241

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	epinastic (PATO:0000945) (Cauline leaf::Shape::epinastic) abnormal (PATO:0000460) (Cauline leaf::Others)
	rosette leaf (PO:0000014)	increased width (PATO:0000600) (Rosette leaf::Shape::wide) upturned (PATO:0002031) (Rosette leaf::Shape::hyponastic)
	stem (PO:0009047)	present in fewer numbers in organism (PATO:0001997) (Stem::Number::few) abnormal (PATO:0000460) (Stem::Others)
T2
	whole plant (PO:0000003)	increased height (PATO:0000570) (Adult plant::Plant height::tall)
	rosette leaf (PO:0000014)	desaturated green (PATO:0001272) (Rosette leaf::Color::pale) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many) abnormal (PATO:0000460) (Rosette leaf::Others)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K04241

Determination of introduced cDNA(s)

J033121D04 - Rice Full-length cDNAs - Original: J033121D04

full reading [+] show sequence

GGTTTACACC TGCTCATCTT CGTCGCGACT CGGTGTTGGC TCCAGAAATC 
TCGCCAAGAG ACCATAAGCC CAATCCAAAT CTCTCCGCGT AGGAAGCAGA 
TGAAGATCAT CCCGGTCGCT TGCCTGGAGG ACAACTATGC CTACCTAATC 
GTGGACGAGA GCACCAAGTC GGCGGCGGCC GTCGACCCCG TGGAGCCGGA 
GAAGGTGCTT GCGGCGGCCG CGGAGGTCGG CGTCCGCATC GACTGCGTAC 
TCACCACCCA CCATCACTGG GATCATGCTG GTGGCAATGA AAAGATGGCG 
CAGTCAGTGC CAGGGATTAA GGTCTATGGA GGATCCCTGG ACAATGTGAA 
AGGATGCACT GATCAGGTGG AGAATGGAAC CAAATTGTCC CTTGGGAAGG 
ACATTGAGAT ACTATGCCTG CACACACCGT GCCACACAAA AGGACATATC 
AGCTATTACG TCACTAGTAA AGAGGAGGAA GATCCAGCAG TGTTTACTGG 
AGACACCTTG TTCATTGCTG GTTGTGGGAG ATTTTTTGAG GGCACTGCAG 
AGCAAATGTA CCAATCCCTT TGTGTAACAC TGGGCTCGCT GCCTAAGCCA 
ACTCAAGTTT ACTGCGGGCA TGAGTACACT GTGAAGAACC TGAAATTCAT 
ACTGACGGTC GAGCCAGATA ACGAAAAAGT GAAGCAGAAA CTAGAATGGG 
CTCAAAAGCA GCGTGAAGCA AACCAACCAA CTATTCCATC AACTATAGGA 
GAGGAATTTG AGACAAACAC CTTCATGCGT GTTGATCTGC CAGAAATACA 
GGCAAAGTTT GGTGCCAAGT CGCCAGTTGA GGCCCTGCGA GAAGTTAGGA 
AGACCAAGGA TAACTGGAAA AGTTAATACC GATGCAACTG TTACTCTTCG 
TCTGATGGTC CGGTCTCTCG AGGTTAGCTA ATGCAGAAAG CTTACCCTGT 
AGGTGGGGCT TACTGCCGTG CAACGCTTTA AGAGCGATGG TGTTAGGAAG 
AGTTTTAAGC TATAAATAAG CTCCATGTTA ATGTTCAAGG AGACATCATC 
AGATGTGCAT GTTAATGCTG GTCTTGTCTA TCACTGTACT ACATGAGCTA 
CGTATGAATT TTGGAGTTGG TCTTGTTTAA CTTTGAAAAG TCGAAAACGA 
GAGGTAGTAG TCATCCTCCA GTTTACATAT AAACGCATAA TAAGGGTGAA 
GTGAATCAAG ATTATTGTGC C

Gene models with high sequence identity

AT3G10850.1 [+] show detail - Metallo-hydrolase/oxidoreductase superfamily protein
E-value: 2e-113; Score: 406.00

InterPro Scan Digest

Program	Description	E-value
superfamily	Metallo-hydrolase/oxidoreductase	6.2e-71
HAMAP	Hydroxyacylglutathione hydrolase	36.257
HMMSmart	Beta-lactamase-like	1.1e-31
HMMPfam	Lactamase_B	1.9e-24
HMMPanther	HYDROXYACYLGLUTATHIONE HYDROLASE	9.5e-88
HMMPanther	BETA LACTAMASE DOMAIN	9.5e-88
Gene3D	no description	1.6e-82
HMMTigr	Hydroxyacylglutathione hydrolase	5.4e-95

See the detailed result >

AT3G10850.1

Model type: Protein coding
Short Description: Metallo-hydrolase/oxidoreductase superfamily protein
Curator Summary: glyoxalase II cytoplasmic isozyme (Glx2-2) mRNA, complete
Computational Description: GLY2; FUNCTIONS IN: hydroxyacylglutathione hydrolase activity; INVOLVED IN: methylglyoxal catabolic process to D-lactate; LOCATED IN: endomembrane system, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Beta-lactamase-like (InterPro:IPR001279), Hydroxyacylglutathione hydrolase (InterPro:IPR017782); BEST Arabidopsis thaliana protein match is: glyoxalase 2-4 (TAIR:AT1G06130.2); Has 13211 Blast hits to 13207 proteins in 2289 species: Archae - 256; Bacteria - 8297; Metazoa - 430; Fungi - 311; Plants - 206; Viruses - 0; Other Eukaryotes - 3711 (source: NCBI BLink).
Link: InterPro Scan - TAIR