K04247

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K04247

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K04247

Determination of introduced cDNA(s)

  • J013066F17 - Rice Full-length cDNAs - Original: J013066F17

    full reading [+] show sequence

    Nucleotide sequence: J013066F17 - full reading
    GGGGTTGGTG CTTGGCTAGC CACGGTATTA TTGCGTGAAA GAGGGGGAGG 
    TGAGGACGTG AGGTGAGGTG AGGTGAGGCA GAGTGACCAC AGCGACGGCA
    GCGTAGCCAT GGCGACCAGG CCGGGCCCTT TGACCGAATG GCCGTGGCAT
    AGGCTGGGGA ACTTCAAGTA CGTGGTGATG GCCCCCGTGG TGGCGCACGG
    CGCGCGCCGG GTGATGCGCA ACGGGTGGGG CGACCTCGAC ATCGCCTTCT
    CCCTCATCCT GCCGTCGCTG CTGCTGCGGA TGATCCACAA CCAGATCTGG
    ATCAGCCTCT CCCGCTACCA GACCGCCCGC AGCAAGCACC GCATCGTCGA
    CCGCGGCATC GAGTTCGGCC AGGTCGACCG CGAGCGAGGC TGGGATGACC
    AGATCCTGTT CAATGGGTTG GTGTTCTACG CGGGGTACCT GGCGATGCCG
    AGCGTGAGGC GGATGCCGGT GTGGAGGACG GACGGCGCCG TGGTGACGGC
    GCTGGTGCAC ACGGGCCCCG TCGAGTTCCT CTACTACTGG TTCCACCGCG
    CGCTGCACCA CCACTTCCTC TACTCCCGCT ACCACTCCCA CCACCACGCC
    TCCATCGTCA CCGAGCCCAT CACCTCTGTT ATTCATCCTT TTGCTGAACA
    TGTGGTCTAT TTCATACTGT TTGCAATCCC CATACTGTCA ACAATTTATT
    TGGGAAATGT TTCGGCCATG GGCATTGTGG GGTACATTGC TTACATTGAC
    TTCATGAACA ACATGGGACA CTGCAATTTT GAGTTGGTGC CAGAGTGGAT
    TTTCCAAATC TTTCCACCCC TTAAGTATCT GATCTACACC CCATCGTTCC
    ATTCTCTTCA TCATACGCAG TTCCGCACAA ACTACTCGCT ATTCATGCCA
    TTTTATGACT ACATATATAA CACCATGGAC AAGTCATCTG ACGAGCTGTA
    TGAAAGCTCA CTGAAAGGAA CGGAAGAAAC ACCTGACCTT GTTCATCTCA
    CGCATATGAC CAACTTGCAA TCAGCCTATC ATCTAAGGAT AGGAATTGCA
    TCCATAGCCT CCAAACCGTA CAGTGACTCT GCGTGGTACA TGTGGACGTT
    ATGGCCCTTG GCATGGCTGT CAATGGTACT TGCATGGATT TATGGGTCCT
    CAGCATTTGT CGTTGAGAGA ATTAAACTAA ACAAGATGAA GATGCAAACA
    TGGGCATTAC CAAGATACAA CTTCCAATAT GGCCTTACGT GGGAGAGAGA
    ACCAATCAAC GACTTAATTG AAAAGGCAAT ATTGGATGCT GATATGAAAG
    GGGTTAAAGT GATTAGTCTA GGACTACTGA ATCAGGCAAA GCAGCTCAAT
    GGGAACGGTG AACTATTTAG ACAGAAGTAT CCAAAATTAG GAGTTCGAAT
    TATCGACGGA AGTGGCTTAG CTACTGCAGT GGTGCTAAAA AGTATTCCTT
    CAGATGCAAA GAAAGTTTTT CTTCGTACAG GCACTTCTAA GATAGCTCGT
    GCCATTGCCA TTGCACTATG TGACAGAGGT GTCCAGGTGA TCATGAATGA
    AAAGGAGGTA TACCACATGC TCAAGTCACA AATACCAGAG AACAGGGCAA
    GCTATTTGAA ACTCTCGAGC GACAACGTAC CTCAGCTTTG GATAGTACAC
    AATATTGACG ACAACGAGCA AAAGATGGCG CCAAAAGGAA CAATTTTTAT
    TCCCATATCA CAGTTCCCCC TTAAGAAACT TCGCAAGGAC TGCACTTACA
    TGAGCACCCC AGCAATGAGG ATCCCGGAGG AAATGAAGAA TATCCACTCC
    TGCGAGAATT GGCTTCCAAG AAGGGTGATG AGTGCTTGGC ACATTGCTGG
    AATACTTCAT GCTCTGGAAG GATGGAACAT GCACGAGTGT GGGGATGAGA
    TGATGGATAT TGAGAAATCA TGGTCAGCTG CCATTCGGCA TGGATTCCTT
    CCTCTCACTA AAGCTTGACA CCTCTGGAAG CATGGATGGT GTATTCCATG
    CTAGTAGATG ATGCTATGAT TTCCAGCAAC GGCATAATCC ATTCATTATC
    TAGCTTGGGA ACCAGATGGA TTATTCACTT ATTATCTTAC TATGTTTGTA
    ATACTGAGCT TGGGTACTGA TAAGTCATGA GGAGGCACAG TGCAAACGAA
    CTTAATTCCA ATAGCTCAGT ATTTTATAAT CTATAGTCTA TGTCAGTTCT
    GTAACATTAC GTTTATCTCT TATTTGAATC TTGTGGGGGA ATAATTAAAA
    TAATAGGCAA TGTTTTCTTG TTTAGACTTC GCAAAGTAAA ACCTGACTGA
    TCGCACGGTC AGTGCTCTTG AGACGAGACA AACAAGACCA GGTAAAGCTA
    AGCTTACTCG CTGGCTCGCT GCTACTATCG TTTTAGCCTT CCCTTAAAGT
    TTGATTCGAC ACTGTAAAAT TCGCCCATGA CGCTCTGCAC AAAATCTTTG
    AAACTAGTGG CAACACAGCA AAATCAGATG C

    Gene models with high sequence identity

    1. AT1G02205.2 [+] show detail - Fatty acid hydroxylase superfamily
      E-value: 0; Score: 758.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther STEROL DESATURASE 2.4e-157
    HMMPanther STEROL DESATURASE 2.4e-157
    HMMPfam Uncharacterised protein, Wax2 C-terminal 2.3e-66
    HMMPfam Fatty acid hydroxylase 0.000000000000011

    See the detailed result >

    AT1G02205.2

    Model type
    Protein coding
    Short Description
    Fatty acid hydroxylase superfamily
    Curator Summary
    Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
    Computational Description
    ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR