K04247

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K04247

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K04247

Determination of introduced cDNA(s)

  • J013066F17 - Rice Full-length cDNAs - Original: J013066F17

    full reading [+] show sequence

    Nucleotide sequence: J013066F17 - full reading 
    GGGGTTGGTG CTTGGCTAGC CACGGTATTA TTGCGTGAAA GAGGGGGAGG 
    TGAGGACGTG AGGTGAGGTG AGGTGAGGCA GAGTGACCAC AGCGACGGCA 
    GCGTAGCCAT GGCGACCAGG CCGGGCCCTT TGACCGAATG GCCGTGGCAT 
    AGGCTGGGGA ACTTCAAGTA CGTGGTGATG GCCCCCGTGG TGGCGCACGG 
    CGCGCGCCGG GTGATGCGCA ACGGGTGGGG CGACCTCGAC ATCGCCTTCT 
    CCCTCATCCT GCCGTCGCTG CTGCTGCGGA TGATCCACAA CCAGATCTGG 
    ATCAGCCTCT CCCGCTACCA GACCGCCCGC AGCAAGCACC GCATCGTCGA 
    CCGCGGCATC GAGTTCGGCC AGGTCGACCG CGAGCGAGGC TGGGATGACC 
    AGATCCTGTT CAATGGGTTG GTGTTCTACG CGGGGTACCT GGCGATGCCG 
    AGCGTGAGGC GGATGCCGGT GTGGAGGACG GACGGCGCCG TGGTGACGGC 
    GCTGGTGCAC ACGGGCCCCG TCGAGTTCCT CTACTACTGG TTCCACCGCG 
    CGCTGCACCA CCACTTCCTC TACTCCCGCT ACCACTCCCA CCACCACGCC 
    TCCATCGTCA CCGAGCCCAT CACCTCTGTT ATTCATCCTT TTGCTGAACA 
    TGTGGTCTAT TTCATACTGT TTGCAATCCC CATACTGTCA ACAATTTATT 
    TGGGAAATGT TTCGGCCATG GGCATTGTGG GGTACATTGC TTACATTGAC 
    TTCATGAACA ACATGGGACA CTGCAATTTT GAGTTGGTGC CAGAGTGGAT 
    TTTCCAAATC TTTCCACCCC TTAAGTATCT GATCTACACC CCATCGTTCC 
    ATTCTCTTCA TCATACGCAG TTCCGCACAA ACTACTCGCT ATTCATGCCA 
    TTTTATGACT ACATATATAA CACCATGGAC AAGTCATCTG ACGAGCTGTA 
    TGAAAGCTCA CTGAAAGGAA CGGAAGAAAC ACCTGACCTT GTTCATCTCA 
    CGCATATGAC CAACTTGCAA TCAGCCTATC ATCTAAGGAT AGGAATTGCA 
    TCCATAGCCT CCAAACCGTA CAGTGACTCT GCGTGGTACA TGTGGACGTT 
    ATGGCCCTTG GCATGGCTGT CAATGGTACT TGCATGGATT TATGGGTCCT 
    CAGCATTTGT CGTTGAGAGA ATTAAACTAA ACAAGATGAA GATGCAAACA 
    TGGGCATTAC CAAGATACAA CTTCCAATAT GGCCTTACGT GGGAGAGAGA 
    ACCAATCAAC GACTTAATTG AAAAGGCAAT ATTGGATGCT GATATGAAAG 
    GGGTTAAAGT GATTAGTCTA GGACTACTGA ATCAGGCAAA GCAGCTCAAT 
    GGGAACGGTG AACTATTTAG ACAGAAGTAT CCAAAATTAG GAGTTCGAAT 
    TATCGACGGA AGTGGCTTAG CTACTGCAGT GGTGCTAAAA AGTATTCCTT 
    CAGATGCAAA GAAAGTTTTT CTTCGTACAG GCACTTCTAA GATAGCTCGT 
    GCCATTGCCA TTGCACTATG TGACAGAGGT GTCCAGGTGA TCATGAATGA 
    AAAGGAGGTA TACCACATGC TCAAGTCACA AATACCAGAG AACAGGGCAA 
    GCTATTTGAA ACTCTCGAGC GACAACGTAC CTCAGCTTTG GATAGTACAC 
    AATATTGACG ACAACGAGCA AAAGATGGCG CCAAAAGGAA CAATTTTTAT 
    TCCCATATCA CAGTTCCCCC TTAAGAAACT TCGCAAGGAC TGCACTTACA 
    TGAGCACCCC AGCAATGAGG ATCCCGGAGG AAATGAAGAA TATCCACTCC 
    TGCGAGAATT GGCTTCCAAG AAGGGTGATG AGTGCTTGGC ACATTGCTGG 
    AATACTTCAT GCTCTGGAAG GATGGAACAT GCACGAGTGT GGGGATGAGA 
    TGATGGATAT TGAGAAATCA TGGTCAGCTG CCATTCGGCA TGGATTCCTT 
    CCTCTCACTA AAGCTTGACA CCTCTGGAAG CATGGATGGT GTATTCCATG 
    CTAGTAGATG ATGCTATGAT TTCCAGCAAC GGCATAATCC ATTCATTATC 
    TAGCTTGGGA ACCAGATGGA TTATTCACTT ATTATCTTAC TATGTTTGTA 
    ATACTGAGCT TGGGTACTGA TAAGTCATGA GGAGGCACAG TGCAAACGAA 
    CTTAATTCCA ATAGCTCAGT ATTTTATAAT CTATAGTCTA TGTCAGTTCT 
    GTAACATTAC GTTTATCTCT TATTTGAATC TTGTGGGGGA ATAATTAAAA 
    TAATAGGCAA TGTTTTCTTG TTTAGACTTC GCAAAGTAAA ACCTGACTGA 
    TCGCACGGTC AGTGCTCTTG AGACGAGACA AACAAGACCA GGTAAAGCTA 
    AGCTTACTCG CTGGCTCGCT GCTACTATCG TTTTAGCCTT CCCTTAAAGT 
    TTGATTCGAC ACTGTAAAAT TCGCCCATGA CGCTCTGCAC AAAATCTTTG 
    AAACTAGTGG CAACACAGCA AAATCAGATG C

    Gene models with high sequence identity

    1. AT1G02205.2 [+] show detail - Fatty acid hydroxylase superfamily
      E-value: 0; Score: 758.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther STEROL DESATURASE 2.4e-157
    HMMPanther STEROL DESATURASE 2.4e-157
    HMMPfam Uncharacterised protein, Wax2 C-terminal 2.3e-66
    HMMPfam Fatty acid hydroxylase 1.1e-14

    See the detailed result >

    AT1G02205.2

    Model type
    Protein coding
    Short Description
    Fatty acid hydroxylase superfamily
    Curator Summary
    Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
    Computational Description
    ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR