K06646

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K06646

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K06646

Determination of introduced cDNA(s)

  • J023030K20 - Rice Full-length cDNAs - Original: J023030K20

    full reading [+] show sequence

    Nucleotide sequence: J023030K20 - full reading 
    TTGAAGTAAT CGGCGCTTTC GCGAGAGAGA GCGATACGAA CAAGAGAAAA 
    TCTTCTCCAG ACACCGCGGC GGGCTTCCCC ACCCCATCGG AAGGAAGAAG 
    ACGACCAGCT CGCGCGCTCG GATCTCAGCC ATGGAGAAGG CGATCGATCG 
    CCAGCGCGTC CTCCTCGCCC ACCTCCTCCC CTCCTCCTCC TCCGACCAAT 
    CGCTCCTCTC CGCGTCGGCG TGCGCCGCCG GGGACAGCGC CGCCTACCAG 
    AGGACTTCCG CCTACGGGGA CGACGTCGTC GTCGTCGCTG CCTACAGGAC 
    ACCCATATGC AAGGCCAAGC GAGGAGGTTT CAAGGATACA TACCCAGAGG 
    ACCTTCTTAC TGTTGTTCTA AAGGCTGTTC TGGACAACAC TAAGATCAAC 
    CCTGGTGAAA TTGGTGACAT TGTAGTTGGC ACGGTGCTAG GTCCAGGCTC 
    GCAGCGTGCA ATCGAGTGCA GGGCTGCGGC TTTCTATGCT GGAGTTCCCG 
    AAAACGTTCC TGTTAGAACT GTCAACCGGC AATGTTCCTC TGGATTACAG 
    GCAGTGGCTG ATGTTGCCGC GGCCATTAAA GCTGGGTTCT ACGACATAGG 
    GATTGGTGCA GGCCTGGAAT CCATGTCAGT AAATGCTATG GGTTGGGAAG 
    GACAAGTAAA CCCTAAAGTA AATGAAGTCC AGAAAGCACA GGATTGTCTT 
    TTGCCCATGG GGATAACGTC CGAAAATGTT GCGCACCGAT ATGGTGTAAC 
    AAGACAAGAG CAGGATCAGG CTGCTGCTGA GTCTCATAGG AGGGCTGCTG 
    CAGCCACAGC TGCTGGGAAA TTCAAAGATG AGATTGTTCC GGTGCCTACA 
    AAGATTGTTG ACCCCAAGAC TGGAGAGGAA AAGAAAGTTG TGATATCAGT 
    TGATGATGGA ATTAGGCCAG GGACCACAGC ATCTGGATTG GCAAAGCTTA 
    AACCAGTTTT CCGGAAGGAT GGTACCACAA CTGCAGGCAA CTCTAGTCAA 
    GTGAGCGATG GTGCTGGAGC TGTTCTTCTC ATGAGGAGGG ACGTAGCGAT 
    GAAGAAGGGA CTTCCTATTC TTGGTGTTTT CAGGAGCTTT GCTGCTGTTG 
    GAGTAGATCC TGCTGTTATG GGTGTTGGTC CTGCAGTAGC AATACCAGCT 
    GCAGTGAAGT CTGCTGGACT CCAGATTGAA GACATTGATC TGTTTGAATT 
    GAATGAGGCC TTCGCTTCGC AGTTCGTGTA TTGCTGCAAC AAGCTGGGAC 
    TGGACAGGTC AAAAGTAAAC GTGAATGGAG GCGCAATCGC CCTAGGACAT 
    CCTCTTGGTG CAACAGGTGC CCGGTGCGTC GCAACTCTTC TGAATGAAAT 
    GAAGCGCCGG GGCAGGGACT GCAGATTCGG TGTTGTCACC ATGTGCATTG 
    GATCTGGCAT GGGGGCAGCA GCTGTTTGAG CGGGGTGACT CGGTCGACCA 
    ATTCTCAAAC GTGCGCCACC ATTAGCGGCA GTTTTCTAAG AGAAATGCAA 
    AGCAAAGCAG TGGTGCAAAA TCATCATGGT GGTCAGTCCA ATGGAGCATA 
    CTCTGGCAGT AAGATCTATA CCGAAAACTA AATGCAGGAA ACACGTAATC 
    TTCTCCGGTG AAAGGTGTAA CAGTATATTG AAGATCTTTT CATGTAACAT 
    TTTGCACTAA TAATAATTTA TGCGAATAAG GGGCATGCAT GCTCAATGTT 
    TGTTTTGTCA AGTGTTCCAC ACGAAACACT GAGAGGAGCA CAGTGGTTGC 
    AAATGTTGAA GGGCCTGATA AGGGTAGGCC TGATCATGGA TAATTTGGCC 
    CAAATCAAAT CTGGCCTGGC CTGATCGTTG TCCAAAATAC TTAGTACTAC 
    TTTCTCTGTA AAAAAATAAT ACAGTATAAA ACAATTTATT TTGCTTGTTA 
    GGACAATGCT TTAATCACCA ACTGCTTTAT TAATATATTA TATTTATGTG 
    ACACAGAAGT ATAATTACAG GAAAGCATTT GTGAATATAA ATCTAATAGC 
    ACTCATTACT TTTGAATGTA AATCTAATGA CATTAATTTT GTCTC

    Gene models with high sequence identity

    1. AT2G33150.1 [+] show detail - peroxisomal 3-ketoacyl-CoA thiolase 3
      E-value: 7e-150; Score: 24.60

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Thiolase, acyl-enzyme intermediate active site 0
    PatternScan Thiolase, active site 0
    PatternScan Thiolase, conserved site 0
    HMMTigr Thiolase 3e-189
    Gene3D Thiolase-like, subgroup 8.8e-63
    HMMPanther 3-KETOACYL-COA THIOLASE B (BETA- KETOTHIOLASE 4.4e-214
    HMMPanther Thiolase 4.4e-214
    HMMPfam Thiolase, N-terminal 1.5e-81
    HMMPfam Thiolase, C-terminal 1.5e-47
    superfamily Thiolase-like 3.2e-72
    superfamily Thiolase-like 6.1e-38

    See the detailed result >

    AT2G33150.1

    Model type
    Protein coding
    Short Description
    peroxisomal 3-ketoacyl-CoA thiolase 3
    Curator Summary
    Encodes an organellar (peroxisome, glyoxysome) 3-ketoacyl-CoA thiolase, involved in fatty acid b-oxidation during germination and subsequent seedling growth. Mutants have defects in glyoxysomal fatty acid beta-oxidation. EC2.3.1.16 thiolase.
    Computational Description
    peroxisomal 3-ketoacyl-CoA thiolase 3 (PKT3); FUNCTIONS IN: acetyl-CoA C-acyltransferase activity; INVOLVED IN: fatty acid beta-oxidation, jasmonic acid biosynthetic process, response to wounding, fatty acid oxidation, glyoxysome organization; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase (InterPro:IPR002155), Thiolase, active site (InterPro:IPR020610), Thiolase, N-terminal (InterPro:IPR020616), Thiolase, conserved site (InterPro:IPR020613), Thiolase, C-terminal (InterPro:IPR020617), Thiolase-like, subgroup (InterPro:IPR016038), Thiolase-like (InterPro:IPR016039), Thiolase, acyl-enzyme intermediate active site (InterPro:IPR020615); BEST Arabidopsis thaliana protein match is: peroxisomal 3-ketoacyl-CoA thiolase 4 (TAIR:AT1G04710.1); Has 22382 Blast hits to 22371 proteins in 2261 species: Archae - 414; Bacteria - 14116; Metazoa - 985; Fungi - 655; Plants - 282; Viruses - 0; Other Eukaryotes - 5930 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR