K08123

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K08123

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K08123

Determination of introduced cDNA(s)

  • J023084C02 - Rice Full-length cDNAs - Original: J023084C02

    full reading [+] show sequence

    Nucleotide sequence: J023084C02 - full reading
    GCTCCGGTTT TCTCCGCAGT TTCTTGATCC ATCCGCCTTT CCTCTCGCAG 
    CCGTCGGCGG GGGGAGTGGT TAGTTGGTTA CTGGCTTGCA GAGTTTTCAG
    AGAGATTTAG GCAATGGCGG AATCAGGCCA ATCGATTGCG AGTGATGTGA
    CTGCACTGAT CGGCAATACT CCCCTGGTGT ACCTGAACAA GGTGGTGGAT
    GGATGTGAGG CCCAAATCGC TGCCAAGCTT GAGATCATGG AGCCCTGCTC
    CAGTGTCAAG GATAGGATTG GTTATAGCAT GATTACAGAT GCAGAAGAGA
    AGGGTCTCAT TACTCCAGGA AAGAGTGTTT TGATCGAGCC TACTAGTGGA
    AATACTGGTA TTGGCCTTGC TTTCATGGCT GCTGCCAAGG GTTATAAGCT
    AATCCTGACA ATGCCAGCAT CCATGAGCAT GGAAAGGAGG ATCATTCTTA
    AGGCCTTCGG TGCCGAGTTG GTCCTTACCG ATCCACTTCT GGGCATGAAA
    GGTGCAATCC AGAAGGCAGA TGAGCTGGCA GCAAAGATGC CTAACTCTTA
    CATTCTCCAG CAGTTTGAGA ACCCTGCAAA CCCAAAGATC CACTATGAGA
    CAACTGGACC TGAGATTTGG AAGGCCACTG CTGGGAAAGT TGATATCCTT
    GTCTCTGGCA TTGGAACCGG TGGTACAGTA ACCGGAGCAG GAAAGTACCT
    CAAGGAGCAG AACCCTGAGA TCAAGATCTA TGGTGTTGAA CCGACAGAAA
    GTGCAATTTT ATCTGGAGGA AGACCTGGTC CACACAAAAT CCAAGGTATA
    GGTGCTGGTT TTGTTCCTGG TGTACTTGAT GTCAATCTCC TGGATGAAGT
    GGTCCAAGTC TCAAGTGATG AAGCCATCAG CATGGCGAAG CAGCTAGCGT
    TGAAAGAAGG GCTGCTGGTT GGGATCTCAT CTGGTGCTGC TGCAGTCGCT
    GCCATCAGGG TCGCGCAGCG GCCCGAGAAC AAAGGGAAGC TCGTAGTTGT
    GGTCTTCCCG AGCTTCGGTG AGCGCTACCT GTCGTCTGTC CTCTTCGAAT
    CGATCAAGAG GGAGGCCGAA AACATGGTGT TCGAGCCATG AACGGCTCCA
    GCATTGTGCA AATTGTGCTT CCATGGTTTA GGGGGCTTGG AGAGAATGGC
    GTCCCGAGTG CATTTCTTCA TGTTGGTCGC ACTTGCCATC ATGTAGTCGC
    TTGATCTGAA TAAAGAACTT ACATACATCA ATCAAAGTCT GGGGATTCGA
    TCAGCTTTCC TGGTGTTATA TGAGCAGTTC ATATGTGTAC TTTCTTCCAT
    ACTGAAATGT GTATCTTAGC TACTGAAGCT TCCATGG

    Gene models with high sequence identity

    1. AT4G14880.4 [+] show detail - O-acetylserine (thiol) lyase (OAS-TL) isoform A1
      E-value: 4e-129; Score: 458.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 1.4e-68
    HMMTigr Cysteine synthase K/M 9.2e-209
    HMMTigr Cysteine synthase A 2.4e-216
    superfamily Pyridoxal phosphate-dependent enzyme, beta subunit 4.6e-92
    PatternScan Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site 0
    HMMPanther CYSTEINE SYNTHASE 1.8e-151
    HMMPanther SER/THR DEHYDRATASE, TRP SYNTHASE 1.8e-151
    HMMPfam Pyridoxal phosphate-dependent enzyme, beta subunit 1.4e-57

    See the detailed result >

    AT4G14880.4

    Model type
    Protein coding
    Short Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1
    Curator Summary
    Encodes a cytosolic isoform of cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis and for the fixation of inorganic sulfide. It catalyzes the formation of cysteine from O-acetylserine and inorganic sulfide. Gene expression is predominant in the root cortex and the xylem parenchyma. Gene expression is induced in leave, stems and roots by high salt and heavy metal stresses, mediated by ABA. Lines carrying semi-dominant mutations exhibit early senescence.
    Computational Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1 (OASA1); CONTAINS InterPro DOMAIN/s: Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site (InterPro:IPR001216), Cysteine synthase A (InterPro:IPR005859), Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Cysteine synthase K/M (InterPro:IPR005856); BEST Arabidopsis thaliana protein match is: O-acetylserine (thiol) lyase (OAS-TL) isoform A2 (TAIR:AT3G22460.1).
    Link
    InterPro Scan - TAIR