K08326

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K08326

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K08326

Determination of introduced cDNA(s)

  • J023010C06 - Rice Full-length cDNAs - Original: J023010C06

    full reading [+] show sequence

    Nucleotide sequence: J023010C06 - full reading 
    GGGCGGCCTC CTCCTCTCCT CCTCCCCTGC ACAGACCACT CGTTTCCTCC 
    ACAAAGAGGG AGGGAACAAG GGAAGGGTGT CGCCCGCCCC CCACCCCGAT 
    CTGCCTCCGC CGCTCCGCTC CTCCGCGCCT GCGAAATCTA CCAACGCTAA 
    CTCAGCAAGA TGGGTGCCGG CGGCAGGATG ACGGAGAAGG AGCGGGAGGA 
    GCAGCAGAAG CTGCTCGGCC GCGCCGGCAA TGGCGCGGCC GTGCAGCGGT 
    CGCCGACGGA CAAGCCGCCG TTCACGCTGG GGCAGATCAA GAAGGCCATC 
    CCGCCTCACT GCTTCCAGCG CTCGGTGATC AAGTCCTTCT CCTACGTGGT 
    CCATGACCTC GTGATCGTCG CCGCGCTGCT CTACTTCGCG CTGGTCATGA 
    TCCCCGTGCT GCCGAGCGGG ATGGAGTTCG CGGCATGGCC GCTCTACTGG 
    ATCGCGCAGG GCTGCGTGCT CACCGGCGTG TGGGTCATCG CGCACGAGTG 
    CGGCCACCAT GCCTTCTCCG ACTACTCGGT GCTCGACGAC ATCGTCGGCC 
    TCGTGCTGCA CTCGTCGCTG CTCGTCCCCT ACTTCTCGTG GAAGTACAGC 
    CACCGGCGCC ACCACTCCAA CACCGGGTCG CTGGAGCGCG ACGAGGTGTT 
    CGTCCCGAAG CAGAAGTCGG CGATGGCGTG GTACACCCCG TACGTGTACC 
    ACAACCCGAT CGGCCGGCTG GTGCACATCT TCGTGCAGCT CACCCTCGGG 
    TGGCCGCTGT AACTGGCGTT CAACGTGTCC GGCCGCCCGT ACCCGCGCTT 
    CGCGTGCCAC TTCGACCCCT ACGGCCCGAT CTACAACGAC CGGGAGCGCG 
    TCCAGATCTT CATCTCCGAC GTCGGCGTCG TGTCCGCGGG GCTCGCCCTG 
    TTCAAGCTGT CGTCGGCGTT CGGGTTCTGG TGGGTGGTGC GCGTCTACGG 
    CGTGCCGCTG CTGATCGTGA ACGCGTGGCT GGTGCTCATC ACCTACCTGC 
    AGCACACCCA CCCGGCGCTG CCGCACTACG ACTCGAGCGA GTGGGACTGG 
    CTCCGCGGCG CGCTGGCCAC CGTGGACCGC GACTACGGCA TCCTCAACAA 
    GGTGTTCCAC AACATCACGG ACACGCACGT CGCGCACCAC CTCTTCTCCA 
    CCATGCCGCA CTACCACGCC ATGGAGGCCA CTAAGGCGAT CCGCCCCATC 
    CTCGGCGAGT ACTACCAGTT CGACCCGACG CCCGTCGCCA AGGCGACATG 
    GCGCGAGGCC AAGGAGTGCA TCTACGTCGA GCCTGAGGAC AACAAGGGCG 
    TCTTCTGGTA CAACAACAAG TTCTAACTGC TGCTGCTGTG AAATCAGCAT 
    CACACATCCA TAGCCAAGCA GCAAACAAAT TTGAAGAAGA AGATTACAAG 
    GGAAGAGAAG ATAGTGTCTT CGGAAATCGT CGTAGCAAGT ATCCATCCAT 
    CCATCCAACC CATGAACAAT CGTCTATCTA TCCATGCATC TATCTATGGT 
    TAGTCTCTTT AGATAGGAGA GGGCACTTGG GCACAGAGGA AGGCTATTGC 
    AGTGCCATTG CTAGAGTTGC CATCAAGTGC AAAGTAGGCG GATCAGGCGT 
    GTGTCATGCC TGTGGATTTG TAGTCTATGT ATGTGTCAGC TGCTGAGCTC 
    CGGTGTCGCA GCCTTGGTCC CTGTCGTGTT ATTTCCATCG TTTTTTTCCC

    Gene models with high sequence identity

    1. AT3G12120.2 [+] show detail - fatty acid desaturase 2
      E-value: 1e-160; Score: 563.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Fatty acid desaturase, type 1 3e-15
    HMMPfam Protein of unknown function DUF3474 3.1e-12
    HMMPfam Fatty acid desaturase, type 1 3.5e-15

    See the detailed result >

    AT3G12120.2

    Model type
    Protein coding
    Short Description
    fatty acid desaturase 2
    Curator Summary
    Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
    Computational Description
    fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR