K10021

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K10021

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K10021

Determination of introduced cDNA(s)

  • J033094J24 - Rice Full-length cDNAs - Original: J033094J24

    full reading [+] show sequence

    Nucleotide sequence: J033094J24 - full reading 
    GGGGCTTGTC CTTTGGAAAA TTTCTGGAAC TTAGCTTCTC AAATCATGCG 
    GCAGCTAGTA GGGTAGCCAG TTGTGGCCAT TCTCTTCACA GGGATTGCCT 
    TCGTTTTTAC GGCTTTGGTG AAATGGTTGC TTGCTTCCGA TATGCATCGA 
    TCAAGGTTCA TTCAGTGTAT TTGCCACCAC CGAAACTTGA TTTCACTTCT 
    CAGCACCAGG AATGGGTAGA GCAAGAAGCA AATGAGGTGG TTGACTCAGC 
    TGAACTTCTG TTCACTGAAG TACTGAATGC ACTCCACCAA ATTTCAGAGG 
    GAAGGCCAAT TACAGGTTCT TTTGATGGCA ACATGAAAAT CCTCGAGTTA 
    AGAAGGAACA TCATGGAGCT GGAAGAGATC CTGCAAATAG AGAAAGCAGA 
    TTTTACGGAA TCATTGAAGA ATCTACTGAA CAAGGAGATC AGAAAGGGGA 
    AGCCATTCAT TGATATTCTT GAGGTTAACA AACTAAGGAG GCAGCTACTG 
    TTTCTGTGTT ACCTGTGGGA TCAGCGGCTA ATCTTTATAG CAAATTCAGG 
    TGGCAAGTAC TGTGACACAC TAGGAGGTTT ACGGGTTGGG AGTAGGAGTT 
    CTGACTCTAA CGATAAATCA GTTGATACCA ATGCAACTAC AAAACTAGAG 
    AAGAGCTCAA AGGGCTCCGA AGTTCTTTCA AATGCTAAGG AAGGATCCTT 
    GAAGCAGAGC CAAAGTCCAC TTCATGCTAA TGACAAGGAG CCCAACCAAC 
    CTGATCAATC TAATGGAAAT AGTTCAAGGA TTGGTGCAGG GTTGAATGGC 
    ATGGAAGATG CGATTGCCAA AATCAATCAT TCTAACAGCG CAGATGTTAA 
    GGATAACCTG GATCATCAAG AATCCAGTAT TGGTGTTCGT AGGGTTCTTT 
    CTGATGGTCA GTTTCCAGTA AATGCTGATA TTTCAGACAC ATTGGATGCA 
    AAATGGAGAG GTGAAAATGG AACAGTTCCT GATACAAGTA TCCTGAAACC 
    ATTAGCTCTG CTAGAAGGTT CAGCTGATCT TAAAAACCAA GCTAAGGCAG 
    TGCCAACCAA TGCTTCTTTA TCTGTCAGGT CTGGAGATAC AGTAGAAGAC 
    TTATCAAGCT GGCTCAAAAT GCCATACATG ACATTCTATG ACTCCTTAAA 
    CACAAATTCT GGGACAGCAC CGAGATTTGG GGCCCTGGCT GACTACAATC 
    CTGTGTACAT TACATTGTTC CGTGAGTTAT CACAGCAAGG TGGAGCAAGA 
    CTTTTTCTGC CAACTGGAGC TAATGATGTT GTTATTCCAG TATTTGATGA 
    TGAACCGACC AGTATAATTT CTTATGCACT TGTTTCACCC ATGTACTGTC 
    ACCAAATGTT GGATGAGAAC AGCAAGAACA AAGATGGTCC AGATTCTTCA 
    CTTCCATTGC CTGTATATGA TTCTGGAAAT TTTAATCCTT TTCATTTATT 
    TGAGGATTTC GGATCCGCTG ATGATCTTGC TTCGTCAATA TCTGGAACCA 
    GAGGGTCATT TGTTCCCGAT CTAGTTCACC TAAGAGTTCC CTTTGAGGAT 
    GGTGGCCCAC TCGGGAAGGT GAAATACACA GTGACGTGCT ACTATGCGAA 
    AAGCTTTGAA GCTCTTAGAA GGTCTTGCTG TCCATCTGAA CTTGACTTTT 
    TAAGATCTAT CAGTCGATGC AAGAAATGGG GAGCACAAGG TGGCAAAAGC 
    AATGTGTTTT TTGCAAAATC TCTGGATGAT AGATTTATAA TAAAGCAGGT 
    CACAAAAACA GAACTTGAAT CATTCCTTAA GTTTGGACTA GATTATTTTA 
    AATATTTATC AGAGTCTATA AGCACCGGAA GCCCTACATC CCTTGCAAAG 
    ATTCTGGGAA TCTATCAGGT TACAATTAAG CATGTGAAGG GTGGCAAGGA 
    GTCAAAAATG GATTTACTTG TGATGGAAAA TCTTTTATTT GGACGGAATA 
    TTACAAGGCT CTATGATCTC AAGGGATCTT CGAGGTCAAG GTATAATGCT 
    GATTCCAGCA GCAACAAGGT TCTTCTTGAC CAGAACTTGA TTGAGGCAAT 
    GCCAACGTCA CCAATTTTCG TTGGGAACAA GGGAAAGAGG CTTCTGGAAA 
    GAGCTGTTTG GAATGATACT TCCTTTCTGG CAGGAATTGA TGTGATGGAT 
    TACTCATTGC TTGTGGGAGT TGACGAGGAG AAACATGAAT TGGTTCTGGG 
    AATAATTGAT TTTATGAGGC AGTATACATG GGACAAGCAC CTCGAGACAT 
    GGGTCAAATC GTCCGGTATT CTCGGCGGGC CGAAGAATGC CCCTCCAACT 
    GTGATTTCAC CCATGCAGTA TAAGAAGCGG TTTAGAAAAG CTATGTCTGC 
    TTATTTTATT GTAATTCCGG AGCAATGGAT GCCTGCAATA ATCAATCCGA 
    GCAAATCATC ATCAAATCTT TGCCAAGAAG ATCCTCAAAA TGCTTCACAA 
    GAGTAGTACA ATATTCAGTT CATTATGCCA GGCAGAGAGC ACCTGGTTTT 
    TTGTCTTGAT CCAATCAAAA GCGCCAAACG ATCAGTCTGG ATGCTGCGGC 
    ATCAATGTGG TATTACGACT GCAATTATCA AAATGCTTGG ACTCTCATTT 
    GCACAGATCC GATTGCAGTG GAGTCGTCTT GGCTCAAGAA TCATCGGGTG 
    TATATATCCT TGAAACTGTA ATTATGCATT AGTTTTTTTA TTTTCTTTTT 
    GATTTATTAT GTAATAATAT AATGTCGTCG CTTCTCTCTA GTTCCCATTC 
    CATTTTGGTT GGATGTAAAC AATAGAAGTG GGGCATTTTT TTTTTGTAAG 
    TGCTCGTTGT ATATTATTAT ATATATATGC CATGTTATTT

    Gene models with high sequence identity

    1. AT3G14270.1 [+] show detail - phosphatidylinositol-4-phosphate 5-kinase family protein
      E-value: 0; Score: 775.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup 3.4e-118
    Gene3D no description 8.7e-20
    Gene3D no description 2.2e-36
    ProfileScan Phosphatidylinositol-4-phosphate 5-kinase, core 55.573
    HMMPfam Phosphatidylinositol-4-phosphate 5-kinase, core 3.3e-64
    HMMPanther Phosphatidylinositol-4-phosphate 5-kinase, core 1.4e-25
    Coil coiled-coil 0
    superfamily SAICAR synthase-like 5.8e-75

    See the detailed result >

    AT3G14270.1

    Model type
    Protein coding
    Short Description
    phosphatidylinositol-4-phosphate 5-kinase family protein
    Curator Summary
    Encodes a protein that is predicted to act as a 1-phosphatidylinositol-3-phosphate (PtdIns3P) 5-kinase based on its homology to Fab1 from yeast. It contains an FYVE domain required for binding to PtdIns3P-containing membranes in yeast, as well as a Cpn60_TCP1 homology domain plus a kinase domain. fab1a/fab1b pollen grains not viable and have defective vacuolar organization.
    Computational Description
    FORMS APLOID AND BINUCLEATE CELLS 1B (FAB1B); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases (TAIR:AT4G33240.2); Has 8765 Blast hits to 8134 proteins in 458 species: Archae - 587; Bacteria - 13; Metazoa - 3836; Fungi - 1744; Plants - 978; Viruses - 3; Other Eukaryotes - 1604 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR