K10021

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K10021

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K10021

Determination of introduced cDNA(s)

  • J033094J24 - Rice Full-length cDNAs - Original: J033094J24

    full reading [+] show sequence

    Nucleotide sequence: J033094J24 - full reading
    GGGGCTTGTC CTTTGGAAAA TTTCTGGAAC TTAGCTTCTC AAATCATGCG 
    GCAGCTAGTA GGGTAGCCAG TTGTGGCCAT TCTCTTCACA GGGATTGCCT
    TCGTTTTTAC GGCTTTGGTG AAATGGTTGC TTGCTTCCGA TATGCATCGA
    TCAAGGTTCA TTCAGTGTAT TTGCCACCAC CGAAACTTGA TTTCACTTCT
    CAGCACCAGG AATGGGTAGA GCAAGAAGCA AATGAGGTGG TTGACTCAGC
    TGAACTTCTG TTCACTGAAG TACTGAATGC ACTCCACCAA ATTTCAGAGG
    GAAGGCCAAT TACAGGTTCT TTTGATGGCA ACATGAAAAT CCTCGAGTTA
    AGAAGGAACA TCATGGAGCT GGAAGAGATC CTGCAAATAG AGAAAGCAGA
    TTTTACGGAA TCATTGAAGA ATCTACTGAA CAAGGAGATC AGAAAGGGGA
    AGCCATTCAT TGATATTCTT GAGGTTAACA AACTAAGGAG GCAGCTACTG
    TTTCTGTGTT ACCTGTGGGA TCAGCGGCTA ATCTTTATAG CAAATTCAGG
    TGGCAAGTAC TGTGACACAC TAGGAGGTTT ACGGGTTGGG AGTAGGAGTT
    CTGACTCTAA CGATAAATCA GTTGATACCA ATGCAACTAC AAAACTAGAG
    AAGAGCTCAA AGGGCTCCGA AGTTCTTTCA AATGCTAAGG AAGGATCCTT
    GAAGCAGAGC CAAAGTCCAC TTCATGCTAA TGACAAGGAG CCCAACCAAC
    CTGATCAATC TAATGGAAAT AGTTCAAGGA TTGGTGCAGG GTTGAATGGC
    ATGGAAGATG CGATTGCCAA AATCAATCAT TCTAACAGCG CAGATGTTAA
    GGATAACCTG GATCATCAAG AATCCAGTAT TGGTGTTCGT AGGGTTCTTT
    CTGATGGTCA GTTTCCAGTA AATGCTGATA TTTCAGACAC ATTGGATGCA
    AAATGGAGAG GTGAAAATGG AACAGTTCCT GATACAAGTA TCCTGAAACC
    ATTAGCTCTG CTAGAAGGTT CAGCTGATCT TAAAAACCAA GCTAAGGCAG
    TGCCAACCAA TGCTTCTTTA TCTGTCAGGT CTGGAGATAC AGTAGAAGAC
    TTATCAAGCT GGCTCAAAAT GCCATACATG ACATTCTATG ACTCCTTAAA
    CACAAATTCT GGGACAGCAC CGAGATTTGG GGCCCTGGCT GACTACAATC
    CTGTGTACAT TACATTGTTC CGTGAGTTAT CACAGCAAGG TGGAGCAAGA
    CTTTTTCTGC CAACTGGAGC TAATGATGTT GTTATTCCAG TATTTGATGA
    TGAACCGACC AGTATAATTT CTTATGCACT TGTTTCACCC ATGTACTGTC
    ACCAAATGTT GGATGAGAAC AGCAAGAACA AAGATGGTCC AGATTCTTCA
    CTTCCATTGC CTGTATATGA TTCTGGAAAT TTTAATCCTT TTCATTTATT
    TGAGGATTTC GGATCCGCTG ATGATCTTGC TTCGTCAATA TCTGGAACCA
    GAGGGTCATT TGTTCCCGAT CTAGTTCACC TAAGAGTTCC CTTTGAGGAT
    GGTGGCCCAC TCGGGAAGGT GAAATACACA GTGACGTGCT ACTATGCGAA
    AAGCTTTGAA GCTCTTAGAA GGTCTTGCTG TCCATCTGAA CTTGACTTTT
    TAAGATCTAT CAGTCGATGC AAGAAATGGG GAGCACAAGG TGGCAAAAGC
    AATGTGTTTT TTGCAAAATC TCTGGATGAT AGATTTATAA TAAAGCAGGT
    CACAAAAACA GAACTTGAAT CATTCCTTAA GTTTGGACTA GATTATTTTA
    AATATTTATC AGAGTCTATA AGCACCGGAA GCCCTACATC CCTTGCAAAG
    ATTCTGGGAA TCTATCAGGT TACAATTAAG CATGTGAAGG GTGGCAAGGA
    GTCAAAAATG GATTTACTTG TGATGGAAAA TCTTTTATTT GGACGGAATA
    TTACAAGGCT CTATGATCTC AAGGGATCTT CGAGGTCAAG GTATAATGCT
    GATTCCAGCA GCAACAAGGT TCTTCTTGAC CAGAACTTGA TTGAGGCAAT
    GCCAACGTCA CCAATTTTCG TTGGGAACAA GGGAAAGAGG CTTCTGGAAA
    GAGCTGTTTG GAATGATACT TCCTTTCTGG CAGGAATTGA TGTGATGGAT
    TACTCATTGC TTGTGGGAGT TGACGAGGAG AAACATGAAT TGGTTCTGGG
    AATAATTGAT TTTATGAGGC AGTATACATG GGACAAGCAC CTCGAGACAT
    GGGTCAAATC GTCCGGTATT CTCGGCGGGC CGAAGAATGC CCCTCCAACT
    GTGATTTCAC CCATGCAGTA TAAGAAGCGG TTTAGAAAAG CTATGTCTGC
    TTATTTTATT GTAATTCCGG AGCAATGGAT GCCTGCAATA ATCAATCCGA
    GCAAATCATC ATCAAATCTT TGCCAAGAAG ATCCTCAAAA TGCTTCACAA
    GAGTAGTACA ATATTCAGTT CATTATGCCA GGCAGAGAGC ACCTGGTTTT
    TTGTCTTGAT CCAATCAAAA GCGCCAAACG ATCAGTCTGG ATGCTGCGGC
    ATCAATGTGG TATTACGACT GCAATTATCA AAATGCTTGG ACTCTCATTT
    GCACAGATCC GATTGCAGTG GAGTCGTCTT GGCTCAAGAA TCATCGGGTG
    TATATATCCT TGAAACTGTA ATTATGCATT AGTTTTTTTA TTTTCTTTTT
    GATTTATTAT GTAATAATAT AATGTCGTCG CTTCTCTCTA GTTCCCATTC
    CATTTTGGTT GGATGTAAAC AATAGAAGTG GGGCATTTTT TTTTTGTAAG
    TGCTCGTTGT ATATTATTAT ATATATATGC CATGTTATTT

    Gene models with high sequence identity

    1. AT3G14270.1 [+] show detail - phosphatidylinositol-4-phosphate 5-kinase family protein
      E-value: 0; Score: 775.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup 3.4e-118
    Gene3D no description 8.7e-20
    Gene3D no description 2.2e-36
    ProfileScan Phosphatidylinositol-4-phosphate 5-kinase, core 55.573
    HMMPfam Phosphatidylinositol-4-phosphate 5-kinase, core 3.3e-64
    HMMPanther Phosphatidylinositol-4-phosphate 5-kinase, core 1.4e-25
    Coil coiled-coil 0
    superfamily SAICAR synthase-like 5.8e-75

    See the detailed result >

    AT3G14270.1

    Model type
    Protein coding
    Short Description
    phosphatidylinositol-4-phosphate 5-kinase family protein
    Curator Summary
    Encodes a protein that is predicted to act as a 1-phosphatidylinositol-3-phosphate (PtdIns3P) 5-kinase based on its homology to Fab1 from yeast. It contains an FYVE domain required for binding to PtdIns3P-containing membranes in yeast, as well as a Cpn60_TCP1 homology domain plus a kinase domain. fab1a/fab1b pollen grains not viable and have defective vacuolar organization.
    Computational Description
    FORMS APLOID AND BINUCLEATE CELLS 1B (FAB1B); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases (TAIR:AT4G33240.2); Has 8765 Blast hits to 8134 proteins in 458 species: Archae - 587; Bacteria - 13; Metazoa - 3836; Fungi - 1744; Plants - 978; Viruses - 3; Other Eukaryotes - 1604 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR