K10528

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K10528

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K10528

Determination of introduced cDNA(s)

  • J033061I11 - Rice Full-length cDNAs - Original: J033061I11

    full reading [+] show sequence

    Nucleotide sequence: J033061I11 - full reading 
    GAGTGTGCTT GCTCTCTCTC CCATCGCTTT CCCCTCCCCT CGCTTGCCGC 
    CGCATCCATC CACCACCTCT CCCCCTCGCG GATGCGATTC CCTCCCGCCG 
    CCGCCGCCGC CGCCGCCGCC GCCGCGACTA GCTAGCTAGC TCGGAGGTCG 
    GAGGGGAGGG GTGGTACGAC TACAATGGCG AGCGCGTCGG AGGACGACGC 
    CGGCTCGGAG CGGTGCTGCG GGAGCTACAG CCCGAGCGCG GACGTCAGCG 
    AGTCGGAGAC ATCCAGCGAC TGCTCCGCGC CGACGACCAC CACCACCACC 
    CGCCGCTTCG CCTCCTCCCC CTCCCGCGGC GTCGCCTCCT CCTCCTCCTC 
    CTCGCTCCTC CCGACCCCTC CCCCTTCCTC CGCCGCCGCC TTCTTCCTCT 
    CCGCCAAGCC CGCCGCCGAC CTCTCCGAGG TGGACATGAT GAAGGAGCGC 
    TTCGCCAAGC TGCTGCTCGG CGAGGACATG TCCGGGAGCG GCAAGGGCGT 
    CTGCACCGCG CTCGCCATCT CCAACGCCAT CACCAACCTC TCCGCCACTG 
    TGTTCGGCGA GCTGTGGAGG CTGGAGCCGA TGGCTTCGGC GAGGAAGGCG 
    ATGTGGACGA GGGAGATGGA CTGGCTGCTC TCCGTGGCCG ACTCCATCGT 
    CGAGCTCACC CCGTCAATCC AGGAGCTCCC CGACGGCGGG GGACAGTTCG 
    AGGTCATGGT GCCGCGCCCC CGCAGCGACC TCTACATGAA CCTCCCGGCG 
    CTCAAGAAGC TCGATGCGAT GCTCCTCGCC ATGATTGACG GGTTCAAGGA 
    GACCGAGTTC TGGTATGTGG ATAGGGGGAT CGTGGTTGAT GACAGCGGCG 
    GGCCGTTCTC GTCGTCGTCA TCCTCCTGCG GGCGGCCGTC GGTGCGGCAG 
    GAGGAGAAAT GGTGGCTGCC ATGCCCGCGG GTGCCGCCCA AGGGGTTGTC 
    AGAGGATGCG AGGAGGAAGC TGCAACAGGA CAGGGATTGT GCGAACCAGA 
    TACTGAAGGC GGCCATGGCG ATTAACAGCG ATGTGCTCGC GGAGATGGAG 
    ATCCCGGAAG TGTACTTGGA GTCACTGCCA AAGAGTGGTA AATCTTGCTT 
    GGGTGAGATA ATTTACCGAT ATATAACAGC TGAACAATTC TCACCGGAGT 
    GCCTCCTCGA TTGCTTGGAT CTGTCATCAG AACACCACAC ACTTGAAGTA 
    GCTAACAGAA TAGAGGCTGC CATCCATGTG TAGAGGTTGA AGGGCCAAAA 
    GAAATCCACG CCTCAAGCAA AATCAAAGAA ATCTTGGGGT GGAAAAGTGA 
    AGGGACTTGT TGGAGATACA GAAAAGAGTC ATGTTTTGTC CCAAAGAGCT 
    GATGGGTTGT TGCAAAGCTT AAGATTGCGA TACCCTGGTT TGCCACAAAC 
    CTCTCTCGAC ATGAACAAGA TCCAGTATAA CAAGGACGTT GGGCAGTCCA 
    TACTAGAGAG TTACTCAAGG GTTCTGGAGA GCTTGGCATT TAACATAATT 
    GCCAGGATTG ATGATGTAAT TTATGTAGAT GATGCAACAA AGAAGTCTGC 
    CGCTGCTGAC TCTGTTTCCA TCTTCAATCG TGGCATTGGC GTACCAGTTC 
    AGAAGAGGAT CTCCCCAAGC CCGTTCTCAA TTCAGCATAC ACCATATGCC 
    TCTCCATTTG CTACACCCAC TTTCTGCTCC TCCACTCCAG TTACAGGCAG 
    CCCTGGAAGA GTACAGCCTC CATTGAACAA AGACAACTTA CCGACAAAAC 
    AAGAGGTTAA AGTTGAGAAG CTGTTTTCTG GTGATATCGA GAAGGTCTGG 
    ACGTATGCCG GGAACCTTAG CGCAAGGAAA GACGCAGGAG ATGCCCCTGA 
    AAGAGACTGA AAGGCAGTGA AGAAAGTTCT TCAGATCTTT AGACGTGTCT 
    CAGCAAGGTC AACAGGTTTC AACACATCCT GTTCTGGTCT CTAGCTAGAT 
    TTTCCCTGTA CAGAAGCAAC ATGATTCATT TTTTTGTGCT TTCTTAGCGA 
    GCCAAGGGTC GTCTAGCCCT ACTTACAACA AGTAAGGGAA TGACTCTTTG 
    AGTCTTGATC GCATCTGAAC AAGTCATAGT TTCACGTAGC TGCCTCTGTC 
    GGACATGTAC TCTTCTTGTT CATGTTTATA CTAGCTGGTT TAGTTAGCAG 
    CAAGTGATTG AACTTCAGAT GCTGCAGTTC TTTTTTTTTT TTTTTTTGTC 
    CCTGGAGGCA TCGGTTCCCT AACAATCAAC TGCACTTGCA GTGCAATGTA 
    TCATGATTTT CTGATGCTGT TGTATATGGC ACCTAATGGA CAGATCTGAC 
    GCTCTTCAGT GACTCTGAAG ACTTTCTTAT ATC

    Gene models with high sequence identity

    1. AT4G38430.1 [+] show detail - rho guanyl-nucleotide exchange factor 1
      E-value: 5e-153; Score: 538.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Rop nucleotide exchanger, PRONE 8.9e-138
    HMMPfam Rop nucleotide exchanger, PRONE 8.5e-42
    FPrintScan PRICHEXTENSN 8.6e-10
    FPrintScan PRICHEXTENSN 8.6e-10
    FPrintScan PRICHEXTENSN 8.6e-10
    ProfileScan Rop nucleotide exchanger, PRONE 91.674
    ProfileScan Rop nucleotide exchanger, PRONE 30.302

    See the detailed result >

    AT4G38430.1

    Model type
    Protein coding
    Short Description
    rho guanyl-nucleotide exchange factor 1
    Curator Summary
    Member of the RopGEF (guanine nucleotide exchange factor) family, containing the novel PRONE domain (plant-specific Rop nucleotide exchanger), which is exclusively active towards members of the Rop subfamily, also known as DUF315). Interacts with ROP1 but the whole protein lacks Rho guanyl-nucleotide exchange factor activity in vitro. The DUF315/PRONE domain is sufficient to confer RopGEF catalytic activity.
    Computational Description
    rho guanyl-nucleotide exchange factor 1 (ROPGEF1); CONTAINS InterPro DOMAIN/s: Rop nucleotide exchanger, PRONE (InterPro:IPR005512); BEST Arabidopsis thaliana protein match is: RHO guanyl-nucleotide exchange factor 7 (TAIR:AT5G02010.1); Has 308 Blast hits to 304 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 308; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR