K11625

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K11625

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
petal (PO:0009032)
  • abnormal (PATO:0000460)
    (Flower::Shape::petal)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K11625

Determination of introduced cDNA(s)

  • J033070A11 - Rice Full-length cDNAs - Original: J033070A11

    full reading [+] show sequence

    Nucleotide sequence: J033070A11 - full reading 
    GGTTCATTTT CCCCTCTTCC CCCGCTCCGC TCTCGTCTCC GCTCTGCTCT 
    GGTCTGCTCT GCTGCGAGGG TGGGTGTTGC TTGTACCGGG CGGGGATGGA 
    GGCGGCGACG ATGGCGTGGA CGGCGGCGGG GGTGGGGATG GCGCTGGTGT 
    ACTGGTTCGT GTGGGTGATG GGAGCGGCGG AGGTGAAGGG GAAGCGGGCG 
    GTGGATCTCA AGATGGGATC CATCACGAAC GACAAGGTGA AGGACAAGTA 
    CACGCAGTAC TGGTCGTTCT TCCGCCGCCC CAAGGAGACG GCGACCACCG 
    AGGCCTCCGC CGAGAAGGTG CCGGCCTTCG TCGACACCTT CTACAACCTC 
    GTCACCGACA TCTACGAGTG GGGTTGGGGT CAGTCGTTCC ACTTCTCCCC 
    TTCCCTCCCC GGCCGCTCCC ACCGCGAGGC CACGCGCGTC CACGAGGAGC 
    GCGTCGCCGA CCTCCTCCAG GCCAAGCCGG GACACCGCCT CCTCGACGTC 
    GGCTGCGGCG TCGGCGGGCC CATGCGCGCC ATCGCCGCCC ACTCCGGCTC 
    CAACGTCGTC GGCATCACCA TCAACGAGTA CCAGGTGAAC CGCGCCCGCG 
    CGCACAACCG CAAGGCCGGC CTCGACTCCC GCTGCGAGGT CGTCTGCGGT 
    AACTTCCTCT CCATGCCGTT CTCCGACGCC TCCTTCGACG GCGCCTACTC 
    CATCGAGGCC ACCTGCCACG CGCCCAGGCT GCAGGACGTC TACGGCGAGG 
    TCTTCCGCGT GCTCAAGCCG GGCGGCCTCT ACGTCTCCTA CGAGTGGGTC 
    ACCACCTCCC TCTACCGCGC CGACAACCCG GAGCACGTCG AGGCCATCCA 
    CGGCATCGAG CGCGGCGACG CGCTCCCGGG TCTCCGCCGC CAGGACGAGA 
    TCGCGTCCAT CGCCAAGGAG GTCGGATTCG AGGTGCTCAA GGAGCTCGAC 
    CTCGCCCTCC CGCCCGCTCT CCCATGGTGG ACTCGCCTCA AGATGGGGCG 
    CATCGCATAC TGGCGCAACT CCCTCGTCGT CCGCGTGCTC ACCATGCTCC 
    GGATTGCACC CAAGGGCGTC TGCGAGGTGC ACGAGATGCT CTACGAGACC 
    GCGCAGCACC TCACCCGCGG CGGCGAGACC GGCATCTTCA CGCCGATGCA 
    CATGGTGCTC CTCCGCAAGC CCGTCGAGAG CAAATAGTGC AACAAAGTCA 
    TCGACGACAA CAACAACACC TACCTTCATT CACTCACCAG CGGAAGAAAC 
    AGAGGGAAAA CAACAAAAAA AATCTCGCAG ATCAGCAGCT GAGGTAAGAA 
    GAGCAGACTA CCTACCTGGT GAGGAGGAGA GGTTGAAGAA GAGAGTGCCT 
    TAAAATTAAG TTAAATTTGA TCGTTTAGAG GAGAAATAAG GATGATGTTT 
    ATTGTTACTC TTTCTTGTTC GTAGCTAATT TTGCTTTCAA TTTTTTTGGT 
    GGAGGATTTT GGGTTGGAAA GACAAGGTCG GGATCTCTTT TAAAGTTTGT 
    TTTTCTTTTC TTTTCCCTCT TGATCGGTGG ATCGATCTAC TATCAGTATC 
    AGTAATCAGC CATGCCATGT TTTGTCGAGC TCACAACATT GGAATCTCCC 
    GCTAGGGAAT CAATTTTCAA CTGTAATTTG CCCCGCTTTC TGCGGTGAGA 
    TTTGCAGGTG CGCAATATGT AATTCACCAT TTTCATTGCT CATCGCTCCA 
    GTGCAAGAAA AAGGTTGGAA ACGACAAACA TTCTGACC

    Gene models with high sequence identity

    1. AT1G20330.1 [+] show detail - sterol methyltransferase 2
      E-value: 7e-148; Score: 521.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther S-ADENOSYL-L-METHIONINE:DELTA24-STEROL-C-METH 1.2e-187
    HMMPanther METHYLTRANSFERASE 1.2e-187
    superfamily S-adenosyl-L-methionine-dependent methyltrans 2.3e-59
    Gene3D no description 1.9e-48
    HMMPfam Methyltransferase type 11 2.4e-21
    HMMPfam Sterol methyltransferase C-terminal 7.3e-14

    See the detailed result >

    AT1G20330.1

    Model type
    Protein coding
    Short Description
    sterol methyltransferase 2
    Curator Summary
    Encodes a sterol-C24-methyltransferases involved in sterol biosynthesis. Mutants display altered sterol composition, serrated petals and sepals and altered cotyledon vascular patterning as well as ectopic endoreduplication. This suggests that suppression of endoreduplication is important for petal morphogenesis and that normal sterol composition is required for this suppression.
    Computational Description
    sterol methyltransferase 2 (SMT2); FUNCTIONS IN: S-adenosylmethionine-dependent methyltransferase activity; INVOLVED IN: xylem and phloem pattern formation, negative regulation of DNA endoreduplication, multidimensional cell growth, sterol biosynthetic process, pattern specification process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sterol methyltransferase C-terminal (InterPro:IPR013705), Methyltransferase type 11 (InterPro:IPR013216); BEST Arabidopsis thaliana protein match is: sterol methyltransferase 3 (TAIR:AT1G76090.1); Has 13231 Blast hits to 13223 proteins in 2296 species: Archae - 440; Bacteria - 9200; Metazoa - 162; Fungi - 455; Plants - 664; Viruses - 0; Other Eukaryotes - 2310 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR