K12727

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K12727

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K12727

Determination of introduced cDNA(s)

  • J033075P18 - Rice Full-length cDNAs - Original: J033075P18

    full reading [+] show sequence

    Nucleotide sequence: J033075P18 - full reading 
    GAGCAAGAGC CGATCGATCA TTGTCCAAGT CTCCAAGCGA TCAGCTAGCT 
    GACTTGACGA CGCTGCGAGC AGCGGCAGCA AGAGAGCAGC AGCACAAAGG 
    GTTCCCCTTT CACAAGTGTC CGGCCGGCTG CACGTAGGCC ATGGGGAAGC 
    AAATGGCCGC CCTGTGTGGC TTTCTCCTCG TGGCGTTGCT CTGGCTCACG 
    CCCGACGTCG CGCACGCGCA GACGCAGATC CTCTTCCAGG GTTTTAACTG 
    GGACTCGTGG AAGAAGCAGG GTGGTTGGTA CAACATGCTC AAGGACCAGG 
    TGGGCGACAT CGCCAGCGCC GGCGTCACGC ACGTCTGGCT CCCACCTCCC 
    ACGCACTCCG TCTCGCCTCA AGGCTACATG CCGGGACGGC TCTACGACCT 
    GAACGCGTCC AAGTACGGCA CCAAGGCGGA GCTCAAGTCG CTCATCGCCG 
    CCTTCCACGC CAAGGGCATC AAGTGCGTCG CGGACATCGT CGTCAACCAC 
    CGCTGCGCCG ACGACAAGGA CGGCCGCGGC GTCTACTGCA TCTTCAAGGG 
    CGGCGGCCCG CGCGGCTGCC TGGACTGGGG CCCCTCCATG ATCTGCTGCG 
    ACGACACGCA GTACTCCGAC GGCACGGGCC ACCGCGACAC CGGCGCCGAC 
    TTCGCCGCGG CGCCCGATAT CGACCACCTC AACCCGCTCG TCCAGCGGGA 
    GCTCTCCGAC TGGCTCAGGT GGCTCAGGAG GGATGTCGGC TTCGACGGCT 
    GGCGCCTCGA CTTCGCCAAG GGCTACTCGG CGGCCGTCGC CAGGACGTAT 
    GTCCAGAACG CCAGGCCGAG CTTCGTCGTG GCGGAGATAT GGAACTCCCT 
    GAGCTACGAC GGCGACGGGA AGCCGGCGGC CAACCAGGAC GGCCAGCGGC 
    AGGAGCTCGT GAACTGGGTG AAGCAGGTTG GCGGCCCGGC GACGGCGTTC 
    GACTTCACGA CCAAGGGCAT CCTGCAGTCG GCGGTGCAGG GCGAGCTGTG 
    GCGGATGCGG GACAAGGACG GCAAGGCGCC GGGCATGATA GGCTGGTATC 
    CCGAGAAGGC CGTCACCTTC GTCGACAACC ACGACACCGG CTCGACGCAG 
    CGGATGTGGC CATTCCCGTC TGACAAGGTC ATTCTGGGCT ACGCCTACAT 
    CCTCACCCAC CCAGGAGTAC CATGCATCTT CTACGACCAC GTGTTCGACT 
    GGAACCTCAA GCAAGAGATC AACGCGCTGG CGGCGACGAG GAAGCGCAAC 
    GGGATAAACG CCGGGAGCAA GCTGCGCGTC CTGGCGGCGG AGAGCGACAT 
    GTACGTGGCG ATGGTCGACG AGAGGGTCAT CACCAAGATC GGGCCGAGAA 
    TAGACGTGGG CAACATAATC CCGTCGGACT TCCACATCGT GGCTCATGGC 
    AATGACTACT GCGTCTGGGA GAAGAGCGGC CTTAGAGTCC CAGAACCAGA 
    AGGTCGGCGC TAGAAACGGC TCGCAAGCTA GCTACACAGT TCTTAATAAC 
    TTGGTCGCAA AAAAAAAGTT CTTAATAATT TGTACGAGAA ATGTTCGTTT 
    CTGTTTGCAT ATATAGCTTA TGAAATCTTA CC

    Gene models with high sequence identity

    1. AT4G25000.1 [+] show detail - alpha-amylase-like
      E-value: 9e-145; Score: 510.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Glycoside hydrolase, catalytic core 2.4e-89
    HMMPfam Glycosyl hydrolase, family 13, catalytic domain 1.3e-27
    HMMPfam Alpha-amylase, C-terminal beta-sheet 1.2e-21
    HMMSmart Glycosyl hydrolase, family 13, subfamily, catalytic domain 1.3e-64
    HMMSmart Alpha-amylase, C-terminal beta-sheet 5.4e-38
    FPrintScan Glycoside hydrolase family 13 1.4e-07
    FPrintScan Glycoside hydrolase family 13 1.4e-07
    FPrintScan Glycoside hydrolase family 13 1.4e-07
    Gene3D Glycoside hydrolase, subgroup, catalytic core 1.4e-103
    HMMPanther ALPHA-AMYLASE 3.4e-206
    HMMPanther AMYLASE 3.4e-206
    HMMPIR Alpha-amylase, plant 0

    See the detailed result >

    AT4G25000.1

    Model type
    Protein coding
    Short Description
    alpha-amylase-like
    Curator Summary
    Predicted to be secreted protein based on signalP prediction. Involved in starch mobilization. Mutants are defective in alpha-amylase activity. (Note: AMY1 has been found in the literature to be referred to as AMY3, which is not to be confused with AMY3/At1g69830).
    Computational Description
    alpha-amylase-like (AMY1); FUNCTIONS IN: alpha-amylase activity; INVOLVED IN: response to gibberellin stimulus, response to abscisic acid stimulus; LOCATED IN: extracellular region, apoplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 13 (InterPro:IPR006046), Alpha-amylase, plant (InterPro:IPR013775), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047), Alpha-amylase, C-terminal beta-sheet (InterPro:IPR012850); BEST Arabidopsis thaliana protein match is: alpha-amylase-like 2 (TAIR:AT1G76130.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR