K12802

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K12802

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K12802

Determination of introduced cDNA(s)

  • J013050D15 - Rice Full-length cDNAs - Original: J013050D15

    full reading [+] show sequence

    Nucleotide sequence: J013050D15 - full reading 
    GACCACCCGT GAAGCTCTGG AGTCGAGCGT GAACTTGGTC CACTACGCCA 
    TGGCGGCCGC ACCACAGCAA TCCGTGCACC CAAGCTTGCC CTCCTCAACC 
    TCAACCCTCC GCCTCCTCAT CTCCTCCTCC CCACGACGGC CTCCTCCGCC 
    GCCGCCAAGA GCCCGCCGGT ATAACAGGCT CGCGGCGTCG GCTTCGGCGG 
    CGCGGGAGAT GCCGTGGCCG CACGTGCTGA CGGTGGCCGG CTCCGACTCC 
    GGCGGCGGCG CCGGCATCCA GGCCGACATC AAGGCCTGCG CCGCGCTGGG 
    AGCCTACTGC TCCTCCGTCG TCACCGCCGT CACTGCCCAG AACACCGCCG 
    GCGTCCAGGG GATTCATGTG GTGCCAGAGG AGTTCATCCG GGAGCAGCTC 
    AACTCGGTTC TTTCGGACAT GTCGGTGGAT GTGGTCAAGA CAGGAATGCT 
    CCCTTCAATC GGAGTAGTCA GAGTTTTATG TGAAAGTCTA AAGAAGTTTC 
    CAGTCAAAGC TTTAGTGGTG GATCCAGTTA TGGTGTCCAC AAGTGGAGAT 
    ACTCTTTCAG AATCATCTAC TCTCTCTGTA TATAGGGATG AATTATTTGC 
    TATGGCTGAT ATAGTCACCC CAAATGTGAA AGAAGCGTCG AGATTACTTG 
    GGGGTGTATC TTTGCGCACT GTTTCTGACA TGCGCAATGC AGCGGAGTCC 
    ATTTACAAAT TTGGTCCAAA ACATGTACTT GTGAAAGGTG GGGATATGCT 
    AGAATCCTCA GATGCAACTG ATGTATTTTT TGATGGTAAG GAGTTCATTG 
    AGCTCCATGC GCATCGCATA AAGACCCATA ACACACATGG AACTGGTTGC 
    ACTTTAGCTT CATGTATAGC TTCCGAATTA GCGAAAGGTG CAACGATGCT 
    GCATGCTGTT CAGGTGGCTA AGAATTTTGT AGAATCTGCT CTTCATCACA 
    GTAAAGATCT TGTCGTTGGA AATGGACCTC AAGGCCCTTT TGATCACCTT 
    TTCAAGCTCA AGTGTCCGCC ATACAATGTT GGCTCACAGC CAAGCTTTAA 
    GCCAGACCAA CTCTTCCTGT ATGCAGTGAC AGACTCTGGG ATGAACAAAA 
    AGTGGGGTCG TTCAATCAAA GAAGCTGTGC AAGCTGCCAT TGAAGGTGGC 
    GCTACCATTG TACAACTGAG AGAAAAAGAT TCCGAAACAA GAGAGTTTTT 
    GGAAGCAGCC AAAGCATGCA TGGAGATTTG CAAGTCCAGT GGAGTACCAC 
    TGCTGATCAA CGACCGGGTA GACATTGCCC TGGCATGCAA TGCAGATGGT 
    GTCCATGTTG GTCAATTGGA CATGTCGGCA CACGAAGTGC GGGAGCTCCT 
    AGGGCCGGGT AAAATCATCG GCGTCTCGTG CAAGACCCCT GCTCAAGCCC 
    AGCAGGCCTG GAATGATGGA GCAGACTACA TTGGCTGTGG CGGTGTTTTC 
    CCTACCTCCA CGAAGGCGAA CAATCCTACA TTGGGGTTTG ATGGCTTGAA 
    GACAGTTTGC CTGGCATCTA AGCTGCCTGT GGTCGCTATT GGCGGCATCA 
    ACGCTTCAAA TGCAGGTTCA GTGATGGAGC TTGGTCTCCC AAACCTCAAA 
    GGCGTCGCAG TAGTCTCCGC TCTGTTTGAC CGTCCGAGTG TCGTAGCCGA 
    AACAAGAAAC ATGAAATCCA TCTTGACCAA CACCTCTAGA ACCTAGAGCT 
    ACTACAATAT TCTTTGTTCG AAGGGGATAT GTTTATCAAA ATGAAGAGAT 
    GTTTCCAACT TTAATAATCA GAAGGCATTT CTTGTAATAA TGCTTGATCA 
    AAAGCATCTG TGATGCAGGG GAATGAGGAA TCACAATCTT GCATCATTGG 
    GATGTAAGGT ACAATGTTCA GATTTCTTTT

    Gene models with high sequence identity

    1. AT1G22940.1 [+] show detail - thiamin biosynthesis protein, putative
      E-value: 0; Score: 667.00

     

    InterPro Scan Digest

    Program Description E-value
    HAMAP Thiamine monophosphate synthase 27.381
    Gene3D no description 5.4e-86
    Gene3D Aldolase-type TIM barrel 4.2e-60
    HMMPanther PHOSPHOMETHYLPYRIMIDINE KINASE 4.2e-167
    HMMPanther PHOSPHOMETHYLPYRIMIDINE KINASE 4.2e-167
    superfamily Ribokinase-like 4.9e-74
    superfamily Thiamine monophosphate synthase 5.8e-61
    HMMTigr Phosphomethylpyrimidine kinase type-2 1.8e-134
    HMMTigr Thiamine monophosphate synthase 7.5e-79
    HMMPfam Phosphomethylpyrimidine kinase type-1 4e-89
    HMMPfam Thiamine monophosphate synthase 1.1e-64
    HMMPfam Ankyrin repeat 5.1e-05

    See the detailed result >

    AT1G22940.1

    Model type
    Protein coding
    Short Description
    thiamin biosynthesis protein, putative
    Curator Summary
    Encodes a bifunctional enzyme required for thiamine (vitamin B1) biosynthesis. TH1 can phosphorylate HMP-P to produce HMP-PP, the pyrimidine heterocyclic subunit of thiamine. TH1 also catalyzes the condensation of HMP-PP and HET to form thiamine monophosphate (TMP). TH1 also appears capable of phosphorylating HMP based on E.coli mutant complementation assays. th1 mutants are thiamine auxotrophs that die as seedlings on unsupplemented media.
    Computational Description
    THIAMINE REQUIRING 1 (TH1); FUNCTIONS IN: phosphomethylpyrimidine kinase activity, thiamin-phosphate diphosphorylase activity, hydroxymethylpyrimidine kinase activity; INVOLVED IN: thiamin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine monophosphate synthase (InterPro:IPR003733), Phosphomethylpyrimidine kinase type-2 (InterPro:IPR004399), Aldolase-type TIM barrel (InterPro:IPR013785), Phosphomethylpyrimidine kinase type-1 (InterPro:IPR013749); Has 15989 Blast hits to 15891 proteins in 2548 species: Archae - 338; Bacteria - 12149; Metazoa - 165; Fungi - 331; Plants - 107; Viruses - 0; Other Eukaryotes - 2899 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR