K12945

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K12945

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K12945

Determination of introduced cDNA(s)

  • J023134L09 - Rice Full-length cDNAs - Original: J023134L09

    full reading [+] show sequence

    Nucleotide sequence: J023134L09 - full reading
    GAAAGGAACT TCTTTTCTGA AGACTGATTA AAAAAACATG ACCTTTGAAT 
    CAAGCTTGTG GCCTCTCAGA TAACGAACTT TTCCTGCCAC CTAGTTATGA
    GTATAATATG GCTTACATTT ACACAGGTGA AAATGAAAAT GAGAAAAGAA
    AAGGCACAAT AAGAAACAAC GGCTGATGAT TTATTATGTT CTGCCCTTCT
    TTGTTGTGAC AAAGTATCTT GATCACAAGG ACGGAATTGG CAGGGTAGCT
    GGCAGTTGCA GTAGCATTAA CTGCCAACTG TGTGCTGCTA ATCTTATTTG
    ATCCAAATTA TATGGTTATT AATCAAATCC CATAGCCCCA TAATCCCCAA
    CCATGCCCGA AGAAGCTGCT GGGATGGCGT CAGTCTTGCG GAGGCTCTAC
    CTCTCTGTCT ACAACTGGAT TGTCTTCATC GGATGGGTTC AGGTGTCGTG
    GTCCATGATA TTGGCATTGT TGGAGAAGAG ATATGAGGCC GTCTACGCTG
    CTGTAGAGCA GCATCTGCTG TTCGCGCAGA CTGCTGCCAT CATGGAGATT
    CTTCATTCCA TTGTAGGGTT GGTGAGGTCT CCAGTGTCTT CTACTCTTCC
    ACAAATTACT GCAAGGTTGT TCATGATCTG GGGCATCTTG CGGAGCTTCC
    CTGAGATACA CACTCATATT TTTGTTACCT CCTTGCTCAT AAGCTGGTGC
    ATCACTGAGG TTACCAGATA TTCTTTCAAT GGCATGAAGG AGTCATTTGG
    ATTTACACCT TCCTGGCTCT TATGGCTTAG GTACAGCACC TTCATAGTAT
    GCTTTCCTAT TGGTATGGTT AGTGAGGTTG GCCTAGTCTA CATTGTTGTT
    CCTTTCATGA AGGCATCTGA GAAATACTGC CTTAGGATGC CCAACAAATG
    GAATTTCTCC ATCAAATACT TCTATGCCTC TGTCTTTTTC ATGGCTTTGT
    ATGCCCCAGT ATACCCGCAC TTGTTCCACT ATTTGATCGT CCAGCGGAAG
    AAGGCCTTGG CAAAATCAAA AACCACATAA CTACTCAAGA GCTATCTAAT
    CTGGTTTCTG CTCGCAGCTA CAGCTCAGGT TGCTACCACT TCTTGAACAA
    AGAAGTAAAA ATGTATTAGA ATGGAAATGC CGAACTACAC AGACTATCTT
    TTCCCTTGGT AAATAAAAAT GGACATGAGT ACTATTTTCA TGATTTGTTA
    ATGGTGTGTT GTACCAAACT TTGGCTTTTC CATATGTAAA TAAAATTGCA
    CTCCATATCG ATGCC

    Gene models with high sequence identity

    1. AT5G10480.1 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
      E-value: 8e-72; Score: 268.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Protein-tyrosine phosphatase-like, PTPLA 3e-53
    HMMPanther PHOSPHATASE RELATED PROTEIN PTPLA 2.5e-59
    HMMPanther Protein-tyrosine phosphatase-like, PTPLA 2.5e-59

    See the detailed result >

    AT5G10480.1

    Model type
    Protein coding
    Short Description
    Protein-tyrosine phosphatase-like, PTPLA
    Curator Summary
    Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
    Computational Description
    PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR