K13823

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K13823

Determination of introduced cDNA(s)

J033106K23 - Rice Full-length cDNAs - Original: J033106K23

full reading [+] show sequence

GGTTTGTGAG GTGAGGTGAG TTGAGTTGGG GATTGATTGA TTGATTCGGA 
TTGGGAAGAA GAAGAAGCAG GGGAGCATGG GCAGCAAGTC GTACCCGACG 
GTGAGCGATG AGTACCTGGC GGCCGTGGGC AAGGCGAAGC GCAAGCTCCG 
CGGCCTCATC GCCGAGAAGA ACTGCGCCCC ACTCATGCTC CGCCTCGCGT 
GGCACTCTGC TGGCACCTTC GATGTGTCGT CGAGGACCGG CGGGCCCTTC 
GGCACCATGA AGAACCCCGG CGAGCAGTCC CAAGCCGCCA ACGCCGGCCT 
CGACATCGCC GTCAGGCTTC TCGACCCCAT CAAGGACCAA CTTCCCACCC 
TCTCCTACGC CGACTTCTAC CAGCTTGCTG GCGTTGTGGC CGTCGAGGTC 
ACCGGCGGAC CTGAGGTCCC CTTCCATCCG GGCAGGCAGG ACAAGCCTGA 
GCCTCCTCCT GAAGGCCGTC TTCCTGATGC CACACAAGGT TCTGACCACC 
TAAGGCAGGT CTTTTCTGCG CAGATGGGTT TGAGTGACAA GGACATAGTT 
GCTCTTTCTG GTGGTCACAC CCTGGGAAGA TGCCACAAGG AGAGATCTGG 
CTTTGAGGGA GCCTGGACGT CCAACCCTTT GATCTTTGAC AACTCTTACT 
TCACCGAGCT TGTGAGTGGC GAGAAGGAAG GCCTTCTTCA GCTGCCAAGT 
GACAAAGCCC TCATGGCTGA CCCAGCCTTC CGTCCACTGG TGGAGAAATA 
TGCTGCGGAT GAGGACGCCT TCTTTGCGGA TTACGCCGAG GCCCACCTCA 
AGCTCTCTGA ACTGGGATTT GCTGAGGAAT AAGAAGCCTT TAGAGAGCGG 
GATATCCGCA AAAGATTAAT GCCGATTTGT ATTTTGCGCC TTAGAGTCAG 
TACGATCAAG ACTGTCGTGG CGGTTGTAAT AAAAATTAGT GTGCTTTGGG 
CCATCTTTTT ATGTGATTCC AATTGTCTTT CTCTTCATTC TTGCTTTGAT 
GCTCTTTGTC TGGACCTCTA GACCGCCGTA TTGTACTGTG GAGTTTCAAA 
GTTACCAAGC TATTTGCTGT CAAGATAACT ATGGATTGAA TTCCCCTTGA 
TGGATGAAAG CTCATTAGGG ATAGCTCCAG TAACGTTGTG TGTCCATCCT 
TGAGCACTCT CAACATGGAA CAATTGCGTA CTTTTGGGGA AATTCTGCAT 
CTGAATTGGT TGCACCAAAT GACCAAAGAT CAAGCACTGC AAATGATGGG 
GTGCTGATGC AGATAGATGC TGGTATCTGT CCTGTAAAGC TGTTGTTACT 
TGCATTCGGA GCAACCAGGT TTTTCATCAT TTCCGTACTG CTGATGGAAA 
TTGTCCTGTG AAAAAAATTG CTGGAGATAT TCATTACCTG TAGTGGAAGG 
GAAAGGCTAG GATTTGATGA CTGCAGCTCT TGAAGTGGAC CATCTAGGTG 
GTTGAAGCTG ACATCAAGGA CAGCGATGCT GCTGAACAAC AACGAATCCA 
TTGGTAGACC ACCGGAGAGA GAATTGTGGG AGAGGTTGAG GTGCAACAAC 
TTGGTGAGAT TGCTAAGGGA TGGAGAGATG CTCCCTGCAA GGCCTTTGGA 
AGGCAGCGAG ATATCTCTGA CTGTGTTTTC ACCTCTGCAG TTGATCCCTT 
CCCATGAACA GCAGTCAGTA CCTAACGCCC ACGACATGTT GAGGCTGCCG 
TTGTGTTCAG GTGAGAGCTG CGCAACGAAG TCGAGGGAGC TCTGCTCCTG 
CTATATGAAG GAACTGGTGG GAGAGGCAAA GGTGAGCAGC AGCAACAGCA 
TAACACAAAG CTCCCAAAAG GCATGGGGGA TTTGTTTCTG AATCTGGACT 
GGAAACACGA GAAATGAAAT CATAGGGAGG CTGTACATAG TGCAAATAAC 
AGAATCTGAA GTTTCATTCT CTGAATGTCA TGATATCCAA CCTGAAAGTC 
AGAGCTAAGA ATGATGTTCA TCTCTAAGTC TAAGACAAAG TCAGAGAATG 
ATGTCCACAC GATGCAAAAA TAATGGAGTG AAATTCCTGG GCAAGTACAG 
TCACGTAGAA CCACCAGTTA GCATTTCCCT GTTATATATA GTAGCATATC 
TCTGATTAGA TGTGTGATTT GCCATACTGA AGTCCGCAGC TTGGACCAAA 
ATTTTGTAGG TAAGGCGAGC CGTATTATGG AGGATTCACT GGAGGTACAA 
TACATGGGAA TTTAC

Gene models with high sequence identity

AT1G07890.8 [+] show detail - ascorbate peroxidase 1
E-value: 2e-105; Score: 380.00

InterPro Scan Digest

Program	Description	E-value
ProfileScan	Haem peroxidase, plant/fungal/bacterial	15.478
HMMPanther	SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE	1.6e-11
superfamily	Haem peroxidase	4.3e-86
superfamily	L domain-like	7.3e-16
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Plant ascorbate peroxidase	2.1e-117
FPrintScan	Haem peroxidase, plant/fungal/bacterial	3e-27
FPrintScan	Haem peroxidase, plant/fungal/bacterial	3e-27
FPrintScan	Haem peroxidase, plant/fungal/bacterial	3e-27
FPrintScan	Haem peroxidase, plant/fungal/bacterial	3e-27
FPrintScan	Haem peroxidase, plant/fungal/bacterial	3e-27
PatternScan	Peroxidases heam-ligand binding site	0
PatternScan	Peroxidase, active site	0
HMMPfam	Haem peroxidase, plant/fungal/bacterial	9.6e-47
HMMPfam	Leucine-rich repeat-containing N-terminal domain, type 2	0.00035
Gene3D	no description	2.8e-41
Gene3D	no description	3.2e-17

See the detailed result >

AT1G07890.8

Model type: Protein coding
Short Description: ascorbate peroxidase 1
Curator Summary: Encodes a cytosolic ascorbate peroxidase APX1. Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1, APX2, APX6), two chloroplastic types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms. At least part of the induction of heat shock proteins during light stress in Arabidopsis is mediated by H2O2 that is scavenged by APX1. Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress.
Computational Description: ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: response to reactive oxygen species, response to salt stress, response to heat, hydrogen peroxide catabolic process, embryo development ending in seed dormancy; LOCATED IN: cytosol, chloroplast, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016), Peroxidase, active site (InterPro:IPR019794); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR