K13935

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K13935

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K13935

Determination of introduced cDNA(s)

  • J033040F19 - Rice Full-length cDNAs - Original: J033040F19

    full reading [+] show sequence

    Nucleotide sequence: J033040F19 - full reading 
    GGCCGCCGCC GCATCGCGCT GGGGCTCTCC GCTCCTCCGC CGCATCATGT 
    TGTGGAGCTC CGCCCCTCCG CCGTTGCCGT CTACCATGGA AGAGCGAGAT 
    GCGCACCCTC AAGATCGCCG CTTGGATGCT CCGTTTCTCC TCCCTGCAGC 
    GCCATGCGCA CCCCCAAGAT TGCCGCGTAG CCGCCACCAG CAGACCTCCC 
    AACCGGCCAT AGAAGAAGGG GATGGATCAG ATGGGCGGAT GCTCCACCTT 
    TCAGCGCCAC ACCCAGCATC ACTCTCGCCG CGCCGCCGCC AACGACGGAC 
    CTCAACATGT GTTGATGCTG CAGAAGGTGG TCAAAGCATG GAGACCCCTC 
    AGATCGTTGT TGGTGCTATA CTATAAAGAA GATATACTGC CTCGAGTTGA 
    TGATTTTGAT ATCTTAACCT GGTGGAAGGC AAACTCACTA AAATATCCTG 
    TACTCTCACA CATAGCACGT GATGTGTTAG CCATTCCAGC ATCAACGGTT 
    CCATCTGAAA ATGCTTTCAG TACAGGAGGG AGGGTAATCA ATGACTACAG 
    AAGTAGATTG ACGCCTGAGA TAGTTGAGGC TCTCATTTGT CTACAGGATT 
    GGATGAGAGT CGTTGATTGC CCTTCTGTTG GGATGGTCTT ATCACAAGAA 
    GAAGAAACTT GATGCCTTGA TAGGTTGTGG AAAATTTCAG TGCTTCCTGG 
    TGTGGACCAT GTCGTGTCAT TGCACCTATT TATGCTGAGA TGTCAAAGAC 
    TTATCCTCAA CTCATGTTCT TGACAATAGA TATGTTGATG ACCTAATGAT 
    AATTGTAACC ATTTGTTTGG TCAAATCTGC TATTGAAAAA TGATTAGTTT 
    C

    Gene models with high sequence identity

    1. AT3G42170.1 [+] show detail - BED zinc finger ;hAT family dimerisation domain
      E-value: 2e-19; Score: 93.60

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam HAT dimerisation 8.6e-32
    HMMPanther BED FINGER-RELATED 8.5e-09

    See the detailed result >

    AT3G42170.1

    Model type
    Protein coding
    Short Description
    BED zinc finger ;hAT family dimerisation domain
    Curator Summary
    transposase-like gene with conserved domains from the family of hAT transposases that includes hobo from Drosophila melanogaster, Activator (Ac) from maize, and Tam3 from snapdragon but lacks several amino acids known to be essential for Ac transposition5. The DAYSLEEPER gene lacks 8 bp duplications and TIRs (a common feature of transcriptionally silent hAT transposases), however, DAYSLEEPER expression was detected, and several expressed sequence tags are available. The expression seems to be under the control of factors determining the circadian rhythm. DAYSLEEPER was isolated as a factor binding to a motif (Kubox1) present in the upstream region of the Arabidopsis DNA repair gene Ku70. Mutant plants lacking DAYSLEEPER or strongly overexpressing this gene do not develop in a normal manner.
    Computational Description
    BED zinc finger ;hAT family dimerisation domain; CONTAINS InterPro DOMAIN/s: HAT dimerisation (InterPro:IPR008906), Zinc finger, BED-type predicted (InterPro:IPR003656); BEST Arabidopsis thaliana protein match is: BED zinc finger ;hAT family dimerisation domain (TAIR:AT1G18560.1); Has 1233 Blast hits to 1128 proteins in 97 species: Archae - 0; Bacteria - 4; Metazoa - 232; Fungi - 219; Plants - 755; Viruses - 0; Other Eukaryotes - 23 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR