K14139

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K14139

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14139

Determination of introduced cDNA(s)

  • J013131C14 - Rice Full-length cDNAs - Original: J013131C14

    full reading [+] show sequence

    Nucleotide sequence: J013131C14 - full reading 
    GTACCCCCCT CGCCGTCGCC GCCGCCGCCG CCGCCGACGC CGCCGCGACC 
    ACCTCTCGCA AGGGGATTTT GTAGGGGTTT CTGATCCGAG GAGCGGGCGA 
    TGTCGGACTC CGAGGAGCAC CACTTCGAGT CGAAGGCCGA CGCCGGCGCC 
    TCCAAGACCT ACCCGCAGCA GGCTGGGACC ATCCGCAAAA ATGGCTACAT 
    AGTCATCAAG AACCGCCCCT GCAAGGTTGT TGAGGTTTCA ACCTCTAAAA 
    CTGGGAAGCA CGGTCACGCC AAGTGCCACT TTGTTGCAAT TGACATCTTT 
    ACTGCCAAGA AGCTCGAAGA TATTGTGCCA TCTTCTCACA ACTGTGATGT 
    CCCGCATGTG AACCGTACGG AGTACCAGCT CATTGATATA TCTGAGGATG 
    GATTTGTGAG TCTGTTAACT GAAAATGGCA ACACCAAGGA TGACCTCAGG 
    CTCCCTACTG ATGATAACCT GCTTAGTCAG ATCAAGGATG GATTTGGAGA 
    AGGGAAGGAC CTGGTGGTGA CTGTTATGTC TGCCATGGGA GAGGAGCAGA 
    TCTGCGCGCT GAAGGACATC GGCCCGAAGT AAACCTCACC TGTTCCATGT 
    GATTACTACT GAACTTTCAG TTATGATCGG TTTGGGTATG TAGGAACAAG 
    GATTCTCTTA AAACATACAG AGTCACAACC ATTAAGGAAT TGTATCACAA 
    AACTCGTCCC CGCCAGTCAG GCTCTTATTT ATCTGGGTCT CGTCGTCGAT 
    TCACTGTCCT AAATTTCCTG AGTTCCTGTA CCCCTAAACC ACCATCCCTA 
    GACGTTTTGT TAACCCTTTG GGTATTTGGT AGCAACTTTC ATTGTGGAGT 
    TGGATTGTGT GCTTAAGGTG AAAATATCAT TTGTGATAAA CCATGAGACT 
    TTGTTGTGCT GTCACCATGA CCACTGCTAT TAGCATCTGC AGATGCAACT 
    TTTGACAGTT ATACCAAAAG CTTCTCCCTG C

    Gene models with high sequence identity

    1. AT1G13950.1 [+] show detail - eukaryotic elongation factor 5A-1
      E-value: 5e-78; Score: 288.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Translation elongation factor, IF5A, hypusine site 0
    superfamily Translation protein SH3-like 3e-28
    superfamily Nucleic acid-binding, OB-fold-like 5.6e-28
    HMMTigr Translation elongation factor, IF5A 3.1e-56
    Gene3D Translation protein SH3-like, subgroup 5.2e-27
    Gene3D Nucleic acid-binding, OB-fold 3.6e-26
    HMMPanther INITIATION FACTOR 5A 5.3e-111
    HMMPanther Translation elongation factor, IF5A 5.3e-111
    HMMPIR Translation elongation factor, IF5A 1.3e-100
    HMMPfam Translation elongation factor, IF5A C-terminal 7.1e-31
    HMMPfam KOW 4.9e-05

    See the detailed result >

    AT1G13950.1

    Model type
    Protein coding
    Short Description
    eukaryotic elongation factor 5A-1
    Curator Summary
    Encodes eukaryotic translation initiation factor 5A (EIF-5A).In mammalian cells it functions as a shuttle protein that translocates mRNA from the nucleus to cytoplasmic ribosomes. Overexpression results in an increase in both primary and secondary xylem formation. In RNAi suppressed lines, xylem formation is reduced.
    Computational Description
    eukaryotic elongation factor 5A-1 (ELF5A-1); FUNCTIONS IN: ribosome binding, RNA binding, translation elongation factor activity, translation initiation factor activity; INVOLVED IN: translational initiation, xylem development; LOCATED IN: cellular_component unknown; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Translation elongation factor, IF5A, hypusine site (InterPro:IPR019769), Translation protein SH3-like, subgroup (InterPro:IPR014722), Translation elongation factor, IF5A (InterPro:IPR001884), Translation elongation factor, IF5A C-terminal (InterPro:IPR020189), Translation protein SH3-like (InterPro:IPR008991), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), KOW (InterPro:IPR005824); BEST Arabidopsis thaliana protein match is: eukaryotic elongation factor 5A-3 (TAIR:AT1G69410.1); Has 1356 Blast hits to 1355 proteins in 415 species: Archae - 264; Bacteria - 0; Metazoa - 366; Fungi - 246; Plants - 261; Viruses - 0; Other Eukaryotes - 219 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR