K14444

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14444

Determination of introduced cDNA(s)

J013106O15 - Rice Full-length cDNAs - Original: J013106O15

full reading [+] show sequence

GGCCCGTGGC ACAACTCTTG TGTTGACCCG CCTCGCGCTC GCCTGCCACC 
ACCGCCACCG CCAGCGAGCT GGATGAGCCA GCAGCCGCCC CCGCCCGCGG 
TTGCTTCCCC TCCCCACCAC GTCAAAACCC AACCCCAACC ATGATGCTCC 
TGCGCCACCA CCACCACCCC CACAGCGGCG CCGCCACCAC CAGCAGCAGC 
TGCAGCGGCG GCGGCGGCTG TTAGAGAGGA GGGCACACAC CACCACCGAC 
ACCGACACGC TCGCCATGCC ACCAAGCGAG GCGGCGGCGT GAGGCGACGC 
AGATCTGAAC GGAGGAGGAA TAGGAAGAAG GGAGGAGGAG GGGAGGGAGA 
GGAGTTGGAA GAGTTGGAGG AGGAGGAGAT CTCTTTCTTG TTCCCGCTCG 
ATGGAGCGGG GGGAGGGGAG GAGGGGAGAT TGCTCCGTGC AAGTGAGGAA 
GAAGAGAACG CGAAGGAAAA GCGATGGCCC TGATTCAATC GCTGAAACCA 
TCAAGTGGTG GAAGGAGCAA AACCAGAAGC TCCAGGAGGA GAATAGCTCC 
AGGAAAGCGC CATCCAAGGG GTCCAAGAAA GGGTGCATGG CTGGGAAAGG 
AGGTCCGGAA AATTCAAATT GTGCTTACCG CGGTGTCAGG CAACGGACAT 
GGGGTAAGTG GGTGGCTGAG ATCCGTGAAC CAAACCGTGG AAGGCGCCTA 
TGGCTAGGAT CATTTCCTAC TGCGCTGGAG GCTGCGCATG CATACGATGA 
GGCGGCAAGG GCAATGTATG GTCCCACAGC ACGTGTCAAT TTTGCAGATA 
ATTCCACAGA TGCCAACTCT GGCTGCACAT CAGCACCTTC ATTGATGATG 
TCTAATGGGC CGGCCACTAT ACCTTCTGAT GAGAAGGATG AGCTGGAATC 
TCCTCCTTTC ATCGTGGCTA ATGGGCCAGC TGTGTTGTAT CAGCCTGATA 
AGAAGGATGT GTTGGAACGT GTAGTCCCTG AGGTGCAGGA TGTTAAAACA 
GAAGGGAGCA ATGGCTTGAA ACGTGTTTGT CAGGAGCGGA AGAATATGGA 
GGTATGTGAA TCAGAAGGGA TCGTTTTACA CAAAGAAGTG AACATAAGTT 
ATGATTATTT CAATGTCCAT GAAGTTGTTG AGATGATAAT TGTTGAATTA 
AGTGCTGATC AGAAAACGGA AGTACATGAA GAGTACCAAG AGGGAGATGA 
TGGGTTTAGC CTTTTCTCCT ATTAGAGTAG TAGTCATGCT GCGGGTCAAT 
AGGAATATTT CATTCTAGCT GCTAGGGGAT ACTTCAAATA TCTGCAACCT 
GAAGCTTTGT AGTCATTTAC GGTTTTCGTC TTACTGGGTA ATAGCTTTAT 
ATATACTATA AGCCAACTGG TACAAGAAGT TGTACTGTGT GTTGAGTGCA 
CTGTGGTAAA AATGAATCTA TATTTAATG

Gene models with high sequence identity

AT5G05410.2 [+] show detail - DRE-binding protein 2A
E-value: 5e-37; Score: 152.00

InterPro Scan Digest

Program	Description	E-value
superfamily	DNA-binding, integrase-type	2.8e-22
HMMPfam	Pathogenesis-related transcriptional factor/ERF, DNA-binding	4.4e-16
FPrintScan	Pathogenesis-related transcriptional factor/ERF, DNA-binding	7.8e-09
FPrintScan	Pathogenesis-related transcriptional factor/ERF, DNA-binding	7.8e-09
ProfileScan	Pathogenesis-related transcriptional factor/ERF, DNA-binding	21.825
HMMSmart	Pathogenesis-related transcriptional factor/ERF, DNA-binding	4.1e-33
Gene3D	Pathogenesis-related transcriptional factor/ERF, DNA-binding	2.5e-21

See the detailed result >

AT5G05410.2

Model type: Protein coding
Short Description: DRE-binding protein 2A
Curator Summary: Encodes a transcription factor that specifically binds to DRE/CRT cis elements (responsive to drought and low-temperature stress). Belongs to the DREB subfamily A-2 of ERF/AP2 transcription factor family (DREB2A). There are eight members in this subfamily including DREB2B. The protein contains one AP2 domain. Overexpression of transcriptional activation domain of DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. Microarray and RNA gel blot analyses revealed that DREB2A regulates expression of many water stress
Computational Description: DRE-binding protein 2A (DREB2A); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: DRE/CRT-binding protein 2B (TAIR:AT3G11020.1); Has 5407 Blast hits to 5383 proteins in 247 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 5390; Viruses - 4; Other Eukaryotes - 11 (source: NCBI BLink).
Link: InterPro Scan - TAIR