K14638

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K14638

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14638

Determination of introduced cDNA(s)

  • J023134L09 - Rice Full-length cDNAs - Original: J023134L09

    full reading [+] show sequence

    Nucleotide sequence: J023134L09 - full reading 
    GAAAGGAACT TCTTTTCTGA AGACTGATTA AAAAAACATG ACCTTTGAAT 
    CAAGCTTGTG GCCTCTCAGA TAACGAACTT TTCCTGCCAC CTAGTTATGA 
    GTATAATATG GCTTACATTT ACACAGGTGA AAATGAAAAT GAGAAAAGAA 
    AAGGCACAAT AAGAAACAAC GGCTGATGAT TTATTATGTT CTGCCCTTCT 
    TTGTTGTGAC AAAGTATCTT GATCACAAGG ACGGAATTGG CAGGGTAGCT 
    GGCAGTTGCA GTAGCATTAA CTGCCAACTG TGTGCTGCTA ATCTTATTTG 
    ATCCAAATTA TATGGTTATT AATCAAATCC CATAGCCCCA TAATCCCCAA 
    CCATGCCCGA AGAAGCTGCT GGGATGGCGT CAGTCTTGCG GAGGCTCTAC 
    CTCTCTGTCT ACAACTGGAT TGTCTTCATC GGATGGGTTC AGGTGTCGTG 
    GTCCATGATA TTGGCATTGT TGGAGAAGAG ATATGAGGCC GTCTACGCTG 
    CTGTAGAGCA GCATCTGCTG TTCGCGCAGA CTGCTGCCAT CATGGAGATT 
    CTTCATTCCA TTGTAGGGTT GGTGAGGTCT CCAGTGTCTT CTACTCTTCC 
    ACAAATTACT GCAAGGTTGT TCATGATCTG GGGCATCTTG CGGAGCTTCC 
    CTGAGATACA CACTCATATT TTTGTTACCT CCTTGCTCAT AAGCTGGTGC 
    ATCACTGAGG TTACCAGATA TTCTTTCAAT GGCATGAAGG AGTCATTTGG 
    ATTTACACCT TCCTGGCTCT TATGGCTTAG GTACAGCACC TTCATAGTAT 
    GCTTTCCTAT TGGTATGGTT AGTGAGGTTG GCCTAGTCTA CATTGTTGTT 
    CCTTTCATGA AGGCATCTGA GAAATACTGC CTTAGGATGC CCAACAAATG 
    GAATTTCTCC ATCAAATACT TCTATGCCTC TGTCTTTTTC ATGGCTTTGT 
    ATGCCCCAGT ATACCCGCAC TTGTTCCACT ATTTGATCGT CCAGCGGAAG 
    AAGGCCTTGG CAAAATCAAA AACCACATAA CTACTCAAGA GCTATCTAAT 
    CTGGTTTCTG CTCGCAGCTA CAGCTCAGGT TGCTACCACT TCTTGAACAA 
    AGAAGTAAAA ATGTATTAGA ATGGAAATGC CGAACTACAC AGACTATCTT 
    TTCCCTTGGT AAATAAAAAT GGACATGAGT ACTATTTTCA TGATTTGTTA 
    ATGGTGTGTT GTACCAAACT TTGGCTTTTC CATATGTAAA TAAAATTGCA 
    CTCCATATCG ATGCC

    Gene models with high sequence identity

    1. AT5G10480.1 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
      E-value: 8e-72; Score: 268.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Protein-tyrosine phosphatase-like, PTPLA 3e-53
    HMMPanther PHOSPHATASE RELATED PROTEIN PTPLA 2.5e-59
    HMMPanther Protein-tyrosine phosphatase-like, PTPLA 2.5e-59

    See the detailed result >

    AT5G10480.1

    Model type
    Protein coding
    Short Description
    Protein-tyrosine phosphatase-like, PTPLA
    Curator Summary
    Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
    Computational Description
    PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR