K14743

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K14743

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14743

Determination of introduced cDNA(s)

  • J033075G20 - Rice Full-length cDNAs - Original: J033075G20

    full reading [+] show sequence

    Nucleotide sequence: J033075G20 - full reading 
    GACCCTTGTG CCCTTATCTC CTATGGTTGC TTCCACTGTC ACCAAAGGGG 
    GACTCGCCAT CTACCTTGTT CCTCAGTTCA TGCGAATTTC ATGCGGTCCT 
    GGGCTGTGAC TGGGAGTAGA TTGAGGGTTG ATCTCGTTTG CGGAGTAACA 
    AATTTGCAAG CCTACAACTG CTAGGGAGAA TTTGACTAAA AATGGGGTCG 
    TGTTGCAGCA GAGCTACGTC TCCAGATTCT GGTCGAGGAG GAGCAAACGG 
    TTATGGCTAT TCTCACCAGA CAAAACCTGC TCAGACAACT CCTAGCTATA 
    ACCATCCTCA GCCGCCGCCG CCAGCTGAGG TGAGGTACAC ACCATCAGCA 
    ATGAACCCTC CGGTAGTCCC ACCTGTGGTT GCCCCACCGA AGCCCACCCC 
    AGACACAATT CTTGGAAAGC CGTATGACGA TGTGCGCTCG GTTTACTCCC 
    TTGGAAAGGA ACTCGGAAGG GGACAGTTTG GGGTGACATA CCTGTGCACG 
    GAGATCGCTA GTGGTAAGCA GTATGCTTGC AAATCCATCT CTAAGCGCAA 
    GCTTGTGAGC AAGGCCGACA AGGAGGACAT TCGCCGGGAG ATTCAGATCA 
    TGCAGCACCT ATATGGACAA CAAAACATTG TCGAGTTCCG GGGAGCATAT 
    GAGGACAAGA GCAATGTCCA TGTTGTCATG GAGCTCTGCG CTGGTGGGGA 
    GCTGTTTGAT CGGATCATTG CCAAGGGGCA CTACTCAGAG CGTGCGGCTG 
    CTACCATCTG CAGGGCAGTG GTGAATGTTG TCAACATTTG CCATTTTATG 
    GGAGTGATGC ATCGTGATTT AAAACCGGAG AACTTCTTAC TTGCAACCAA 
    AGAAGAGAAT GCAATGCTCA AAGCCACTGA TTTTGGTCTC TCAGTTTTCA 
    TTGAAGAAGG AAAAATGTAT AGAGACATTG TTGGAAGTGC TTATTATGTT 
    GCTCCTGAAG TCCTTCGGCG GAATTATGGT AAAGAGATTG ATGTATGGAG 
    TGCAGGCGTT ATTTTGTACA TTCTTCTCAG TGGTGTTCCT CCATTTTGGG 
    CTGAAACTGA AAAGGGAATA TTTGATGCTA TTCTTCAAGG GGAGATTGAC 
    TTTGAGAGTC AACCATGGCC ATCAATATCC GAGAGTGCTA AAGACCTTGT 
    TAGAAAGATG TTGACACAAG ATCCAAAGAA AAGAATTACT TCGGCCCAAG 
    TTCTCCAACA TCCCTGGCTC AGAGATGGAG AAGCATCTGA CAAACCTATT 
    GACAGCGCTG TTCTTTCTAG GATGAAGCAA TTCAGAGCAA TGAACAAGCT 
    GAAAAAGATG GCTCTGAAGG TTATTGCTTC AAACCTTAAT GAGGAGGAGA 
    TCAAGGGCCT GAAGCAAATG TTCACAAACA TGGACACAGA CAACAGCGGG 
    ACCATCACAT ATGATGAACT CAAAGCAGGA TTAGCCAAAC TTGGATCAAA 
    GCTTTCAGAA GCTGAAGTAA AGCAGTTGAT GGAAGCTGCT GATGTAGACG 
    GCAATGGATC CATCGACTAT GTTGAATTTA TCACTGCCAC AATGCATAGA 
    CACAAGCTCG AAAGAGATGA GCATTTGTTT AAGGCATTCC AGTATTTTGA 
    CAAAGACAAC AGTGGCTTCA TCACAAGAGA TGAACTGGAA TCCGCTTTAA 
    TCGAGCATGA AATGGGCGAT ACAAGTACCA TAAAGGATAT TATATCAGAA 
    GTCGACACGA ATAATGATGG GAGGATTAAC TACGAGGAAT TTTGCGCTAT 
    GATGAGAGGA GGGGGGATGC AGCAGCCTAT GAGGCTCAAA TAGTCTGTTT 
    CCTTGTAGAA AATGATGTAC AATTACATTA GTAAACTGTT TTCGCCTTCT 
    TTTTTTTGTT CCATGGATGG TAGTCGACGG ATAAAAGGTT TACCCTGGAC 
    GCAGCTTTTG CATTGTTGAG ATGATTGTGT ATATTTTCTT GCCTTGCCCG 
    TGTATGGTGT AGTGATTTGA ATGTCCTCCC TTTTGTAAGT TGTTTCTGCA 
    AGTACACACA ATAAAAGAGC ATTTTTGCAC CTCAATTCTT CTGC

    Gene models with high sequence identity

    1. AT4G04720.1 [+] show detail - calcium-dependent protein kinase 21
      E-value: 0; Score: 735.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Protein kinase, catalytic domain 48.287
    ProfileScan EF-HAND 2 14.848
    ProfileScan EF-HAND 2 11.166
    ProfileScan EF-HAND 2 13.928
    ProfileScan EF-HAND 2 12.672
    Gene3D no description 1.3e-23
    Gene3D no description 5.3e-59
    Gene3D EF-hand-like domain 4.8e-20
    HMMSmart Serine/threonine-protein kinase domain 1e-97
    HMMSmart Tyrosine-protein kinase, catalytic domain 2.7e-12
    HMMSmart Calcium-binding EF-hand 1e-05
    HMMSmart Calcium-binding EF-hand 0.046
    HMMSmart Calcium-binding EF-hand 0.0044
    HMMSmart Calcium-binding EF-hand 8e-07
    HMMPanther Calcium-dependent protein kinase 1.5e-278
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 1.5e-278
    Coil coiled-coil 0
    HMMPfam Serine/threonine-protein kinase-like domain 7.5e-72
    HMMPfam EF-hand 1.5e-07
    HMMPfam EF-hand 2e-06
    HMMPfam EF-hand 9.7e-09
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    superfamily Protein kinase-like domain 4.7e-84
    superfamily EF-hand 1.1e-70

    See the detailed result >

    AT4G04720.1

    Model type
    Protein coding
    Short Description
    calcium-dependent protein kinase 21
    Curator Summary
    member of Calcium Dependent Protein Kinase
    Computational Description
    calcium-dependent protein kinase 21 (CPK21); FUNCTIONS IN: in 6 functions; INVOLVED IN: protein amino acid phosphorylation, N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: cultured cell; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 15 (TAIR:AT4G21940.1); Has 137194 Blast hits to 129287 proteins in 3977 species: Archae - 168; Bacteria - 14125; Metazoa - 50898; Fungi - 18038; Plants - 29874; Viruses - 499; Other Eukaryotes - 23592 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR