K14817

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K14817

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14817

Determination of introduced cDNA(s)

  • J033133F17 - Rice Full-length cDNAs - Original: J033133F17

    full reading [+] show sequence

    Nucleotide sequence: J033133F17 - full reading 
    GAGTACGCCC GGAGCTCGCA TCGCATCACC GGCTGAGAGA GATCCTAATT 
    CCGAGATGGC CACGACGCGG GGGCAGCTGC CCGTCGAGGA CGTGCTGCGC 
    GTGAACGGGA GCCGGCGATT CGCCGCCGCG CTGGCCGCCG CCTCCCCCTT 
    CGCCTCCCTC GCCGACGCCC TCCTCGCCGC CCGCCGCATC TGGCTCAACG 
    AGGTGGACGT CAACGGGTGG CTGGAGGCCT TCGCCGCGCA CCCGGCCATC 
    GGCACCACCT CCTCCTCCGC CCCCAAGTGG TGCAAGGAGG AGCAATCAGC 
    AGCGCTTGCA ACTGCCACTG ATTCGACCGC GCAGGAGTTG GCAGATTGGA 
    ATGCCAGGTA TAGGGAGAAG TTTGGGTTTG TGTTCATGAT ATGCGCGTCT 
    GGGAGGACTG CCCCTGAGGT CTTAGCGGAG CTTAAGAGGC GCTATGAAAA 
    TAGGCCAATT GTTGAACTTG AGATCGCAGC ACAGGAAGAA CTGAAGATAA 
    CTGAACTGCG TCTTGCAAAG CTTTTTGCAT CAGAGCCTGT TGCCCCCCCT 
    TCTTCAACGG TTGGAGGTCC TACTAGCCAA TCGGATAAAG CAGCAGACCG 
    CATGCGGATT ATTGGAGCAC ATCTTGGATC TCACACCCAG CATTCTGCCA 
    ATAAAGCTCC TGAAATTACA GGTAGCTCCA ACCGAACTCG CCCTCCGATC 
    ACAACCCATG TTTTGGATGT CGCTCGTGGA TCGCCAGCAT CAGGAATTGA 
    AGTTCACCTA GAGATGTGGA AGGATGCATC AACACCCCCA TCATTCAACA 
    ACAAAGATTT CAATGGATGG GCAACCCTGG GCTCATCGGT TACGAACAAC 
    GATGGCCGCA GCGGTCAACT GATGGACATT GTCAATAACG TCGCTCCTGG 
    TTTCTACCGC ATAAGTTTCA ACACCAGCAA GTATGCACCT TCAGGGTTCT 
    TCCCTTATGT GAGCATCATA TTTGAGATAA AAAAGAACCA GACGACCGAG 
    CATTTCCATG TCCCTCTGTT GCATTCTCCT TTTTCATTCA CCACTTACCG 
    TGGCAGTTAA GTTAGCCTGT CCCCAAGTCT GCGAGATGCT GCATATTCCA 
    TCTGAAAAGT TCGTGTGTAA TATTCCTCTT GGTAGCAAAA TGTCGGGAAA 
    ACAAGTTTGC TTTGGTAGCA AGGGTTGCCT GTTTATGTGC ACTGACATTA 
    TGATGCAAGA ATAAATTTGA TATACTAAAA TGTATACAGA AGCTTGATGT 
    CTATAGTACT AAAGTTTCAG TGCATGGTTT GGTACG

    Gene models with high sequence identity

    1. AT5G58220.1 [+] show detail - transthyretin-like protein
      E-value: 2e-82; Score: 303.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Transthyretin/hydroxyisourate hydrolase 0
    FPrintScan Transthyretin/hydroxyisourate hydrolase 2.3e-07
    FPrintScan Transthyretin/hydroxyisourate hydrolase 2.3e-07
    HMMPfam Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase 1.1e-34
    HMMPfam Transthyretin/hydroxyisourate hydrolase 1.7e-30
    HMMTigr Hydroxyisourate hydrolase 1.5e-29
    superfamily Transthyretin/hydroxyisourate hydrolase 5.8e-30
    HMMPanther URICASE AND TRANSTHYRETIN-RELATED 3.5e-20
    Gene3D Transthyretin/hydroxyisourate hydrolase 5.6e-24
    HMMPIR Bifunctional hydroxyisourate hydrolase/2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase 2.3e-300

    See the detailed result >

    AT5G58220.1

    Model type
    Protein coding
    Short Description
    transthyretin-like protein
    Curator Summary
    Encodes a transthyretin-like S-allantoin synthase protein that catalyzes two steps in the allantoin biosynthesis pathway by acting as a hydroxyisourate hydrolase and a 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase. Two alternatively spliced versions of the transcript give rise to a longer peroxisomally-targeted protein (AT5G58220.1 (called TTL1-)) and a slightly shorter cytoplasmic protein (AT5G58220.3 (called TTL2-)). Both have roughly equivalent enzymatic activity in vitro, but, allantoin biosynthesis is believed to occur in the peroxisome suggesting that the cytosolic form may participate in a different process.
    Computational Description
    transthyretin-like protein (TTL); FUNCTIONS IN: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity, hydroxyisourate hydrolase activity; INVOLVED IN: regulation of cell growth by extracellular stimulus, brassinosteroid mediated signaling pathway, protein homotetramerization, allantoin biosynthetic process; LOCATED IN: extrinsic to internal side of plasma membrane, peroxisome; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Bifunctional hydroxyisourate hydrolase/2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (InterPro:IPR017129), Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (InterPro:IPR018020), Hydroxyisourate hydrolase (InterPro:IPR014306), Transthyretin/hydroxyisourate hydrolase (InterPro:IPR000895); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR