K14912

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K14912

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K14912

Determination of introduced cDNA(s)

  • J023149L23 - Rice Full-length cDNAs - Original: J023149L23

    full reading [+] show sequence

    Nucleotide sequence: J023149L23 - full reading 
    GATCCTCCCC ACCTCCCTCC TCGCCGGCCG GAGTTCTCGT CTCCGGCGAC 
    CGCCGCCGCC GCCGCCCTCA CCGTCCGGTG GAGCTCCGGT GATCGACGCC 
    GCGATGTCGA GGTCTGGGGC CATGGATCTT GCATCTGGAC TCGGGGGGAA 
    GATCACCAAG GATGAGGTCA AGTCGGCTGT CGATGAGTAT GAGAAATACC 
    ATGGATACTA TGGAGGAAAA GAAGAAGCAA GAAAGTCCAA CTACACAGAC 
    ATGGTTAATA AATACTATGA TCTTGCCACC AGCTTCTATG AGTATGGTTG 
    GGGTGAATCC TTCCACTTTG CTCACAGATG GAATGGAGAA TCCCTACGTG 
    AAAGCATTAA GCGACATGAG CACTTTCTTG CCCTTCAGCT TGGAGTGAAA 
    CCAGGAATGA AGGTTTTGGA CGTCGGTTGT GGAATAGGCG GACCACTAAG 
    AGAAATTGCC AAATTTAGCT TGGCTTCAGT CACTGGATTG AACAACAATG 
    AGTACCAGAT AACTAGGGGA AAGGAGCTTA ATCGGGTAGC AGGAGTTAGT 
    GGAACTTGCG ACTTTGTGAA GGCAGACTTC ATGAAGATGC CGTTTTCTGA 
    TAACACTTTT GATGCTGTCT ATGCCCATTG AGGCAACATG CCACGCACCT 
    GATCCGGTTG GCTGCTATAA GGAGATCTAT CGTGTATTGA AGCCTGGCCA 
    GTGTTTTGCT GTGTATGAGT GGTGTATTAC TGATCACTAT GAACCGAACA 
    ATGCAACACA CAAGAGGATC AAGGATGAAA TCGAGCTTGG CAATGGCCTG 
    CCAGATATCC GAAGCACTCA ACAATGTCTT CAAGCTGCAA AAGATGCTGG 
    GTTTGAGGTT ATTTGGGATA AAGACCTTGC TGAAGATTCC CCGGTTCCTT 
    GGTACCTGCC CTTGGATCCA AGCCGGTTTT CCTTGAGTAG CTTCCGTCTG 
    ACTACTGTGG GGCGTGCAAT TACTCGCACA ATGGTGAAGG CATTGGAATA 
    CGTCGGCCTT GCCCCACAAG GAAGTGAAAG GGTATCTAAC TTTTTAGAGA 
    AGGCAGCTGA AGGTCTCGTC GAAGGTGGCA AGAAGGAAAT CTTCACGCCG 
    ATGTACTTCT TTTTGGTTCG CAAGCCTATC TCAGAATGAG CTCCCAGAAC 
    CACATTTCAG AGATAAGGCG AGGAGTTTGA AAGAAACATT ATGGCGGTCA 
    AAGAAACACC TGATGAAGCT CAGAAGCTTG AAGAGTTCTT ATGCGTACGA 
    AGAGATGCTG AAATGTGTTT AACTGGTGAA TTTTCGTCTG TTGGTTTGCC 
    GTGTAAAAAC CGTTTGATCA AGGCACTTGT CTGCAGGGTG CCTGTTTTAG 
    TCTAAGACGA TGAACTGACT GGTTTTTGTT TGCTCGTTTC TAAGTTGTTA 
    GTGACACATA AGTTAAGTTT GCAGTTCATT CGCCAGTGTG TATTGTAACT 
    TTGCAATAAG GCACCTGCTT GATAGCTTTC TTTTCATATT GTTCTTCGAC 
    AAATATAAGT GATGATTATT ACCTGT

    Gene models with high sequence identity

    1. AT5G13710.2 [+] show detail - sterol methyltransferase 1
      E-value: 2e-149; Score: 266.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 4.5e-30
    Gene3D no description 6.7e-06
    HMMPanther S-ADENOSYL-L-METHIONINE:DELTA24-STEROL-C-METH 3.2e-90
    HMMPanther METHYLTRANSFERASE 3.2e-90
    HMMPanther S-ADENOSYL-L-METHIONINE:DELTA24-STEROL-C-METH 9e-60
    HMMPanther METHYLTRANSFERASE 9e-60
    superfamily S-adenosyl-L-methionine-dependent methyltrans 1.8e-35
    superfamily S-adenosyl-L-methionine-dependent methyltrans 5.1e-16
    HMMPfam Methyltransferase type 11 1.1e-11
    HMMPfam Sterol methyltransferase C-terminal 3.5e-33

    See the detailed result >

    AT5G13710.2

    Model type
    Protein coding
    Short Description
    sterol methyltransferase 1
    Curator Summary
    SMT1 controls the level of cholesterol in plants
    Computational Description
    sterol methyltransferase 1 (SMT1); CONTAINS InterPro DOMAIN/s: Sterol methyltransferase C-terminal (InterPro:IPR013705), Methyltransferase type 11 (InterPro:IPR013216); BEST Arabidopsis thaliana protein match is: sterol methyltransferase 3 (TAIR:AT1G76090.1); Has 12253 Blast hits to 12250 proteins in 2286 species: Archae - 352; Bacteria - 8578; Metazoa - 91; Fungi - 457; Plants - 727; Viruses - 0; Other Eukaryotes - 2048 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR