K15025

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15025

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15025

Determination of introduced cDNA(s)

  • J023114D10 - Rice Full-length cDNAs - Original: J023114D10

    full reading [+] show sequence

    Nucleotide sequence: J023114D10 - full reading 
    GCCTTCTTCC GCGAGCGCCT CCACGGCCTT CGCCCTCGCC ACGGCCAGCC 
    TCTCCAGTAG CTTCCCCCCT TCCTCCGACT CCATCACCAG CCTCACCTTC 
    GCCTCCACCT CCTCCGCGCT CACCATCTCC TCGTCGTAGC CATCGATCAC 
    CACGCCCACC TTCATCTCCT CCACGATGAA CACCTTGTTC ATCCTCTGCT 
    CCGCGTACAG CGGCCAGCAC AGCAGCGGCA CCCCCGCCGT GATCCCCTCC 
    AGCGTCGAGT TCCACCCGCA GTGCGTGACG AACGCGGCCG TCGCCGCGTG 
    GCGTAGCACC TTGGCCTGCG GCGCCCATGA CTTGACCACC ATTCCTTTGT 
    CCGCCGTCCT CTCCAAGAAC CCCTCGGGGA GCAGCGCCGC CAGGTCTGGC 
    TCCGGCAGAT GCTCGAGCAG ATTGGCCGGA TCCTGACGCG GGCTCCGCAC 
    CACCCACAGG AACCGGTGGC CGGAGCTCTC GAGCCCGCGC GCTATCTCCT 
    TCAGCTGCGC CGCCGAGAAG GAGCCCATGC TGCCGAAGCA GAGGAACACC 
    ACGCTCCGCG CCGGCTGCGC GTCCAGCCAC GCGAGGCACG CGTGCCGCAC 
    CCCTCCTCCC TCCTCCTCGC CTGACGCCAC CAGCGGACCG ACGCAGTACA 
    CCGGCGGGGT GGGGCGGTCC GGGGAGCAAG CTCCCTCCCG GAGCGCACCC 
    ATGGCCCTCG CTTCAAGCCA CTCGTACGTG TTGAGCAGCA CGCCGCTCGC 
    CTCCATGGCG CGCGCGTACA GCTTGACCCG CGCCTTGTAA AACCGACCCT 
    CTCTGTCCTG CACGAGGGAC GGCATGTCCG ATGCCGGTAT CGGCGGGACG 
    CCGGGGAAAC GGAGGAGCGC CTTGCCCATG TCTCTGAGGT TGGTGGTAGT 
    CGTGGAGTAG TGGTGCATCA GGCCTAGGGA GGCGGCAAAG GCGCCCGCGG 
    CCGAGGTGAA GTATATGTAG GCGGGGACGT GGAGCGCCGC CGCGGCGTCG 
    ACTGCCTCGG CGCAGAATAT GTCGAGGACA AGCGCCGCGA CGGAGGGGAT 
    CGATCGGAGG AGCGACGCGA GGGAGGGCAC CGACCGGCGG AGCACGTCGA 
    GCATGAGCAA GAGGGGGTTC GGATCGGGGT CGGCGCAGGA CGGCGGCGGC 
    GGGAGGTGGT GGAAGGTGAT GGAGGGGTTG GAGGCGGCGA TGCCGGCGAT 
    GGTGGGGGAT CCCGTGGTGC CCGATCCGGG GGTGGCCGGG ACGGCGATGG 
    TGACGTCGAG GCCGCGGGTG AGGAAGAGCT TGGCGAGCTC GACCATGGGG 
    AGCAGGTGAC CCGCGCCCAT GGCGGCGTAT AGCACGACGT TGCGGCTGGG 
    CTTGGACTCC ATTAGCTCAG GACTCAGGAG CTCTCACTTT CATCGTCCTT 
    TGGAAGAGAG ATCTCAGTTG CGGTGTGGTC ACTGCTACGA AGGCGGGAAC 
    CATTTCCGTC TCCCGGTGAC GAAGGCGGGA AATTTGCTGG GTAAGGCTAT 
    ATTTACTCCG TGTGCGTCCA CTTCGGTGTG CGTGACCGCA CCATTTGCGT 
    CGCGGCCTCG CCTCGGCTTC TCCGTCCCGC CCGCGCAAGC AGCGCCCGCC 
    TCCTGTTCGA CGGAATGGCA CAGTGGGACG CGCTCGTGGA CGCCGCGCTT 
    GCAAGGCTGG CGTCGAGGAA CCTCCTGCGA GCCACGCGTC CCATCGCCCT 
    CGCGCCACCT CCAGCGCCGC CTGAAACCTT CGCCGGCCCT GGCCCATGGG 
    ACCGCGCCGC CGTCGAGATC CAGCTCGATC GCGCAACCCT CCACCAATGG 
    CTCGCCGAAG GTGGTGAAGC GACTGGCCAA GAAGAGGAGG TGGATGAGAA 
    ACTCATTCTA TTCTCCGGGA ATGATTACAT GGGCCTCAGC TCCCATCCGG 
    CAATCCGCAA GGCTGCAGTG AAGCTCAAGA GTACGGCATG GGACCTAGGG 
    GCTCTGCCTT GATATGTGGC TATACTACTT ATCACAAATT GGTGGAGGAA 
    TCTTTGGCAG AGTTGAAGAA AAAGGAGGAT TGCCTTCTTT GCCCAACAGG 
    ATTTTCTGCC AACATGGCTG TGATGACTGC TCTGGGAAGT GTGAGCTCTA 
    TTTTGTCTGT TGGGAGAAAA CCTGCAGAGG GTGAGAGAAT TGCAGTTTTC 
    TCGGATGCTC TCAACCATGC TTCTATCATT GATGGAATTC GCCTTGTTGA 
    GCGACAGCAA GAAGTAGTAG CATTTGTCTA CAAGCACTGT GACATGCATC 
    ATCTTGATTT CCTACTGTCC AGTTGCTCAA TTGAAAAGAA AGTTGTTGTC 
    ACAGATAGCC TGTTTAGCAT GGATGGTGAT TTTGCTCCAT TACCAGAACT 
    TGTCAAATTA CGCAGAAAGT ATGGGTTTTT GTTGGTCATC GACGATGCAC 
    ATGGAACACT TGTTTGTGGC GAGAATGGTG GTGGTGCACC CGAGCTGTTT 
    GGATGCGAAA ATGACATTGA CATAAGCGTT GGTACCCTAA GTAAGGCTGC 
    TGGCTGCCAG GGTGGTTTTA TAGTTTGCAG CAACCGATGG AAGAGGCTAA 
    TTCAATCGCG TGGCCGCTCA TTCATATTTT CAACTGCTTT GCCAGTTCCA 
    GTAGTTGCCT CTGTGTATGC TGCTCTCCAT GTCTCAAAGA AGGAGAGATG 
    GCGGAGATCA GTGATCTGGA GGCATGTGCA GTATTTTGCT TCTTTGACCA 
    AACTCGACTT AACTAGTCCC ATAATTTCCA TAGTAGTTGG AAGCGAAGAA 
    GCAGCACTTA GGGCTGGCAG GCATATGCTA AGATCTGGAT TTCATGTTAC 
    ACCAATTCGA CCACCCACAG TACCACAAAA CTCGTGCAGG TTGCGAATAA 
    CTCTAAGCGC TTCTCATTCC TTGGATGACA TCAAAAGGCT GGTAGATGCA 
    CTATCACCCT GGCTTCCTGA CAAACATGAT GAGCAGACAT ATGTTATGGC 
    GTCAAAACTT TAAGCAAGTC CATCGGAGAA TTCAGTTTCA ATATCTGATG 
    CCCTGCTAGT TGATATGAGG AAAAGGGGAT AAGTTGATTC TAGTTGACGC 
    GATTATTTGC ATATATCCAT TTATTTATGT TTGTATGATT GTATCACCAC 
    GATGTGAAAC ATAATTTATA TGATCTCCTG ATGCCCTACA AGTTGGGTAA 
    AGGAATTTAA AAAGTATTAT TTTATGACCT TTGCTATCTA TAAAGAATTA 
    AGTCGC

    Gene models with high sequence identity

    1. AT5G04620.2 [+] show detail - biotin F
      E-value: 2e-146; Score: 50.80

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Pyridoxal phosphate-dependent transferase, major domain 6.69996745106047e-65
    HMMPfam Aminotransferase, class I/II 1.2e-39
    HMMPanther PTHR13693 0
    HMMPanther PTHR13693:SF10 0
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 2.60000517657733e-64
    HMMPanther 8-AMINO-7-OXONONANOATE SYNTHASE 2.8e-12
    HMMPanther CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANO 2.8e-12
    HMMPanther UDP-GLUCOSYLTRANSFERASE 1.4e-114
    HMMPanther UDP-glucuronosyl/UDP-glucosyltransferase 1.4e-114
    Gene3D no description 7.3e-14
    HMMPfam UDP-glucuronosyl/UDP-glucosyltransferase 6.4e-29
    superfamily UDP-Glycosyltransferase/glycogen phosphorylas 1.4e-50
    PatternScan UDP-glucuronosyl/UDP-glucosyltransferase 0
    FPrintScan PRICHEXTENSN 8.5e-09
    FPrintScan PRICHEXTENSN 8.5e-09
    FPrintScan PRICHEXTENSN 8.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09
    FPrintScan PRICHEXTENSN 1.5e-09

    See the detailed result >

    AT5G04620.2

    Model type
    Protein coding
    Short Description
    biotin F
    Curator Summary
    The cDNA encoding 7-keto-8-aminopelargonic acid (KAPA) synthase, the first committed enzyme of the biotin synthesis pathway has been cloned and its molecular function confirmed (functional complementation of an E. coli mutant). The subcellular localization of the enzyme (cytosol) proves that the biotin biosynthesis in plants takes place in different compartments which differs from the biosynthetic route found in microorganisms.
    Computational Description
    biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR