K15101

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15101

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15101

Determination of introduced cDNA(s)

  • J013009O17 - Rice Full-length cDNAs - Original: J013009O17

    full reading [+] show sequence

    Nucleotide sequence: J013009O17 - full reading 
    GTCTCCATCC ATCGCCGCCG CCGCGCGCCG CCGCGTGAGC CGCCGGGGGG 
    CGGGCATTGG ATTGGACTTG GGGGCTAAAA GCAGCAGTTT CTTGTTTTTA 
    CTTCCTGGAG AAAGTGACGA TTTTGGTTAT TAAGCCAGTC GCTGGATTAC 
    GAGGAGGAGA GGGGATAGAT AGTTTGTGTC AGACGATGAT GATGCCGCTG 
    CGTTGCCTCT CCGAGCTGGC CCGCCGTCGC CGCGGCTGCT GCCGGCCTGA 
    TCACGCGGTT GATGCGATGA GAGCTGGGCT CCAATGTTTG ATGAGCACTT 
    GCTCCTCAAC CAAAGCTACT GATAATCATA GTTTCTCCAA CACATATGCA 
    ACTTCTTGTG CTAATTTGCC AGAGGTAGCA CCAAGCGAGG CGCCACTGTC 
    AGACATGCTC GTCGATTCAT TCGGGAGATT TCACAACTAC CTGAGGATCT 
    CCTTGACAGA GCGCTGTAAT CTGAGATGCC AGTACTGCAT GCCTGCAGAA 
    GGAGTTGAGC TCACGCCAAG CTCAGAGCTC CTGTCGCACG ACGAGATCAT 
    TCGAGTTGCC GATCTTTTTG TTACTTCTGG TGTGGATAAG ATCCGGTTGA 
    CTGGTGGAGA ACCAACCATT AGAAAGGATA TTGAAGATAT CTGCTTACAT 
    CTTTCTGGCT TGAAGGGACT GAAAACTCTT GCGATGACCA CGAATGGGCT 
    TGTTCTTTCT AAGAAGCTCC CAAGATTGAA AGAGTGTGGG CTCAATGCTC 
    TAAATATTAG TTTGGACACG TTGGTCCCTG CAAAGTTTGA GTTCATGACA 
    AGGCGTAAAG GGCACTCCAG AGTCATGGAA TCGATAGATG CTGCTATTGA 
    ACTTGGATTC CAATCTGTCA AGGTCAACTG TGTTGTCATG CGTGGAATGA 
    ATGATGATGA GATCTGTGAT TTTGTTGAAA TGACGAGAGA CAAGCCTGTC 
    AATGTCAGAT TTATTGAGTT TATGCCGTTT GATGGTAATG TTTGGAATGT 
    CAAGAAGTTG GTTCCTTATG CAGAGATAAT GGATAAAGTG CGCCAACGTT 
    TTAACGGTGT AGAAAGACTT CAAGATCATC CGTCAGAGAC GGCGAAGAAT 
    TTCAAGATTG ATGGTCATGC TGGAACAATT TCGTTTATTA CATCGATGAC 
    ACAGCATTTT TGTGCTGGTT GCAATAGATT ACGATTATTG GCTGATGGCA 
    ACTTGAAAGT GTGCTTGTTT GGCCCGTCTG AGGTGAGCTT GAGAGAGCCT 
    ATCCGAGCTG GTGTTGATGA TGCTGGACTC AGAGAAATAA TAAGCGCTGC 
    GGTTAAAAGG AAGAAAGCTA AACACGCTGG GATGTTTGAT ATTGCTAAGA 
    CAGCAAATAG ACCTATGATT CACATTGGTG GCTGAGATCA GTTTTACCAG 
    CTGAAAGTAC CTTGGCTCCT CCGTACGACG ACTGTGACGA ACTATCAACA 
    TGATAAAAAA ACAAAACAAA GATTTACATG ATCTTACCTT GATATGATGG 
    TAAAGTGAGG AACTCAAAAC TTCAAAGGGC AATACATACA GTTTGTTCAA 
    GATATAAAAA TGTTACTTGT GAAATAGTAG CAGTTGCAAA CCTCAGGCAG 
    TTGAAAGTTG AAGCATATCT GTACTTTTCG CTACTTGTAC GAGAAAGATG 
    TATCTGTTGT ATGATGTAAT GATGTAATGA TGTATGCTCC TCATAAATCA 
    TAATGCTAAT GTGTAGCTAA CTATTCTTTT GTGGCATCTT ACCGGTTAAT 
    AAAACGAAAT TATTGCATTT TTCT

    Gene models with high sequence identity

    1. AT2G31955.2 [+] show detail - cofactor of nitrate reductase and xanthine dehydrogenase 2
      E-value: 3e-163; Score: 572.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Elongator protein 3/MiaB/NifB 1.1e-08
    PatternScan MoaA/nifB/pqqE, iron-sulphur binding, conserved site 0
    HMMPfam Molybdenum cofactor synthesis C-terminal 1.6e-34
    HMMPfam Radical SAM 1.4e-27
    superfamily Radical SAM enzymes 4.9e-79
    HMMPanther MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A 1.3e-103
    Gene3D Aldolase-type TIM barrel 5.6e-12
    HMMTigr Molybdenum cofactor biosynthesis protein A 2.1e-139

    See the detailed result >

    AT2G31955.2

    Model type
    Protein coding
    Short Description
    cofactor of nitrate reductase and xanthine dehydrogenase 2
    Curator Summary
    COFACTOR OF NITRATE REDUCTASE AND XANTHINE DEHYDROGENASE 2. Encodes a protein involved in molybdenum cofactor biosynthesis. Homologous to E.coli moaA. Expression is abundant in all tissues examined, particularly in roots. Appears to have targeting signals for chloroplast or mitochondria.
    Computational Description
    cofactor of nitrate reductase and xanthine dehydrogenase 2 (CNX2); CONTAINS InterPro DOMAIN/s: Molybdenum cofactor synthesis C-terminal (InterPro:IPR010505), Aldolase-type TIM barrel (InterPro:IPR013785), Elongator protein 3/MiaB/NifB (InterPro:IPR006638), Molybdenum cofactor biosynthesis protein A (InterPro:IPR013483), Radical SAM (InterPro:IPR007197), MoaA/nifB/pqqE, iron-sulphur binding, conserved site (InterPro:IPR000385); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR