K15215

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15215

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15215

Determination of introduced cDNA(s)

  • J033094F19 - Rice Full-length cDNAs - Original: J033094F19

    full reading [+] show sequence

    Nucleotide sequence: J033094F19 - full reading 
    GGTCCGCAAG TCTCGTGCTC TCACTCTCCA CCTTCTTCTT CCCAGCTTCT 
    CTCGTCTCGG CTCAAGGCTC TAGCCATGGA CGCCGCCGCA ATGGAATCCC 
    TCATCCTGGA GCTCCACGCC ATCGAGGCCG TCAAGTTCGG CGCCTTCGTG 
    CTCAAGTCCG GCATCACCTC CCCGATCTAC CTCGACCTCC GCATGCTCGT 
    CGCCCACCCG CGCCTCCTCT CCACCGTCGC CTCCCTCCTC GGCTCCCTCC 
    CGGCGACCCG CCCCTACGAC CTCCTCTGCG GCGTCCCCTA CACCGCGCTC 
    CCCATCGCCT CCGTCCTCTC CGCCGCCGCC TCCGTCCCCA TGCTCCTCCG 
    CCGCTACCAC GTCACGCCGC ACGCCGCCGC CGAATGCCTC GACGGGTCCT 
    TCCGCGCCGG GGACGCCGTG CTCATCGTCG AGGACCTCGT CACCACGGGC 
    TCCTCCGTGC TCGAGACCGT CGCGCCACTC CGCGAGGTCG GGCTCGTCGT 
    CGCCGACGCC GTCGTGGTCA TCGACCGCGA GCAGGGCGGG AGGGAGAACC 
    TCGCCGCGAA CGGGGTCGCG CTCCACTCGC TCATGACGCT CACGGAGGTG 
    CTCGCCGTCC TCGTGAAGCG CGGGAAGCTG GGCGAGGAGA AGGCCCAGGA 
    GGTGAAGCGA TTCCTCGACG CAAACAGGAA GATGGCTGTG CCGGGACTGC 
    CGGTTAAACC GAAAGTAGTT AGGAAGGCAT TTTCTGAGAG AGCTGGATTG 
    GCGACGAATC CGATGGGGAG GAAGCTGTTC GAGTTGATGG AGGCCAAGCA 
    AAGCAACTTG TGTGTTGCTG CAGATGTCGG GACAGCAACA GAGCTACTTG 
    ATCTCGCCGA TAAGATTGGT CCAGAGATCT GCATGTTGAA AACTCATGTG 
    GATATCTTGT CTGATTTTAC ACCGGATTTT GGCCACAAGC TCCGTTCGAT 
    TGCTGAGAGG CACAGCTTTT TGATATTTGA AGACCGCAAG TTTGCAGACA 
    TTGGAAACAC AGTAACTATG CAATATGAAG GAGGAATATT CCGCATATTA 
    GACTGGGCTG ATATTGTTAA TGCACATATA GTTTCTGGAC CTGGAATTGT 
    AGAAGGCTTG AAGCTGAAGG GTCTGCCAAA AGGAAGAGGG TTACTTTTGC 
    TTTCTGAAAT GAGTTCAGCT GGCAACCTTG CTCATGGAGA TTACACTGCT 
    GCAGCCGTGA AGATTGCTGA GCAACATTCT GATATTGTAA TTGGATTTAT 
    ATCAGTAAAT CCTGCATCTT GGTCAGTGAC GCCGTCAAGT CCAGCGTTCA 
    TCCATGCTAC TCCTGGAGTT CAGTTGGTTG CTGGGGGAGA CTCGCTTGGT 
    CAACAGTACA ACACTCCTTA CTCTGTCATA AACGACAGAG GCAGCGACAT 
    AATTATAGTC GGTCGTGGGA TTATAAGGGC GAACAATCCT GCTGAGACCG 
    CGAGAGAGTA CCGCATCCAA GGATGGCATG CTTACCAATC CAGCTTATCA 
    TGAGAATGCT GTAAGGTCTC TCCCATTCTG TAACACGAAT GTAAGGGCAT 
    TCAGGCGATT GACTGACGCA ATAAAAGAGA GTATACGCAT GCTCTATCAT 
    CGCTGTGGTT CGTAATTCAC ATGCCCTATA TTGACACGCA CTATCCCAGC 
    AACAATTTCG TTTATGTGAT GGTAAATAAA TAAAGTTTGC TCCGAAC

    Gene models with high sequence identity

    1. AT3G54470.1 [+] show detail - uridine 5'-monophosphate synthase / UMP synthase (PYRE-F) (UMPS)
      E-value: 6e-165; Score: 577.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Orotidine 5'-phosphate decarboxylase, subfamily 1, core 6.1e-74
    superfamily Ribulose-phosphate binding barrel 3.5e-71
    superfamily PRTase-like 1.3e-36
    PatternScan Orotidine 5'-phosphate decarboxylase, active site 0
    Gene3D no description 7.1e-43
    Gene3D Aldolase-type TIM barrel 4.5e-98
    HMMPfam Orotidine 5'-phosphate decarboxylase, core 2e-75
    HMMPfam Phosphoribosyltransferase 3.8e-07

    See the detailed result >

    AT3G54470.1

    Model type
    Protein coding
    Short Description
    uridine 5'-monophosphate synthase / UMP synthase (PYRE-F) (UMPS)
    Curator Summary
    encodes the bi-functional orotate phosphoribosyltransferase/orotidine-5'-phosphate decarboxylase catalyzing the fifth and sixth step in the de novo pyrimidine ribonucleotide biosynthesis
    Computational Description
    uridine 5'-monophosphate synthase / UMP synthase (PYRE-F) (UMPS); FUNCTIONS IN: orotate phosphoribosyltransferase activity, orotidine-5'-phosphate decarboxylase activity; INVOLVED IN: response to cadmium ion, cellular response to phosphate starvation, pyrimidine ribonucleotide biosynthetic process, nucleoside metabolic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: guard cell, cultured cell; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), Orotidine 5'-phosphate decarboxylase, subfamily 1, core (InterPro:IPR014732), Orotidine 5'-phosphate decarboxylase, active site (InterPro:IPR018089), Ribulose-phosphate binding barrel (InterPro:IPR011060), Phosphoribosyltransferase (InterPro:IPR000836), Orotate phosphoribosyl transferase (InterPro:IPR004467), Orotidine 5'-phosphate decarboxylase, core (InterPro:IPR001754); Has 9174 Blast hits to 9124 proteins in 2964 species: Archae - 386; Bacteria - 5538; Metazoa - 208; Fungi - 955; Plants - 89; Viruses - 3; Other Eukaryotes - 1995 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR