K15421

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased size (PATO:0000587) (Seed::Shape::small) decreased length (PATO:0000574) (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15421

Determination of introduced cDNA(s)

J033020F16 - Rice Full-length cDNAs - Original: J033020F16

full reading [+] show sequence

GAGGCCGCCG CCTTCCTCGC CCGCTTCCCC GACGCCGAGG ACGACGCCCT 
CGACCTCCTC CTCGACGAGC TCGACAAGGA GCCGCTGCAG TCGTCGATTC 
TTGACCGGGA CGCGGTGCGG CGCGTGGTGG CGCTGCTCGT CGAGGCGGAC 
GAGGCGGTGG ATGCGGCGTC TCCGGCGGCC ACCAGCGCGC GCTCGGCGCT 
GCGGGTGGTG GATTCGTTCG TCGTGCCAAG GTTCCACTAC GATCCGATCA 
AGAAAGTCTT CTACGAGCAT ACCAGCAGAT TAGCCATACA TGGAGAAGCT 
GGAGATAAAG CAGCCCTGTA CAGGGACAGG TATCAGGTTC TGCTTCAGAG 
GCTAGCTCGT GATATATACT TCTCTAAGCC AGCTTTTGAC ACTGTTATGA 
CCGAAGATGA TAATTGTGAG ATAACTTCTA TTCAGTCCCT AATTGGGTGC 
ACTGGTCGGA GATGGATTAT GGGGGTCATA TCACAGTTGG AGGAACGTCA 
ATTCTACTTG GAGGATCTTA CTGGAGCTGT GCCAATTGAC TTATCGAATG 
CTAAAATCAC TTCAGGTTTC TTCGTTGAAA ATACTGTAAT TGTAGCAGAA 
GGGGAATTGC TTTCAAATGG CATTTTCCAG GTAAATACAT GTGGATTCCC 
TCCTTTAGAG GATAGAGAAG CATCACTTTC GATGCTTATG GGGCTAGATT 
TCTTCGGTGG AGGTGTCATA CCAACTGAAG AAACAGTAAG GCTATCAACC 
TTAGAGAAGA AGGCCATGAA TGATATGTTT GTTATACTTT CAGATGTTTG 
GCTTGACAAT TATGAGACCA TGGAGAAATT AGGTGTTGTT CTTGATGGTT 
ATGATAGTCT AGAAGCAGTT CCTTCCCTTT TTGTTTTAAT GGGCAATTTC 
TGCTCCCGGC CCTGCAATCT GGCATTCAAT TCATTTGAAG AACTCAGATT 
GCAGTTCGGA AAGCTTGGTG AGATGATTGC AGCTCGATCC AGGTTAAAGG 
AACACAGTCG CTTTTTGTTC ATTCCTGGTC CTGACGACGC AGGCCCTTCT 
AAAGCCCTTC CAAGGTGTGC ACTTCCGAAA TATCTAACTG AGGAACTGCA 
GAAGCACATC CCAAATGCAA TATTCGTAAG CAACCCTTGC AGGGTGAAGT 
TTTATACACA AGAAATTGTA TTTTTCCGGC AAGACCTCCT TTATAGGATG 
CGGCGGTCTT GTCTGATTCC CCCAACAACA GAAGAAACAA GTGACCCTTT 
CGAGCATCTG GTAGCAACCA TCACCCATCA GAGTCATCTT TGCCCATTGC 
CTCTTACAGT TCAGCCAATC ATATGGAGCT ACGATCATTG CCTTCGACTC 
TATCCTACTC CTCATACGAT AGTACTGGGC GACAAGAGCG ACCAGAAGGC 
CTTCAAATAC ACAGGAATAA CTTGCTTCAA CCCTGGTTCT TTTGCTAACG 
ATAGCACCTT TGCAGCTTAC CGTCCTTGCA CTAAGGAGGT TGAGCTTTCT 
GCATTAGAAA GCTAATATTA AAGAGATCAA AATGAGGCTC ACCAATTTGG 
CCAATTTGGT AGCCTGTACC ATCCAAACTA GCATTCTCCA AATCTTCGAT 
GAACTGCCTA TCGCCTTGGA CATCAGCCTG ATATTTATGA CTTGGACATA 
CAAGTTAAGG TGGAGGCCAT TTTCATGCAA GCTAATTCGA GGAGATGACA 
TAACATGCAT GACGCCATGG TTGGTGTTTC TCTCAAGCAA ACTGATGCTG 
ACATTTAGAT GGCATCATGG TAGTGGTTTT CTTATAACAA GGATTGAAAT 
TATACTTAGC ATAGCAGAAT ATTTGTGATG TATTTTGCTT AAGCCATTCT 
ACTTTGAAAT ATCTAGGCTT AAATTGTTGT AAACCAAGGT AACAAAAGTG 
CTGCTTTCTT GTTCCTTCAA AGGTTTAATC TCCC

Gene models with high sequence identity

AT5G22110.1 [+] show detail - DNA polymerase epsilon subunit B2
E-value: 0; Score: 634.00

InterPro Scan Digest

Program	Description	E-value
HMMPIR	DNA polymerase epsilon, subunit B	1.3e-208
HMMPfam	DNA polymerase alpha/epsilon, subunit B	3.8e-45
HMMPanther	DNA POLYMERASE EPSILON SUBUNIT B	3e-140

See the detailed result >

AT5G22110.1

Model type: Protein coding
Short Description: DNA polymerase epsilon subunit B2
Curator Summary: Encodes a protein with similarity to DNA polymerase epsilon subunit B an essential gene that is required for DNA replication. Homozygous mutants are embryo lethal. Expressed in meristematic , rapidly dividing regions.
Computational Description: DNA polymerase epsilon subunit B2 (DPB2); FUNCTIONS IN: DNA-directed DNA polymerase activity, DNA binding; INVOLVED IN: DNA replication, DNA-dependent DNA replication; LOCATED IN: epsilon DNA polymerase complex, nucleus; EXPRESSED IN: embryo, male gametophyte, apical meristem, female gametophyte; CONTAINS InterPro DOMAIN/s: DNA polymerase epsilon, subunit B (InterPro:IPR016266), DNA polymerase alpha/epsilon, subunit B (InterPro:IPR007185); Has 412 Blast hits to 394 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 125; Fungi - 144; Plants - 62; Viruses - 0; Other Eukaryotes - 81 (source: NCBI BLink).
Link: InterPro Scan - TAIR