K15446

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15446

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15446

Determination of introduced cDNA(s)

  • J023148I21 - Rice Full-length cDNAs - Original: J023148I21

    full reading [+] show sequence

    Nucleotide sequence: J023148I21 - full reading 
    GACTCTCTTC GCATGCAACA GCGGCCGCCA TTTTCCCTAG ATTCACCTTC 
    CATTGGTAGC TTGGTTGTTC ACCAATGGCG CACTTAGGGG CGGTCCTCTG 
    CATCCGTGTT GCTCTCGTCG TCGTCCTCCT CCCGTCGCTT CTGGCGACCG 
    TCGCCGTCGC GCACAACGAC ACCGGCGAGC ACAAGAACTA CCTCATCATC 
    GTGCGCAAGC CGTACGAGTA CGACCACAAT GTGTACAAGA CCGTGTCGAG 
    CTGGCACGCG TCGCTGCTGG CGTCCGTGTG CGACACGGCC AAGGAGGAGC 
    TCGCCACCGA CCCCGGCGCC GAGACGCGGC TCATCTACTC CTACCGCAAC 
    GTCGTCAATG GCTTCTGCGC CCGCGTCACC AGGGAGGAGG TGTACGAGAT 
    GGCCAAGAAG GACTGGTTCG TGAAGGCCAT CCCGGAGAAG ACGTACAAGC 
    TGATGACCAC GTACACGCCG AAAATGGTCG GGCTCACCGG CGCCCCGGCG 
    GCCTACCATG GCGGCTTGTG GAACAGGAGC AACATGGGCG AAGGGATGAT 
    CATCGGGGTG CTTGACAACG GCATCGCCGC CGGGCACCCG TCGTTCGACG 
    CGGCCGGGAT GGGGCCGCCG CCGGCCAGGT GGAAGGGCCG GTGCGACTTC 
    AACAGCTCAG TGTGCAACAA CAAGCTCATC GGCGCGCGGT CCTTCTTCGA 
    GTCGGCGAAG TGGAAGTGGA GAGGGGTCGA CGACCCGGTG CTCCCCGTCT 
    ATGAGCTGGC GCACGGCACG CACACCTCGA GCACGGCGGG CGGCAACTTC 
    GTGCCCGGGG CGAACGTGAT GGGCAACGGG TTCGGCACGG CGGCCGGCAT 
    GGCGCCACGC GCGCACCTCG CGCTCTACCA GGTGTGCTCC GAGGACAGAG 
    GCTGTGATCG GGATGACATA TTGGCGGCGA TGGATGACGC CGTGGACGAG 
    GGCGTCGACG TGCTCTCCAT CTCGCTCGGC GATGACGAGG CCGGAGATTT 
    CGCCGGGGAT CCCGTCGCGC TCGGGGCGTA CACAGCCATC ATGAGGGGCG 
    TCTTTGTCAG CTCGTCGGCC GGGAACAACG GCCCGAACCC ATTGACGGTC 
    TCCAACGAGG CGCCGTGGCT GCTCACCGTG GCGGCGTCCA CGACCGGCCG 
    GAAGTTCGTG GCCACCGTGA AGCTTGGCAC CGGGGTCGAG TTCGACGGCG 
    AGGCGCTCTA CCAGCCACCC AACTTCCCGA GCACGCAGTG GCCGCTGATA 
    GCGGACACCC GCGGTGACGG CACGTGTTCC GACGAGCACC TAATGAAGGA 
    GCACGTCGCC GGGAAGCTAG TCGTGTGCAA CCAGGGTGGC AACTTGACCG 
    GACTCCGGAA AGGGAGCTAT CTACACGATG CCGGCGCCGC CGGGATGGTT 
    CTCATCGGCC CTGAGTTCAT GGGCTCAATG GTCCAACCCA AGTCGCACAT 
    CCTCCCCGTG GCGCAAATCG TCTACTTGTC GGGGGAGGAG CTCAAGGCCT 
    ACATGAAATC CACGAAGAGT CCGACGGCGG CGCTGATCTA CAAAGGAACA 
    GTGTTCGGCG ACCGCAAGAC GCCGGAGGTG GCGCCCTTCT CGTCCCGGGG 
    GCCGAGCAGG CAGAACCAGG GGATCCTCAA GCCCGACATC ACCGGCCCCG 
    GGGTGAACAT CATCGCCGGC GTGCCCGTGA CCTCGGGCCT CGCCACGCCG 
    CCCAATCCAC TGGCGGCGAA GTTCGACATC ATGTCAGGCA CGTCCATGGC 
    GGCGCCGCAC CTCAGCGGGA TCGCCGCGCT GATCAAGAAA GCGCACCCGA 
    AGTGGTCACC GGCGGCGATC AAGTCGGCCA TGATGACAAC GGCCGACACG 
    TTGGACCGCC GGCGGAGGCC GATAACCGAC CAGAAAGGCA ACAATGCCAA 
    TATGTTCGGC CTCGGCGCCG GCTTCATCAA CCCGACGAAG GCCATGAACC 
    CGGGCCTCGT GTACGACCTG ACAGCGCAGG ACTACGTCCC TTTCCTTTGC 
    GGGCTCGGGT ACAGCGACCA CGAGGTGAGC TCCATCATCC ACCCGGCGCC 
    ATCGGTGTCC TGCAAGCAGC TGCCGGCCGT CGAGCAGAAG GACCTCAACT 
    ACCCGTCCAT CACCGTCTTC CTCGACCGGG AGCCCTACGT GGTGAGCGTC 
    AGCCGCGCCG TGACGAACGT CGGGCCCCGC GGCAAGGCGG TGTACGCGGC 
    GAAGGTGGAT ATGCCGGCGA CGGTGTTGGT GACGGTGACG CCGGACACGC 
    TCAGGTTCAA GAAGGTGAAC CAGGTGAGGA AGTTCACGGT CACCTTCAGG 
    GGCGCGAATG GCGGCCCGAT GAAGGGCGGC GTCGCCGAGG GGCAGCTCAG 
    GTGGGTCTCG CCGGACCACG TGGTGCGCAG CCCGATCGTC GTCTCCGCCC 
    AAAAGTTCTT GAACGGTAAC ACGAGCTCCT CCGATCACGC GGGCCATTGA 
    TTGTTGATTA CGTAGCTTTG CCAGTAAATG GGGTACTTGA TTAGACCCCC 
    AAGGATTATA AACTGCCCCC TGATGATTGT CCTCGAAAAA AGATGAAGAG 
    AAAGACAAAA TAACTCTATT AGAAGATGGA CCAAGATGCG AAGGGATTGC 
    GTGGCCGGAA AAAAAAAAAA GCAACATGGG ATTCTTTTTC CATGATGGCC 
    ATGGCCTGGA TATGTGCCCC TGAACCAAAC TGCTTCGATA TTGGGCTTGA 
    TGGGTTTGTT ACGGATTTAG ACCTTTATAC AATTAGCATA GCTAAAATTT 
    CGATCTTATT GTTGTGGGTT GGATATTGGA TGATAGCACT ACGGCACTAC 
    CTTTGTGGAT AAGGGATATA TCTCACACAC AATTCATTCA AATTTAGACA 
    TTTAGTAGTA GATAAAATGG TGAAAAAATT ACATTAACGG CACACAAATT 
    TTGATAAGAT TTTCTTTTGT TCTTTTGAAA GGTCTCCGCG GGGGTAGATC 
    TCCAATGAAC ACGCACATTC ACAAAATTTG ATAATAATTT TTTAGAAGTC 
    AACCAATACA ATATACTCGC ACAGAGTCAT TGATGCAAAT AGAAAAAAAA 
    AAACAAAAAC ACCAAGAAAC TGCTCGCCAA GGACGCCGTG CTGATAGAGA 
    AAATTTCCAC GCGTATGTAC TTAGGAAAGT TCCTTTTTAT TAAGAAAATA 
    GGATAAAAAC TTTGTCTCGT ATATATTAAT AAACCATAAG TATCC

    Gene models with high sequence identity

    1. AT3G14067.1 [+] show detail - Subtilase family protein
      E-value: 3e-145; Score: 513.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Peptidase S8/S53, subtilisin/kexin/sedolisin 9.3e-74
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.9e-15
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.9e-15
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.9e-15
    HMMPfam Peptidase S8/S53, subtilisin/kexin/sedolisin 3.9e-34
    HMMPfam Protease-associated PA 9.5e-18
    HMMPfam Proteinase inhibitor I9, subtilisin propeptide 4.7e-08
    Gene3D Peptidase S8/S53, subtilisin/kexin/sedolisin 6.4e-69
    HMMPanther SUBTILISIN-LIKE PROTEASE (PLANT) 2.6e-254
    HMMPanther Peptidase S8, subtilisin-related 2.6e-254

    See the detailed result >

    AT3G14067.1

    Model type
    Protein coding
    Short Description
    Subtilase family protein
    Computational Description
    Subtilase family protein; FUNCTIONS IN: identical protein binding, serine-type endopeptidase activity; INVOLVED IN: proteolysis, negative regulation of catalytic activity; LOCATED IN: apoplast, plasma membrane, vacuole, plant-type cell wall; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protease-associated PA (InterPro:IPR003137), Proteinase inhibitor, propeptide (InterPro:IPR009020), Peptidase S8/S53, subtilisin/kexin/sedolisin (InterPro:IPR000209), Peptidase S8, subtilisin-related (InterPro:IPR015500), Peptidase S8/S53, subtilisin, active site (InterPro:IPR022398), Proteinase inhibitor I9, subtilisin propeptide (InterPro:IPR010259); BEST Arabidopsis thaliana protein match is: Subtilase family protein (TAIR:AT5G67360.1); Has 8271 Blast hits to 7174 proteins in 1143 species: Archae - 230; Bacteria - 4646; Metazoa - 71; Fungi - 664; Plants - 1990; Viruses - 0; Other Eukaryotes - 670 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR