K15632

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15632

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15632

Determination of introduced cDNA(s)

  • J013035J20 - Rice Full-length cDNAs - Original: J013035J20

    full reading [+] show sequence

    Nucleotide sequence: J013035J20 - full reading 
    GATCAGGATC AAGCTCGTCT CGTTGATCAA TGGCATCAGA CAAGTGCTCA 
    TCGTTTCTAC TAGCAGCGGC AGCGGCTGCA GTGCTCGTCC TCGCGTCGGC 
    GCCGGTGGCT CATTCGTGGA GCAAGGAGGG TCACATGCTC ACATGCCGAA 
    TCGCGCAGGA TCTGCTGGAG CCAGCCGCGG CGCACGCGGT GAGGAACCTG 
    CTGACGGAGG AGGCGGACGG CGACCTGTCG GCGCTGTGCG TGTGGCCGGA 
    TCAGGTCAGG CACTGGTACA AGTACAGGTG GACGAGCCCT CTCCACTTCA 
    TCGACACCCC TGACAAAGCC TGCAGCTTCG TCTACTCAAG GGATTGCCAT 
    GGCGCGGATG GTGCGGAGGA TATGTGCGTC GCTGGCGCGA TCGCGAACTT 
    CACCTCTCAG CTTATGCACT ATAATCACGG CAGCGCCGAT CGCAAATATA 
    ACATGACTGA AGCACTGCTA TTCTTGTCAC ACTTCATGGG AGATGTTCAT 
    CAGCCAATGC ATGTTGGGTT CACGAGCGAC CAAGGGGGCA ATACGATTAA 
    TCTACGCTGG TTCAGGCACA AATCCAATCT CCATCATGTG TGGGATAGGG 
    AGATGATACT CACAGCTATT GCTGAATTCT ACGGCAAGGA CATGGATGCG 
    TTTCAAAAGG ACCTTGTGCA CAACTTCACC ACGGGAACGT GGTCTGATGA 
    CGTATCCTCT TGGGGAGACT GCGAGGATCT CCTGTCTTGC TCAACCAAGT 
    ACGCTACAGA GAGCATAAAT TTGGCTTGCA AATGGGCGTA CAACGATGTC 
    CGTGAAGGCG AAACTCTGTC AGATGACTAC TTCGGCTCAC GGCTCCCGAT 
    CGTGACACGA CGAATCGCGC AGGGGGGAGT TAGGCTGGCC ATGTTCTTGA 
    ATCGGCTCTT CGGGGAGCAC AACCGTGACG TCGCATCACC AGCCTGAACT 
    GATAGCTGCT TCAGAATTCA GAAAATTCAG AAGTTAACCA CACGTGTAAT 
    CTAACAGCCG CATCCATTTC ATTTTATTTT TACGTGATAC GTGACTCTAT 
    TTTTACGATG AATAGTTGAT GCCAACAATG CTGATTAATT AACCACATAG 
    TAATACAAGT AAGCAACGCT TGAAATCAAC AGGACATAGC AAACCAATTC 
    CATTCTTTTC TGATCATAAA GAGATGTACA TCCACATGTA TGATTAATTG 
    TATCTAGACA AGATTCAATG

    Gene models with high sequence identity

    1. AT1G11190.1 [+] show detail - bifunctional nuclease i
      E-value: 7e-120; Score: 427.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Phospholipase C/P1 nuclease, core 3.5e-90
    Gene3D Phospholipase C/P1 nuclease, core 5.7e-91
    HMMPfam S1/P1 nuclease 3.9e-83

    See the detailed result >

    AT1G11190.1

    Model type
    Protein coding
    Short Description
    bifunctional nuclease i
    Curator Summary
    Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
    Computational Description
    bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR