K15751

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15751

Determination of introduced cDNA(s)

J023070H24 - Rice Full-length cDNAs - Original: J023070H24

full reading [+] show sequence

GATGGATGGG CAGTGGGAGG AGCCGCAGCA CGCGGGGCGG CGGCGGCGGC 
AGCCGGAGCC CGGGAAGAAC CTCATCAAGA TCCCTTCCTA CCAGGAGGTG 
TTCGGGAGTG GCGCCTCATC CTCCGCCGCG CCGCCCTCCT ACAACCCTCC 
ACCCACCACC GCCGCCTCCT CCTCCTCCTC CTCGTCGTCG TTTTCGCAGG 
CCTTCTCCTT CCTCAAATCC ACCGAGTTCT ACTCCCCGCC ACCGCCGCCA 
CCACAGACGA CCACCCCAAG ACCGCCTCAG GCTAGCTCAG CTGCTCAGGC 
GCCAAAGAAC GCCATTCTTG TGAGCCATAG GCAGAAAGGG AATCCCTTAC 
TGAAGCACAT CAGGAACGCG AGGTGGACGT ACGCCGATAT AGTGCCGGAC 
TACGTGCTCG GGCAATCGTC ATGCGCACTG TACATAAGTC TCCGGTACCA 
TCTTCTACAC CCTGACTACT TGTATTATCG GATAAGAGAG TTGCAGAAGA 
ATTTCAAGCT CCGCGTCATT TTGTGCCATA TTGATGTTGA AGATGTCGTC 
AAACCTTTAC ATGAAGTTAC AAGAACATCA CTGCTACATG ACTGCACCCT 
CTTGTGTGGA TGGAGCCTGG AGGAGTGTGG TCGGTACTTG GAGACCATTA 
AAGTGTACGA AAACAAGTCA GCTGACAGCA TTCGTGAACA TATGGATAAT 
GACTATTTAT CAAGGTTGAC GCATGCTCTT ACATCCATTC GGCATGTTAA 
CAAGACAGAT GTTGTGACGC TTGGCTCATC TTTTGGGTCA CTTTCACAAG 
TTATGAATGC TTCTATGGAA GAACTAGCTC GATGCCCAGG AATTGGTGAG 
CGGAAGGTAA AACGACTTCA TGACACTTTC CACGAGCCAT TCAAACGGGT 
TTCCAGCCGC CCAAACATTG TAGTGCCTGA CACTCCTGAC AGAGAGAAGG 
CGTCAGGCCA ACCTTCATCG ACAAATGATG GTGCACAGGA TGCTGTGGAG 
AAGCCAGACG CATCCGTGAA CAAGAAGAGT TCTAATGTAA GATCAGCCCT 
TACTGAAGCC TTTGCCAAGT ATTCAGAAAA GATACGCAAC CAGAATCGTG 
ATTCAACAAA TGTGGCTGGT GAAGGTACTA GCAAGTCAAC TATGGGAGAT 
GAAAAAGACC AATAATTAGA TTAGGGGTAG CTTGGTAGGA GGTCCATGAC 
AGTTGAGAAC AAAAGGAGTA AGAAGAAACT AGCTGAGATT CAATTGACCT 
ATAAAGCTAC CAGTTCAACT AAAGGAAGTA GCTTACGGAC TTAAGGCTGC 
TAGGGACTCT GGGGCCAGAA GATGCTTTTC CCGCATTCCT GTTTATGCAG 
ATCAGACGAG AAGGGCTATC TCTTTACTAG AATCCGATAT GATAGAAGGA 
GCAGCTCTAG CTTAAGCCTA AGTTGATCCT TACTGTTCAA GATGTCTACA 
ACACAGTAAG GTAGGCGAAG CTACTAGTTT ACTTGCTTGA AAAAGGCAAC 
CTTAGCTGAT TCACATTACC TCTTGTTATT TCAATCCTTC CATGTCCCCC 
ATCAAAGCAG CTACAATGAG TAAGGAAGTT AGCAGATGAA AAATACCAAT 
AATTAGATTA GCGGTAGCTT GGTAGGAGGT CCATGACAGT CCAAAATGCT 
GAGTTCAAAT GTAATGTCCC CGGTTTGGTA ATGGTTGTTG TAGCTGCAGC 
TTTTTCTGTA TTTTTCCGTG TAATTTGCTC CAAAGAGAGA CAGGAACACC 
GCCAATTTTA TTAGCAAGGA TGAGTTTC

Gene models with high sequence identity

AT3G05210.1 [+] show detail - nucleotide repair protein, putative
E-value: 4e-102; Score: 369.00

InterPro Scan Digest

Program	Description	E-value
HMMSmart	Helix-hairpin-helix DNA-binding motif, class 1	1
superfamily	RuvA domain 2-like	1.9e-18
HMMTigr	DNA repair protein rad10	2.80000504261099e-49
Gene3D	G3DSA:1.10.150.20	3.39999972437719e-05
BlastProDom	DNA repair protein rad10	0
HMMPanther	PTHR12749	1.30000495407324e-92
HMMPfam	DNA repair protein rad10	1.9e-26

See the detailed result >

AT3G05210.1

Model type: Protein coding
Short Description: nucleotide repair protein, putative
Curator Summary: encodes a homolog of human ERCC1 protein (yeast RAD10), which is a DNA repair endonuclease. Mutants are sensitive to UV-B and gamma radiation (G2 cell cycle phase arrest) and are defective in dark-repair of pyrimidine pyrimidone dimers. This protein incises the 5' end of damaged DNA, similar to ERCC1/RAD10.
Computational Description: ERCC1; FUNCTIONS IN: 5'-flap endonuclease activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Helix-hairpin-helix DNA-binding motif, class 1 (InterPro:IPR003583), DNA repair protein rad10 (InterPro:IPR004579), RuvA domain 2-like (InterPro:IPR010994); Has 658 Blast hits to 452 proteins in 188 species: Archae - 0; Bacteria - 4; Metazoa - 166; Fungi - 153; Plants - 44; Viruses - 0; Other Eukaryotes - 291 (source: NCBI BLink).
Link: InterPro Scan - TAIR