K16231

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased width (PATO:0000599) (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K16231

Determination of introduced cDNA(s)

J013134O04 - Rice Full-length cDNAs - Original: J013134O04

full reading [+] show sequence

GGTGATGCTC TCGCACATCG ATTGGTGGAA CAGAGTATCC CGGTCAAAGC 
CATTAGCCAC CATCCCGACG TCTTCCTCCA TCTCGCATAT CTCTGCAATG 
GCAGGGAATG CGTCAGTGAT GCGGAGGCTC TACCTCTCTT TCTACAACTG 
GATTGTCTTC ATCGGATGGG TTCAGGTGTC GTGGTCCATG ATATTGGCAT 
TGTTGGAGAA CGGATATGAG GCCGTCTATG CTGCCGTAGA GCAGCATCTG 
CTGTTCGCGC AGACTGCTGC CATCATGGAG ATTCTTCACT CCATTGTAGG 
TGAGATATCC TCTTTCTTTA TAAAGAAAAT AATAAGTAAT AAGGGTTGGT 
GAGGTCTCCA GTGTCTTCTA CTATTCCACA AATCACTGGA AGGTTGTTCA 
TGATCTGGGG CATCTTGTGG AGCTTCCCTG AGGTAATATC AGGACCTTAC 
TCCAAGTTAT AAGAAGGTTT ATATCTTAAA AAACAAGTTT TGGATTAGAA 
AGCACTTCTT AGAATGATGA AACATAGATG TTAGGTAATG TTGCAGCTAT 
TAGAACATAT CCCATACTTT CATTTATGAT CAAAATGACT CTAAGTAGGC 
ATTGGACAAA CCGAAGCTAC AGAAATTGCT TAAGTAATGA AGCGGATGCA 
ATTACATCAT GGAGAAGGAC AAAATAGTGT TCTTTTCCTA GATTCCTATT 
TCATGTATTT AACTTCTAAT CAAGTAATTG TTTTATTTAT AAACAATATC 
GGTGAATCAC TGTCCACATG TTTTGCAGAC ACACACTCAT ATTTTTGTTA 
CCTCCTTGCT CATAAGCTGG TGCATCACTG AGGTATATGT TACTTCCTAA 
AACTTCTCAC CAAATTACTC ACTTGGAAGA TCATGGATTT CGGATGCAAT 
TTTATTATAG TTCTGCTTCT TGAAATGACA GTAGTTGTTG AAGTTCCTGA 
AAGAAAATCC CAAGCTTTGA AAGTTCCTGT GTTGAGCTTA GTTGAACAAA 
TATTATGAAA TTTTCTTCAC TTCAGAGCAT GACTTTGTAG ACAAAAAATT 
ACTAATTCCT TGCAGGTTAC CAGATATTGT TTCTATGGCA TGAAGGAGTC 
ATTTGGATTT ACACCTTCCT GGCTCTTATG GCTTAGGTTG GCTCTAGTCG 
TAAACAAATC ATAATATGTA GCATAAACTT CAGCGAATTC TCTTAAAAAT 
ATGCTGCTGT TACTTCCATA CTAATTACTT TGCAGGTACA GCACCTTCAT 
AGTATGCCTT CCTGTTGGTA CGGTTAGTGA GGTTGGCCTA ATCTACATTG 
TTCTTCCTTT CATGAAGGCA TCTGGGAAAT ACTGCCTTAG GATGCCCAAC 
AAATGGAATT TCTCCTTCAA CTACTTCTAT GCCTCTATCT TTTTCATGGT 
TTTGTATGCC CCAGTATATC CACGCTTGTT TCGCTACTTG ATCGCCCAGC 
GGAAGAAGGC CTTGGCAAAA GCAAAAACCA CATAACTATA CTCAAGAGCT 
ATCTACTCTG ATTTCTGCTC GCGGCTACAG CTCAGGTTGC TACCGTTTCT 
TGAGCAAATA AGTAAAAGTG TATTAGAATG GAAATGCCGA AATACAGACT 
ATCTTTTCCC TTGGTAAATA AAAATGTACA CGAGTACCAT TTTCATGATT 
TGCTAATGGT GTGTCCTACC AAAGATCGGC TTTTCCTTCT GTAAATAAAA 
TTGTACTCCA TATCTGTGCC C

Gene models with high sequence identity

AT5G10480.3 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
E-value: 5e-27; Score: 54.70

InterPro Scan Digest

Program	Description	E-value
HMMPanther	PHOSPHATASE RELATED PROTEIN PTPLA	1.1e-12
HMMPanther	Protein-tyrosine phosphatase-like, PTPLA	1.1e-12
HMMPanther	Protein-tyrosine phosphatase-like, PTPLA	2.8e-21
HMMPfam	Protein-tyrosine phosphatase-like, PTPLA	3.9e-21

See the detailed result >

AT5G10480.3

Model type: Protein coding
Short Description: Protein-tyrosine phosphatase-like, PTPLA
Curator Summary: Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
Computational Description: PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1).
Link: InterPro Scan - TAIR