K16546

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K16546

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
T2  
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)

Invisible phenotypes

Original: K16546

Determination of introduced cDNA(s)

  • J023117K20 - Rice Full-length cDNAs - Original: J023117K20

    full reading [+] show sequence

    Nucleotide sequence: J023117K20 - full reading 
    GGTCTTCCTC TGAACCCTGA ACCAATCCCG AACTCTGAAC TCTGAAGGCT 
    GCAGAGACAG GCAGAGAGAT ATGAGGGAGA TAATAAGCAT CCACATAGGG 
    CAGGCGGGCA TCCAGGTGGG GAATTCTTGC TGGGAACTAT ACTGCCTCGA 
    GCATGGCATC CAGCCGGACG GCCTCATGCC CAGCGACACA ACTCCTGGAA 
    TTGCAAGGGA TGCATTCAAC ACGTTCTTCA GCGAGACGAG TTCAGGCAAG 
    CACGTCCCAA GGGCGTTGTT CGTCGACCTG GAGCCCACGG TGATTGATGA 
    GGTGAAGACC GGGCCATACC GCCAGCTCTT CCACCCAGAG CAGCTCATCT 
    CCTACAAGGA AGACGCTGCT AACAACTTTG CTCGTGGGCA CTACACAGTT 
    GGAAGAGAGG TGGTAGATCT TTGCCTTGAT CGACTAAGAA AACTGGCAGA 
    TAACTGCACT GGTCTCCAAG GTTTTCTGGT TTTCAATGCT GTTGGTGGAG 
    GAACCGGCTC TGGACTTGGT TCATTACTTT TGGAGCGCCT ATCAGTAGAT 
    TACGGTAGGA AGTCGAAGCT CGGGTTCACA ATATATCCTT CACCACAGAT 
    CTCAACGGCC GTGGTGGAGC CTTACAACAG CGTGCTCTCG ACCCACTCCC 
    TGATCGAGCA CACCGACGTC GTCGTCCTCC TGGACAACGA GGCCATCTAC 
    GACATCTGCA AGAGGTCGCT GGACATCGAG CGGCCGACGT ACACCAACCT 
    GAACAGGCTC ATCTCCCAGG TGATCTCGTC GCTGACCACG TCGCTGCGGT 
    TCGACGGCGC CATCAACGTC GACATCACCG AGTTCCAGAC CAACCTGGTG 
    CCGTACCCGA GGATCCACTT CATGCTGTCC TCCTACGCCC CCATCATCTC 
    CGTCGAGAAG GCGTTCCACG AGCAGCACTC CGTCCCGGAG ATCACCAACT 
    CCGTCTTCGA GCCCGCCAGC GTCATGGCCA AGTGCGACCC CAGGCACGGC 
    AAGTACATGG CCTGCTGCCT GATGTACCGC GGCGACGTCG TCCCCAAGGA 
    CGTCAACGCC GCCGTCCACT CCATCAAGAC CAGGAGGACT GTGCAGTTCG 
    TCGACTGGTG TCCGACAGGG TTCAAGTGCG GGATAAACTA CCAGCCGCCG 
    ACGGCGGTGC CGGGAGGGGA CCTGGCGAAG GTGAGGAGGG CGGTGTGCAT 
    GATCAGCAAC AACACGGCGG TGGCGGAGGT GTTCTCGCGG ATCGACCGCA 
    AGTTCGACCT CATGTACGCC AAGCGCGCGT TCGTGCACTG GTACGTGGGC 
    GAAGGGATGG AGGAAGGGGA GTTCTCCGAG GCCAGGGAGG ACCTCGCCGC 
    GCTGGAGAAG GACTACGAGG AGGTCGGCGC CGAAGTCGAG GACGACGACG 
    AGGAACAGGG CGAGTACTGA TCGGGCTAGC CTGCTAGGTG AATCTAGCAC 
    GGTAGATAAA CCGCAAGTTT GTAAGGTCGG TGGAAGGGGG TCTCTCGGTT 
    TACATGGACT CTTGTGACTT TGTGGTGTGA GAGTGGGTTT TGTTGTACTT 
    GCATCACAAA AAATAAAAGA ATTCGTCAGA AATC

    Gene models with high sequence identity

    1. AT5G19780.1 [+] show detail - tubulin alpha-5
      E-value: 0; Score: 786.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Tubulin/FtsZ, GTPase domain 2.2e-118
    Gene3D Tubulin/FtsZ, 2-layer sandwich domain 4.6e-48
    Gene3D no description 2.8e-21
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Alpha tubulin 4.2e-109
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    FPrintScan Tubulin 4e-88
    HMMSmart no description 5.5e-69
    HMMSmart Tubulin/FtsZ, 2-layer sandwich domain 1.4e-49
    superfamily Tubulin/FtsZ, N-terminal 7.3e-102
    superfamily Tubulin/FtsZ, C-terminal 9.4e-82
    HMMPanther Alpha tubulin 9.2e-230
    HMMPanther Tubulin 9.2e-230
    HMMPfam Tubulin/FtsZ, GTPase domain 2.4e-70
    HMMPfam Tubulin/FtsZ, 2-layer sandwich domain 4.1e-50
    Coil coiled-coil 0
    PatternScan Tubulin, conserved site 0

    See the detailed result >

    AT5G19780.1

    Model type
    Protein coding
    Short Description
    tubulin alpha-5
    Curator Summary
    Encodes an isoform of alpha tubulin. Closely related to adjacent gene TUA3 suggesting recent duplication.
    Computational Description
    tubulin alpha-5 (TUA5); FUNCTIONS IN: structural constituent of cytoskeleton; INVOLVED IN: response to cadmium ion, microtubule-based process; LOCATED IN: tubulin complex, cytosol, cell wall, membrane; EXPRESSED IN: 6 plant structures; CONTAINS InterPro DOMAIN/s: Alpha tubulin (InterPro:IPR002452), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Beta tubulin, autoregulation binding site (InterPro:IPR013838), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin alpha-3 (TAIR:AT5G19770.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR