K16716

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K16716

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K16716

Determination of introduced cDNA(s)

  • J023101D03 - Rice Full-length cDNAs - Original: J023101D03

    full reading [+] show sequence

    Nucleotide sequence: J023101D03 - full reading
    GCCACTCGAA GAGCTCGGCG GTGGAACAGC TCGCTCGATC ATGGCCGCCG 
    CCGCCGCCGC CTCAAGGGCG CTCTGGGCTT GCCGCACCGC CTCCTACCTT
    CGGATCTCCT CCTTCCCCAG GGCTTTCTCC ACCGTCCTTA AAGATCTAAA
    ATATGCTGAT ACTCATGAGT GGGTGAAGGT TGAGGGTGAT TCAGCAACCA
    TAGGGGTCAC AGACCATGCT CAGGACCATT TGGGTGATGT TGTGTATGTT
    GAGCTTCCAG AAGTTGGTTC CACAGTATCA CAAGGAACGA ACTTTGGTGC
    GGTTGAAAGT GTGAAAGCAA CCAGTGATAT CAATGCACCA GTATCTGGAG
    AGATTATTCA AGTAAATGAT GAACTAAGTG AAAAACCAGG ATTTATAAAT
    GGAAGTCCAT ATGAGAAGGG ATGGATCATC AAGGTTAAGA TAAGTGATCC
    AAGTGAGTTA AACTCGCTGA TGGATGATGA GAAGTACAAG AAGTTTTGTG
    AGGAAGAAGA TGGCAAACAC TGATCAATCC CCTAACTGCT GGATTTCAAG
    AAACTATGCA GCAACTCACC ATCATGACAA TGAGCCATCT CGCCTCTCCT
    ACCACATTAT ATTATTCTGG ATAGAGGAGT CCCAAGGAAT GTAATAAGCT
    GAAACATTTT TCTTGCAAAT CAGACATGAA AATTTTGTAG TGTCCTGATC
    ATTCCGTTTG TGCTCCATTT GAAATTCAGC TCTTGATTTG CTTATCATCT
    GAATACAGGT GGAGGATTGG GTTTGTTTAA ACTTTGATTA AGTGACAGTG
    ACTTGATTGT ATTGAGATTG C

    Gene models with high sequence identity

    1. AT2G35120.1 [+] show detail - Single hybrid motif superfamily protein
      E-value: 1e-57; Score: 219.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Glycine cleavage H-protein 1.1e-49
    HMMPanther Glycine cleavage H-protein 2.4e-66
    superfamily Single hybrid motif 3.2e-48
    HAMAP Glycine cleavage H-protein 29.41
    Gene3D no description 2.9e-48
    HMMTigr Glycine cleavage H-protein, subgroup 6.1e-68
    PatternScan 2-oxo acid dehydrogenase, lipoyl-binding site 0

    See the detailed result >

    AT2G35120.1

    Model type
    Protein coding
    Short Description
    Single hybrid motif superfamily protein
    Computational Description
    Single hybrid motif superfamily protein; FUNCTIONS IN: glycine dehydrogenase (decarboxylating) activity, ATP binding; INVOLVED IN: glycine catabolic process, glycine decarboxylation via glycine cleavage system; LOCATED IN: mitochondrion, glycine cleavage complex; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 2-oxo acid dehydrogenase, lipoyl-binding site (InterPro:IPR003016), Single hybrid motif (InterPro:IPR011053), Glycine cleavage H-protein (InterPro:IPR002930), Glycine cleavage H-protein, subgroup (InterPro:IPR017453); BEST Arabidopsis thaliana protein match is: Single hybrid motif superfamily protein (TAIR:AT1G32470.1); Has 7147 Blast hits to 7147 proteins in 2201 species: Archae - 168; Bacteria - 4552; Metazoa - 187; Fungi - 130; Plants - 205; Viruses - 0; Other Eukaryotes - 1905 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR
  • J013170E20 - Rice Full-length cDNAs - Original: J013170E20

    full reading [+] show sequence

    Nucleotide sequence: J013170E20 - full reading
    GGTACTACGC CAATGGCTCT CCGCTGCCTA GCTGCTGCCG CAGTACAGCT 
    TGATCGTTGC CCGTAACCTC CCGTGGCTCC GATCCCGTCT CCGGCGCGGC
    GCGCGGCTCG GCGTTGAGAC AGTTTGCCGC CTCATGTCCC GGGAGCTGGA
    GCTGCTGCTG TTTTTGCTGG CCCTACGGGC GAGCGGCGAG GTCGTCGCGG
    GTTCGGCGGA GGCAGCGGCA GCGGCAGCGG CGTGGCCCGC GCTGCAGGTG
    GGGTTCTACC ATGCCAAGTG CCCTGTCGCG GAGGACGTCG TGCTGGGCGA
    GATGAGGATG ATCCTCGAGG AGGATCCCAC GCTCGCGCCG TCTCTGCTCC
    GGATGCACTA CCACGACTGC TTCGTCCAGG GGTGTGACGG ATCGATCATG
    CTGAGGTCGA GGAGCGGGAA GGGGGAGAGG GACGCGACGC CCAACCGGAG
    CATGCGGGGC TACGACGCCA TCAATCGGAT CAAGGCGAGG CTGGAGACCG
    TCTGCCCGCT CACCGTCTCC TGCGCCGACA TCATCGCCAT GGCGGCGAGA
    GACGCCGTCT ACCTGAGCAA GGGGCCGTGG TACGACGTGG AGACCGGGAG
    GAGGGACGGC GACGTGAGCG TGGCCGAGTA CGCCGAGAAC GACCTGGCGC
    CGCCGGACTC CAACATCGTC GACGTCAAGA CCTTCTTCAG CGTCAAGTCT
    CTCAACGCCA AGGACATCGC CGTGCTCTTC GGATGCCACA GCATCGGGAC
    GTCGCACTGC GGGGCGTTCC AGAAGCGGCT GTACAACTTC ACCGGAAGGA
    TGGACCAGGA CCCGTCGCTG GACGCCGGCT ACGCGGCGAA GCTCAAGAAG
    CTCTGCCCGC CGGGGCACGG CCACGACCAC GACCACGACG GCCATGGCGG
    CGCCGGCGGC GCGGCCAAGG TGCCCATGGA CCCCGGCAGC GGCTTCACAT
    TCGACCTGAG CTACTACCGG CACGTGCTCG CCACGGGCGG CCTGTTCCAG
    TCGGACGGCA GCCTCCGCGA CGACCCCGTC ACGAGGGGCT ACGTCGAGAA
    GCTCGCCAAC GCGTCGTCGT CGGAGGAGTA CTTCGCCGAC TTCGCGGCGG
    CGATGGTCAA GATGGGCCGC ACCGACGTGC TCACCGGCGA TCTCGGGGCG
    GTCAGGCCGA CGTGTGACAG CCTCGTCGAC TAGTTTATTC GGCGTCCCGC
    CACATTTGTG ATCTGTAGAA TTTTATATGG TTTTCTCTGT AACATATATG
    TAATTTCGAT TTAGATTAGG GAAAGGTTGA GACTTGAGAG AAGTTGCAGT
    TTCTACGCTT TTGAGGAGAC TGATTGGAAC AGCGTATGAA TCTAGAGTTA
    AACACTTGTC AGTTGTCAAA CTACTACAAA CTAATGAATC TGTACAGATG
    GGCTTGATG

    Gene models with high sequence identity

    1. AT1G05260.1 [+] show detail - Peroxidase superfamily protein
      E-value: 1e-75; Score: 280.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 4.6e-52
    superfamily Haem peroxidase 7.1e-94
    HMMPfam Haem peroxidase, plant/fungal/bacterial 1.8e-66
    ProfileScan Haem peroxidase, plant/fungal/bacterial 67.672
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Plant peroxidase 3.6e-45
    FPrintScan Haem peroxidase, plant/fungal/bacterial 2.3e-24
    FPrintScan Haem peroxidase, plant/fungal/bacterial 2.3e-24
    FPrintScan Haem peroxidase, plant/fungal/bacterial 2.3e-24
    FPrintScan Haem peroxidase, plant/fungal/bacterial 2.3e-24
    FPrintScan Haem peroxidase, plant/fungal/bacterial 2.3e-24

    See the detailed result >

    AT1G05260.1

    Model type
    Protein coding
    Short Description
    Peroxidase superfamily protein
    Curator Summary
    Encodes a cold-inducible cationic peroxidase that is involved in the stress response. In response to low temperature, RCI3 transcripts accumulate in the aerial part and in roots of etiolated seedlings but only in roots of light-grown seedlings.
    Computational Description
    RARE COLD INDUCIBLE GENE 3 (RCI3); FUNCTIONS IN: peroxidase activity; INVOLVED IN: response to desiccation, response to cold, hyperosmotic salinity response; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant peroxidase (InterPro:IPR000823), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: Peroxidase superfamily protein (TAIR:AT3G21770.1); Has 4433 Blast hits to 4402 proteins in 259 species: Archae - 0; Bacteria - 4; Metazoa - 3; Fungi - 76; Plants - 4304; Viruses - 0; Other Eukaryotes - 46 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR