K18024

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18024

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18024

Determination of introduced cDNA(s)

  • J033109N02 - Rice Full-length cDNAs - Original: J033109N02

    full reading [+] show sequence

    Nucleotide sequence: J033109N02 - full reading 
    GCATCCTGCA GACTCGCCCC ACTCTCTTGC TCAGCTCCTC TTCATCCAGT 
    CCTCAAAACT CTCTCTGATC TCTCTCTCTT GCTGAAAATT CTCCGCACAC 
    TCCGGTGGGC TTAAGCGGTG GCGGCGTCTC GCCTGGGTGG GCGAGATCCC 
    TCTCTTGCTC GTCCGCAAGG TCGCTCCCCC TGCGAGTCCA ATCTACTGAC 
    GAGCTGGGAG GAGCAAAGGA GGGGGCAATT GGAGCTCCGC TCGGTTCCAA 
    TTCAGCCCCA ATTTTGAGCC CCCCGGCTGC GGGGTTCGGC CAGTTGAGAC 
    GCCATGTATC GGGTGAAGAG CGAGAGCGAC TGCGATATGA TCCATCAGGA 
    GCAGATGGAC TCGCCGGTGG CCGACGACGG CAGCAGCGGG GGGTCGCCGC 
    ACCGCGGCGG CGGGCCCCCG CTGAAGAAGG GGCCATGGAC GTCGGCGGAG 
    GACGCCATCC TGGTGGACTA CGTGAAGAAG CACGGCGAGG GGAACTGGAA 
    CGCGGTGCAG AAGAACACCG GGCTGTTCCG GTGCGGCAAG AGCTGCCGCC 
    TCCGGTGGGC GAACCACCTG AGGCCCAACC TCAAGAAGGG GGCCTTCACC 
    GCCGAGGAGG AGAGGCTCAT CATCCAGCTC CACTCCAAGA TGGGGAACAA 
    GTGGGCTCGG ATGGCCGCTC ATTTGCCAGG GCGCACTGAT AATGAAATAA 
    AGAATTACTG GAATACTCGA ATAAAGAGAT GCCAGCGAGC TGGCCTACCC 
    ATCTATCCTA CCAGCGTATG CAATCAATCC TCAAATGAAG ATCAGCAGTG 
    CTCCAGTGAT TTTGACTGTG GCGAGAATTT GTCAAACGAT CTTCTGAATG 
    CAAATGGTCT TTACCTACCA GATTTTACCT GTGACAATTT CATTGCTAAT 
    TCAGAGGCTT TACCTTATGC ACCACATCTT TCAGCCGTTT CTATAAGCAA 
    TCTCCTTGGC CAGAGCTTTG CATCAAAAAG CTGTAGCTTC ATGGATCAGG 
    TAAACCAGAC AGGGATGCTA AAACAGTCTG ATGGTGTGCT TCCTGGATTG 
    AGCGATACCA TCAACGGTGT GATTTCCTCG GTGGATCAAT TCTCAAATGA 
    CTCTGAGAAG CTCAAGCAGG CTGTGGGTTT TGACTATCTC CATGAAGCCA 
    ACTCTACCAG CAAGATTATT GCGCCTTTCG GGGGTGCACT TAATGGCAGC 
    CATGCCTTTT TAAATGGCAA TTTCTCTGCT TCTAGGCCCA CAAGTGGTCC 
    TTTGAAGATG GAGCTCCCTT CACTCCAAGA TACTGAATCT GATCCAAACA 
    GCTGGCTCAA GTACACTGTA GCTCCTGCGT TGCAGCCTAC TGAGTTAGTT 
    GATCCCTACC TGCAGTCTCC AGCAGCAACC CCTTCAGTGA AATCAGAGTG 
    CGCGTCGCCA AGGAATAGTG GCCTTTTGGA AGAGTTGATT CATGAAGCTC 
    AGACCCTAAG ATCCGGGAAG AACCAACAGA CATCTGTGAT AAGTTCTAGT 
    TCTTCTGTCG GTACGCCATG TAATACTACG GTTCTTAGCC CAGAGTTTGA 
    TATGTGTCAG GAATACTGGG AAGAACAACA TCCTGGTCCA TTCCTCAATG 
    ACTGTGCTCC TTTCAGTGGC AATTCATTCA CTGAATCCAC CCCTCCTGTT 
    AGCGCTGCAT CGCCTGACAT CTTTCAGCTC TCCAAAGTTT CCCCAGCACA 
    AAGCACTTCA ATGGGATCTG GAGAGCAAGT AATGGGGCCT AAATATGAAC 
    CTGGGGACAC TTCACCTCAT CCTGAAAACT TCAGGCCAGA TGCATTGTTT 
    TCTGGGAATA CAGCTGATCC ATCAGTTTTC AACAATGCCA TAGCAATGCT 
    TCTGGGCAAT GACTTGAGTA TCGATTGCAG ACCTGTTCTT GGCGACGGTA 
    TCATGTTCAA TTCTTCCTCG TGGAGCAACA TGCCACACGC CTGTGAAATG 
    TCAGAATTCA AATGAATTCT GTATTTGGTA TTTGCCAGAC GCTACAGCAG 
    ATTCTTGGTA TCGTTCTTGC TCATTGTTTG GATCATAACT TGAGGAAGAT 
    CTCGTCGGTT GTAATTCTGC ATTGCTGACA GAGTCCTTGA TGGCATGCAG 
    GCACTATCAG ATGTTCTCTT ATGCGACTAG CCCTTTTGTC CAATAAAGTA 
    GTGCATGGAG ATAAGCCGGT TAATTATTGT TTTCTTTTTT GTATTAGAGA 
    ACCCTTTTTG TCATCTCTAT TGCCCGAATT CTGTTGGAAA TTCGTCATGT 
    TTGTTTGAAC AATGTAAGAC CAATTATTGC ATTTGTGGTG TACTATCTTG 
    TTGAGC

    Gene models with high sequence identity

    1. AT5G06100.3 [+] show detail - myb domain protein 33
      E-value: 4e-75; Score: 280.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Homeodomain-like 9.1e-23
    superfamily Homeodomain-like 9.4e-08
    HMMPfam Myb, DNA-binding 4.4e-14
    HMMPfam Myb, DNA-binding 8.7e-14
    Gene3D Homeodomain-related 1.8e-15
    Gene3D Homeodomain-related 4.2e-16
    HMMPanther R2R3-MYB TRANSCRIPTION FACTOR 1.5e-80
    HMMPanther Myb transcription factor 1.5e-80
    HMMPIR Transcription factor, GAMYB 0
    HMMSmart SANT, DNA-binding 2.1e-14
    HMMSmart SANT, DNA-binding 6.3e-17
    ProfileScan HTH transcriptional regulator, Myb-type, DNA-binding 25.026
    ProfileScan HTH transcriptional regulator, Myb-type, DNA-binding 16.625

    See the detailed result >

    AT5G06100.3

    Model type
    Protein coding
    Short Description
    myb domain protein 33
    Curator Summary
    Encodes a member of the myb family of transcription factors (MYB33), contains Pfam profile: PF00249 myb DNA-binding domain. Double mutants with MYB65 are male sterile- anthers are small, pollen development is defective. Spatial expression appears to be under the control of miR159, contains a target site for this micro RNA. When the target site is mutated , expression is detected in leaves, roots, anther filament, pistil. The expression of a translational fusion is specific to anther locules in contrast to constructs lacking the miR159 target site. Phenotype is conditional and can be restored by lower temperature or higher light intensity.
    Computational Description
    myb domain protein 33 (MYB33); CONTAINS InterPro DOMAIN/s: Transcription factor, GAMYB (InterPro:IPR016310), SANT, DNA-binding (InterPro:IPR001005), Myb, DNA-binding (InterPro:IPR014778), Homeodomain-like (InterPro:IPR009057), Myb transcription factor (InterPro:IPR015495), Homeodomain-related (InterPro:IPR012287), HTH transcriptional regulator, Myb-type, DNA-binding (InterPro:IPR017930); BEST Arabidopsis thaliana protein match is: myb domain protein 65 (TAIR:AT3G11440.1); Has 8918 Blast hits to 8315 proteins in 480 species: Archae - 0; Bacteria - 0; Metazoa - 726; Fungi - 466; Plants - 5984; Viruses - 3; Other Eukaryotes - 1739 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR