K18124

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18124

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18124

Determination of introduced cDNA(s)

  • J013052K11 - Rice Full-length cDNAs - Original: J013052K11

    full reading [+] show sequence

    Nucleotide sequence: J013052K11 - full reading 
    GACGTCCAAT TTCTTTCTCC AAGAAAGAAC GCCGAACAAG CGCCGACGAA 
    AAACGGTTCC TCGTTATTTC TCTCCAACGC GCGCGCACGG GCTATTTCTA 
    CCCCCGCCGC CGAGTAGTTA GGCGTCCAAA GTTTCGGGAG TTCATCGTGA 
    ACGGTGATCG ATCCAAAGAA CAACAACTCG GAGCAAAGCT AGCTAACGAA 
    GTTAGTTCCA AACAAAGCTA GCTTATCGCG ATTAGCTAGC TAGAAGAGAG 
    TTAGCTAGGC GCCACGCAGC TAGGCGTGCC ACTGCCACGA CGGCCCGTGC 
    CCGGTAGCTA CGGCGTGCCG TTCGTCTCGG CGGTGCGCGA CCGCCTCGAT 
    TTCTACTACT TGCAGGGGCA GGACAAGTAC TTCGAGTCGC GCGCCGAGAG 
    GTACGGCTCC ACCGTCGTCC GCATCAACGT CCCGCCTGGC CCATTCATGG 
    CGCGCGACCC CCGCGTGGTG GCGCTCCTCG ACGCCAAGAG CTTCCCCGTC 
    CTCTTCGACG TCGCCAAGGT CGAGAAGCGG GACGTGTTCA CCGGCACGTT 
    CATGCCGTCC ACCTCCCTCA CCGGCGGCTA CCGCGTCTGC GCCTACCTCG 
    ACCCGTCCGA GCCCAACCAC GCCAAGATCA AGCAGCTGCT CCTCTCCCTC 
    CTGGTCTCTC GCAAGGACGC CTTCGTCCCG GTCTTCCGCT CCAACTTCGG 
    CGCGCTCCTC GACACCGTCG AGTCGCAGCT CGCGAGCGGC GGCGGCAAGT 
    CCGACTTCAC CGCCCTCAAC GATGCCACCT CCTTCGAGTT CATCGGCGAG 
    GCGTACTTCG GCGTGCGTCC CTCCGCGTCG AGCTCCCTCG GCACCGGCGG 
    GCCGACCAAG GCCGCCCTGT GGCTCCTATG GCAGCTCGCC CCGCTCACCA 
    CGCTCGGCCT GCCCATGATC ATCGAGGATC CGCTCCTCCA CACGCTGCCG 
    CTGCCACCCT TCCTCATCAG CTCCGACTAC AAGGCGCTGT ACGCGTACTT 
    CGCCGCCGCG GCGTCGCAGG CGCTCGACGC CGCCGAGGGC CTTGGCCTGT 
    CGCGGGAGGA GGCCTGCCAC AACCTGCTGT TCGCGACGGT GTTCAACAGC 
    TACGGCGGCT TCAAGCTGCT GCTCCCGCAG ATCCTGGCGC GCGTCGCGCA 
    GGCCGGCGAG AAGCTCCACG AGAGGCTCGC CGCGGAGATA CGGAGCGCGG 
    TGGCCGACGC CGGCGGCAAC GTGACGCTGG CCGCTCTGGA GAAGATGGAG 
    CTGACCAGGT CGGTGGTGTG GGAGGCGCTG CGGCTGGACC CGCCGGTCAG 
    GTTCCAGTAC GGGCGCGCCA AGGCCGACCT GGAGATCGAG AGCCACGACG 
    CGTCGTTCGC GATCAAGAAG GGGGAGATGC TGTTCGGCTA CCAGCCGTGC 
    GCCACCAGGG ACCCGCGGGT GTTCGGCGCC ACGGCGAGGG AGTTCGTCGG 
    CGACCGGTTC GTCGGCGAGG AGGGGAGGAA GCTGCTGCAA TACGTGTACT 
    GGTCGAATGG GCGAGAGACG GAGAACCCTA GCGTTGACAA CAAGCAGTGC 
    CCCGGCAAGA ACCTGGTGGT GCTCGTCGGA AGGCTGTTGC TCGTCGAGCT 
    CTTCCTCCGG TACGACACCT TCACCGCCGA GGCCGGCAAA AAGGTGGTCA 
    TCACCGGGGT CACCAAAGCT TCAACCTCCG CCGTCAATCG TACTGCTTAA 
    GCCGGCCATC ACTTCAGGCG TGGGTCGTCG ATCATCCACT CAACCAGCTC 
    CGTCCTACGC ATATGCAATA AGAAATACTC GTACCTGAAT TCTTGCATGG 
    CTAAACACTA CTAATAAGTG TATGTATTCT GTTTATTTGT TCGATTCCAT 
    ATTTGTAATT TTGTTGTTAT TGTTTGCTGT TGAATTACGA TTGTGTATTT 
    TACAGGGCTG ACAGTGTCTT TAAACACCTC TAATATGTAT CGGATCAATC 
    TGCCCTAGTC ATGTCTAATT TGTG

    Gene models with high sequence identity

    1. AT5G42650.1 [+] show detail - allene oxide synthase
      E-value: 1e-122; Score: 437.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Cytochrome P450 1e-11
    superfamily Cytochrome P450 3.4e-49
    Gene3D Cytochrome P450 3e-33
    HMMPanther Cytochrome P450 9.8e-09
    Coil coiled-coil 0

    See the detailed result >

    AT5G42650.1

    Model type
    Protein coding
    Short Description
    allene oxide synthase
    Curator Summary
    Encodes a member of the cytochrome p450 CYP74 gene family that functions as an allene oxide synthase. This enzyme catalyzes dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway. It shows a dual catalytic activity, the major one being a 13-AOS but also expressing a 9-AOS activity.
    Computational Description
    allene oxide synthase (AOS); FUNCTIONS IN: hydro-lyase activity, allene oxide synthase activity, oxygen binding; INVOLVED IN: in 7 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128); BEST Arabidopsis thaliana protein match is: hydroperoxide lyase 1 (TAIR:AT4G15440.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR