K18148

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18148

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18148

Determination of introduced cDNA(s)

  • J013002G04 - Rice Full-length cDNAs - Original: J013002G04

    full reading [+] show sequence

    Nucleotide sequence: J013002G04 - full reading 
    GTCCGGAGAG GAAAGGAAAG CTCGGGATCG GAGCAGTAGT AGTACTAGGA 
    CGACTCAACG ACGACGATGC TTCGCCGCCG CGGCTTCTGC TGCTGCTCCG 
    GCGCACCCGC CGCCGCCGCC GCCGCCTTGC TCCTACTGGC CGTGGCGGCG 
    GCCGCCCCCC GCGCCGCCGG GTTCCACCTC GGGGGCGACG AGAGCGTCCT 
    CGTGCGGGGC ATGCTCGCCG CGATCCGCCG CGAGCAGGCC GAGGCGGAGG 
    ACGCGGCGCG CTTCGCCGTC GCGGAGTACA ACAAGAACCA GGGTGCTGAA 
    TTGGGATTTG CACGGATAGT GAAAGCCAAG CGTCAAGTTG TGACTGGTAC 
    CTTGCATGAC CTGATGTTGG AGGTAGTTGA TTCTGGAAAG AAAAGCCTGT 
    ATAGTGCAAA GGTATGGGTG AAGCCGTGGC TAGATTTTAA GGCAGTCGTC 
    GAGTTCCGTC ACGTTGGGGA CTCCCAGTCA CAGTCGGCCA CTGCTGCTGA 
    TGATAACGCT GGGCAAGATA CTGCTGATCC CACTGTGGCA TCAAGGAACG 
    ACCTGCACAA CACTGAGAAC AATAAAGTCT CTGTTGTCCT GTCAACCTTT 
    TCTCAGACAT ACTCGGTGTG AATCTAGCTC CATGGAGAGG ATATGGAAGA 
    CTTGAGCTTC TGAGAGCTAG CTGTCAGTAA TTTGTGAAGT AAAGTAGCTG 
    TTATCCTTTT GTGAAGTTTT CCCCACTGTT ATGGAATGAT GTCTAGATCG 
    TAATATGCCG TTGAGCAGAC ATGAGTTTGA CATCTGGAGT GTATATTTGT 
    TGCTGCAAAC TGCAAAGTGA ACACTCCCAT GTATATTCCA TACCTTTCGT 
    TCCCATGCAT GTATATAAGG CATTACTGCT ACCGTTGTAT GGTATACCCG 
    CTGCATGTGT TTGCATTT

    Gene models with high sequence identity

    1. AT3G12490.2 [+] show detail - cystatin B
      E-value: 2e-17; Score: 87.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Proteinase inhibitor I25, cystatin 8.3e-13
    HMMSmart Proteinase inhibitor I25, cystatin 1.2e-19
    BlastProDom Proteinase inhibitor I25, cystatin, conserved region 2e-33
    Gene3D no description 1.5e-27
    superfamily Cystatin/monellin 4.5e-22

    See the detailed result >

    AT3G12490.2

    Model type
    Protein coding
    Short Description
    cystatin B
    Curator Summary
    Encodes a protein with cysteine proteinase inhibitor activity. Overexpression increases tolerance to abiotic stressors (i.e.salt,osmitic, cold stress).
    Computational Description
    cystatin B (CYSB); FUNCTIONS IN: cysteine-type endopeptidase inhibitor activity, cobalt ion binding; INVOLVED IN: response to abiotic stimulus; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Proteinase inhibitor I25, cystatin, conserved site (InterPro:IPR018073), Proteinase inhibitor I25, cystatin, conserved region (InterPro:IPR020381), Proteinase inhibitor I25, cystatin (InterPro:IPR000010); BEST Arabidopsis thaliana protein match is: Cystatin/monellin family protein (TAIR:AT5G05110.1); Has 755 Blast hits to 732 proteins in 99 species: Archae - 0; Bacteria - 2; Metazoa - 28; Fungi - 0; Plants - 721; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR