K18533

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18533

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18533

Determination of introduced cDNA(s)

J013026J17 - Rice Full-length cDNAs - Original: J013026J17

full reading [+] show sequence

GGCGCTGGCC GTCGCAAGGC CGCCGCATAC TCTTCTCCCC TCTTTGACTC 
GTTTTGCGCC CAAGTCGATC CCCAACGTCT CCTCCTCGCT GGTTCGCCCG 
CCGCGCCGCG CCGATCTCCA TCCGTTCCAC GAGCAGCGGT GACCGGGTGC 
TGTCGTCCTG CTCCCCGCTT CCCTTCCGGC TCCGATTTGA GGCGCTAAAG 
TTTCCATTCC GTGTTACGAA AAGCTGCATT AGGACATGGA CATGGCATTG 
CCAATAGTGA ATGCCACCGC AGCGGTGCTT GCTCGTGTCT CAGCTGCATT 
CAATGCCCCT TTTGCCCGCG CAGTTGTCTT TGGGGTTCAT ATCGATGGGC 
ACCTGGTTGT TGAAGGGCTC CTTATAGCGG TCATAGTGTT TCAGCTTTCT 
AGGAAGAGCT ACAAACCGCC CAAGAAGCCA CTCAGTGAAA AGGAGATTGA 
CGAGCTATGT GATGAATGGG AGCCAGAACC TCTGTGTCCT CCGATCAAGG 
ATGGGGCCCG AATTGACACA CCAATGTTGG AAAGTGCTGC TGCTCCACAT 
ACAACCATTG ATGGGAAAGA AGTTATAAAC TTTGCATCAG CAAATTATCT 
TGGTTTGATT GGCAATGAGA AGATTATTGA TTCTTGTGTT GGTTCACTGG 
AGAAATATGG TGTTGGTTCT TGTGGTCCAC GTGGCTTTTA TGGAACAATT 
GATGTCCACC TTGACTGTGA GGCAAAAATA GCGAAGTTTC TTGGAACTCC 
AGACTCAATT CTGTATTCAT ATGGGATTTC TACAATATTT AGTGTGATAC 
CTGCCTTTTG TAAGAAAGGA GACATCATAG TTGCTGATGA AGGTGTGCAC 
TGGGCAGTGC AAAATGGTCT TCATCTGTCA AGAAGCACTG TTGTTTATTT 
TAAACACAAT GATATGGCTT CACTTGCAAA CACTCTGGAA AAACTTACAC 
GTGGAAATAA ACGAGCAGAA AAGATCAGAC GATACATTGT TGTAGAATCT 
ATCTACCAGA ATTCTGGTCA AATTGCTCCC TTGGATGAGA TTGTCAGATT 
GAAGGAGAAA TATCGATTCC GTGTTATTCT GGAGGAAAGC CATTCTTTTG 
GTGTGCTTGG CCAGTCTGGA CGAGGCCTTG CTGAACATTA TGGAGTTCCA 
ATTGACAAAA TTGATATTAT CACTGCTGGA ATGGGGAATG CATTAGCTAC 
TGATGGTGGC TTTTGTACAG GAAGTGTTAG AGTTGTTGAT CATCAGCGTC 
TAAGCAGTTC TGGCTATGTT TTCTCGGCTT CTCTGCCACC TTATCTTGCC 
AGCGCTGCGG TTTCTGCTGT CAGCTACCTG GAGGGGAACC CGTCAGTTCT 
TGCAGACCTG AGGAGCAACA TTTCTTTCCT GCATAAAGAA CTGTCAGGTA 
CTCCAGGACT TGAAATTTCC AGCCATGTTC TGTCACCTAT CGTTTTCCTC 
AAACTGAAGA AATCGACGGG TTCATCAAAC ACCGATATAG ATCTTCTTGA 
AACTATTGCT GAGAGGGTAT TGAAGGAAGA TTCAGTTTTT ATCGTGGCGT 
CAAAGCGGTC ACCTTTGGAT AGGTGCAAAC TTCCCGTCGG AATACGCCTC 
TTCATGTCAG CCGGCCACAC CGATTCAGAC ATCTCCAAAG TGTCCTCATC 
CTTGAAGCGG GTCTCAGCAT CAGTTCTGTC AGATTACATC TGATCCCCTT 
TCTCTACGAG CTGTGCACTA ATCTCTGTGT GCATGTTTGG TTAATGTACA 
TAACCTGTAC ATTTGTAGGT TCCGATGATT CCCGTTGCCG CTCATACTTG 
CAAACACTTC GAAAAATAGA CGTAGAACAG TGAAATTAGG TGTGTTATGT 
ATCGTTCGGT CTTCTGGTAG TGGTTTCCCG GTAGAATTCA TTCTATATCT 
TTAATTTTGT CCTCTATTCA TCACTTTTAT CATTTTGGCT TC

Gene models with high sequence identity

AT4G36480.2 [+] show detail - long-chain base1
E-value: 5e-180; Score: 628.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	3e-71
HMMPfam	Aminotransferase, class I/II	3e-40
superfamily	Pyridoxal phosphate-dependent transferase, major domain	2.7e-70
Coil	coiled-coil	0
HMMPanther	SERINE PALMITOYLTRANSFERASE I	1.4e-226
HMMPanther	CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANO	1.4e-226

See the detailed result >

AT4G36480.2

Model type: Protein coding
Short Description: long-chain base1
Curator Summary: Encodes the LCB1 subunit of serine palmitoyltransferase. Together with the LCB2 subunit, forms a functional serine palmitoyltransferase complex, which catalyzes the first reaction of sphingolipid biosynthesis. Knockout of LCB1 was embryo lethal. Partial suppression of LCB1 expression led to smaller plants due to reduced cell expansion.
Computational Description: long-chain base1 (LCB1); FUNCTIONS IN: protein binding, serine C-palmitoyltransferase activity; INVOLVED IN: cell growth, sphingolipid biosynthetic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR