K18547

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18547

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K18547

Determination of introduced cDNA(s)

  • J033097E23 - Rice Full-length cDNAs - Original: J033097E23

    full reading [+] show sequence

    Nucleotide sequence: J033097E23 - full reading 
    GAGCAACAGC AACAGCCCAG TACTACTAGC ACCGAGACGA GACGAGAGAG 
    AGACGGTCGC CCTCCCAGTC CAGTACAGTG ACTCACTCTC ACTCCAGACT 
    CCTCCCTCTC TCTCTCGTCT CTCTCCTGCC GGCGGCGCCG TGGCGGCGAG 
    CGGCGGAGCA CTAGGGGAGG AGGGGGCTCG TGCTCGTCTG TCGCTGGGTG 
    TGTCGGGGGC GCCGCGGATC ACTCTGCCTC GAGCTGCGCG CTCGCATTCC 
    GCTCCTTCGA TCTGGGTTCA TCTGTTCTGA GATGGCTGGC GACATTACGT 
    GTGGATCCTT GCTGCAGAAA CTGCAGTTGA TATGGGATGA AGTTGGTGAG 
    AGCGACGAGG ACCGCGACAA GGTCCTGTAC CAGTTAGATC AGGAGTGCCT 
    GGATGTTTAC AAGAGGAAAG TTGACCAGGC CACCGACTCT AGGGATCTCC 
    TTATCCAGGC GCTGGATGAC TCGAAGATTG AGCTGGCCAG ACTTTTATCT 
    GCTCTAGGAG AGAAGGCCAT AGCAAGAACT CCTGAGAAGA CGTCTGGGAC 
    AATCAAGCAA CAACTTGCTG CTATAGCGCC AACACTCGAG AAGTTGAACA 
    AGCAGAAAAA CGAAAGAGTG CGGGAATTTG TTAATGTTCA GTCACAAATT 
    GATCAAATAT GTGGTGAGAT TGCTGGCACT ACGGAGGTGG GTGAGAAGGT 
    GGCAACGCCA CAAGTTAATG AGGATGATTT GACACTTGAG AGGCTTGAAG 
    AATTTCGTTA ACAACTACAG GAGCTCGAAA AAGAGAAGAG CAATAGGTTG 
    GAGAAGGTTC TTGATTATGT GAGCATGATA CATAATCTCT GTACTGTCTT 
    AGGGATGGAT TTTCTTAGCA CAGTGACTGA AGTTCATCCC AGTTTGGATG 
    ACTCTATTGG TGACAACTGC AAGAGCATTA GCAATGATAC ACTGTCAAAA 
    CTTGACAAGA CTGTAGCCAC ACTTAATGAA GACAAAAAGT CAAGACTAAG 
    CAAGCTTCAA GAGCTTGCTG GTCAACTCTA CGATCTTTGG GATCTCATGG 
    ATGCCCCCAT GCAAAAAAGG AGCATGTTTG ATCATGTCAC CTGCAACAGA 
    TCAGCCTCTG TAGACAAAGT CACTGCACCT GGAGCTCTTG CCCTGGATCT 
    CATTGAGCAA GCTGAGGTAG AGGTTCAAAG GCTAGACCAG CTAAAATACA 
    GCAAGATGAA GGAAATAGCT TTCAAGAAGC AAACTGAGTT GGAAGACATC 
    TATGCTGGTG CTCACATGGT AATAGATACA GCTGCTGCCC ATGAAAAAAT 
    ATTGGCGCTG ATTGAGGCAG GGAACATAGA ACCATCAGAA TTGATTGCAG 
    ATATGGAAAG CCAGATATCA AAAGCAAAGG AAGAAGCATT GAGTAGAAAA 
    GAAATATTAG ACAAAGTTGA AAGATGGATG TCTGCTTGTG AAGAAGTAAG 
    CTGGCTTGAA GATTATAACC GGGATGACAA CAGATATAAC TCAAGCCGAG 
    GTGCCCACCT GAATCTCAAA CGTGCTGAAA AGGCTCGGAT TCTTGTCAAC 
    AAGATTCCAG CACTTGTTGA AACTCTGGTG GCAAAGACCA GGGCATGGGA 
    GGAGAGCCGT GGTCTGTCCT TTATGTATGA TGGTGTACCT CTTCTAGCTA 
    TGTTGGATGA ATATGTCATG CTAAGGCAGG AAAGGGAAGA AGATAAGAAA 
    AGAATGAGGG AACAAAAGCG CTACATCGAG CAACAACTGA ACACCGACCA 
    TGAAGGGCCA TTTGGTTCAC GTGTGAGCCC TAACAGACCA GTCAGTGCAA 
    AGAAGGTACC TGGTGCAAAA TCAAATGGCA GTGCTAATGG CACTCCTCCG 
    AATAGGAGAC TTTCGGTCAG CGGTCACCAG AACGGAAGGT CTGGAGGAAA 
    GGATGGCAAG AGGGACTCTG CCAAGACAGC TTCCCCCGGG AATGTAGCAG 
    CTGCTAAGGA AGATGCATCC TCACACATTT CTGGCACCGA TCCGGTTCCG 
    AGCACACCAT GATCTTTAAC CTTATGGTAT ATTAGATAAA GAGAGGACAG 
    TTTCTGATGT TTCCATTGAT TTGTGGCTAT ATAATGTATT GTGTTTGTAG 
    CGAGGAGTAA TTATATAACG TCCATCTTTC AGGCAGGATC TTTTGCTTCT 
    GTGGAGTAAG TGGAGTTCAA CTTTTGATTA GTTCCCTCAG CTGTACTATT 
    TCCCCTTCTC ATCAGACTCT GTAGTATAGC CTTTGCTGGT ATGTATTACT 
    ATTCGTTAAC CTGTTTGCTA CATGTACAAG TTCTTGGAAT GCCTTTCCCC 
    TGCCC

    Gene models with high sequence identity

    1. AT5G55230.1 [+] show detail - microtubule-associated proteins 65-1
      E-value: 0; Score: 681.00

     

    InterPro Scan Digest

    Program Description E-value
    Coil coiled-coil 0
    HMMPanther Microtubule-associated protein, MAP65/ASE1-type 3.2e-111
    HMMPfam Microtubule-associated protein, MAP65/ASE1-type 1.8e-59

    See the detailed result >

    AT5G55230.1

    Model type
    Protein coding
    Short Description
    microtubule-associated proteins 65-1
    Curator Summary
    Binds and bundles microtubules. Plays a role in stabilizing anti-parallel microtubules in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. The timing with which the MAP65-1 was targeted to the spindle appears to be regulated by a phosphorylation sensitive switch. Enhances microtubule polymerization, promotes nucleation and stabilizes microtubules against cold treatment and dilution.
    Computational Description
    microtubule-associated proteins 65-1 (MAP65-1); FUNCTIONS IN: microtubule binding; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Microtubule-associated protein, MAP65/ASE1-type (InterPro:IPR007145); BEST Arabidopsis thaliana protein match is: microtubule-associated protein 65-2 (TAIR:AT4G26760.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR