K18824

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K18824

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)

Invisible phenotypes

Original: K18824

Determination of introduced cDNA(s)

  • J013038G02 - Rice Full-length cDNAs - Original: J013038G02

    full reading [+] show sequence

    Nucleotide sequence: J013038G02 - full reading
    GATATCTAGA AAAACAGAAA AAGAATATAA GGGGGAAGAC TTGGGCCGCC 
    GCTAACCCTA ACCCCTGGCG ACGGCGGCCG ATCGTCGTCG ACGAAGTCAG
    GCGCGGAGGC TGCGACCGGA TTAGCCGGTC GCCCCTGGAT TTGGAGCTCC
    CGTGTCCGTG TGGGGGCAAT GCTAGCGCGG GCGTTTGGCC TCGTGGGCAA
    AACATGGAAG CGAACGCCGA CTATGTCCGT CGAGGCTTCC GCCTCGCCGC
    GCCGCCGCTC CCAGATGCCA CAGCAGCAGC TTTGTCATCT CCACGGCAGC
    ACGCCGTCAC AACGGCAAAA GTTCCCCGAA GAACAGCTCC CGGGAGGAGC
    TCCGCCAATG CTGGACTATA TCTCCGAACA GAACTTAAGC ACCGTAGTAA
    TGGCAACATG GAGAATTCAG CTGTTGCTGC TGTCGACTCT AATGGATAAC
    CTTGCTCGCT TCTGAACTTC AATATTGAAC TAAGCTTTGT AAGGTTGGCA
    TGCTAAATTA GTTGTTTACA TTCAGGGATA CTGTTGAGGG GAGTTTTGAC
    CTGAGGTTTG CAGCACTGAA GAGCATTGGG GAGGATCGGG TCAACGATCG
    TGAGCTTAAG TTACTGCTAA AGAAGAAACA TGCCCCCATT TGTTATGTTT
    GGTGTGATCC ATGCCCCTGG ATGCACATTT CTCAGGTACA ATATTGAGAT
    GTGGAAAGCA ACTGGCATGG CTCTTGATAA AGTAGATCTA GTCTGGTTGT
    CAGATGAAAT ACGTCAACAT GGTGATGAGT ACTGGCCAAT TGTCATGGAT
    ATTGCAAGGA CAAACAGTGT GCGTAGAATA AAAAGTACAT GCAGGTCCTC
    TTATTACTGT TTACCTAGCA GATTTTGTGG GAGTAGAAAT TCTTATGCGA
    TTGGAAAATT AAATGTTGAT GAGATTTTTT ACATTTGCTT GCAGTGTGCT
    AGCATATTAT TTCAGAAGGC AGACATATGG CTGTTGGGAA TGGAACAGCA
    TGATGCCAAC TTATTAGCTA GACAATATTA TAAGCATTTC AAAAAGAAGA
    ATAAGGCAAT TGCTGTTTTG GACAATATGC TTCCTAATTT ATTACAATAC
    CCTCAAATGG AGAACAGGAG GCATCCAGCT TGGGCTATCT TCATGGAAGA
    TGATAAGGAG GATATATGTT TTAAAATGGA AAAAGCTTTC TGCCCTCCAA
    AACTAGCAGA AGGCAATCCA TGTTTGGAGT ACATAAAATA TATCATCTTG
    CCTTGGTTTG GAATGTTTGA GGTGGTCCAG AAGAAAGGGA ATGGTGGCAA
    AAAGACTTTT CTGAGCATGG AGGAACTTAC AGCTGATTAT GAAAGTGGCG
    CTCTGCATCC TGCTGATGTG AAGCTGGCCC TTGAAAAGTC ATTGAACGAG
    ATATTGCAGC CTGTTCGTGA TCACTTTGGA AGGAATGGTG AAGCAAAGGA
    TCTAGTGGAA GCTATCGGGG AATGCTACGG TGCTGACTGA TATTACATGT
    TCACTCTTGG ACTTAGGAGT AGTGTTCTTA TCTTTGGCCA TGTAATGAAC
    AAATCAATCC CTATCTATGA GCAGTCTTAG AGGTTCCTTT TGTGCCACAA
    GGTCATCAGT TCAGTGCTTG CTCTTTCCTC GTGGGTGTGT TAGAAGGGTG
    TCTTTTTTTT TCCTCATCAC AACCTTGTTT CTTGTATTTT TTACCTGCTA
    TATGAATATG AAACCTCCCG CTGTCTCGTG CGGTTAGTTC CC

    Gene models with high sequence identity

    1. AT2G33840.1 [+] show detail - Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial
      E-value: 8e-65; Score: 245.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Aminoacyl-tRNA synthetase, class Ib 5e-21
    HMMPIR Tyrosine tRNA ligase, archaeal/eukaryotic 7.7e-114
    Gene3D Rossmann-like alpha/beta/alpha sandwich fold 2.9e-13
    Gene3D no description 1e-32
    superfamily Nucleotidylyl transferase 5.6e-41
    HMMPanther Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic 4.9e-39
    HMMPanther ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHION 4.9e-39

    See the detailed result >

    AT2G33840.1

    Model type
    Protein coding
    Short Description
    Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial
    Computational Description
    Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial; FUNCTIONS IN: tyrosine-tRNA ligase activity, nucleotide binding, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: translation, tRNA aminoacylation for protein translation, tyrosyl-tRNA aminoacylation; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Tyrosine tRNA ligase, archaeal/eukaryotic (InterPro:IPR016485), Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic (InterPro:IPR015624), Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (InterPro:IPR002307), Aminoacyl-tRNA synthetase, class Ib (InterPro:IPR002305); BEST Arabidopsis thaliana protein match is: Nucleotidylyl transferase superfamily protein (TAIR:AT1G28350.1); Has 5443 Blast hits to 5419 proteins in 1791 species: Archae - 389; Bacteria - 3121; Metazoa - 323; Fungi - 286; Plants - 124; Viruses - 5; Other Eukaryotes - 1195 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR