K19251

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K19251

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
petal (PO:0009032)
  • abnormal (PATO:0000460)
    (Flower::Shape::petal)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K19251

Determination of introduced cDNA(s)

  • J033116E13 - Rice Full-length cDNAs - Original: J033116E13

    full reading [+] show sequence

    Nucleotide sequence: J033116E13 - full reading
    GGTTGGCTTC CAGAAGCTTC TAGAAGGTGC CCGAGGCGTC GTCTCGGTCG 
    AGCGCCATGG ATCTGGACCC CGAGGGCCTC TTCCGCGACG ACAGCGACGA
    GGATGACGAC AACGTCCAGG AGAGGGAGGC CAACAAGGAG ATGGTCGTCT
    ACCTCATCGA CGCCTCGCCC AAGATGTTCA CCCCTGCGAC CAAGGCAGAT
    GAAAAGGAGG AGACACATTT TCATACGATA GTGAACTGCA TCACACATGC
    GCTGAAGACG CAGATTATCG GTAGATCCTA TGATGAAGTC GCTATATGTT
    TCTTTAACAC GAAAGAAAAG AAGAATTTAC AGGAATTGGC TGGTGTTTAT
    GTTTATAATG TTACAGAGAG AGAACCACTT GATAGGCCTG ATGCAAGATT
    GATTAAGGAA TTTTCTTGTA TAGAAGATTC TTTTATGAGT AATATTGGAA
    GTCGGTACGG AATAACTTCT GGATCTCGAG AGAATACCTT GTACAATGCT
    CTTTGGGTTG CACAGGCATT GCTGCGTAAA GGATCTGTGA AGACTGTGAG
    TAAGCGGATT GTTATATTCA CCAACGAAGA TGATCCTTTT GGTGGTCTTA
    CAGGAGCAGT AAAGACTGAT ATGATAAGGA CAACAATCCA ACGTGCAAGA
    GATGCACAAG ATCTTGGCCT GTCCATTGAA CTTCTTCCTT TGAGTAGGCC
    CGATGAGGAG TTCAACATGT CCCTGTTTTA TGCAGATTTA ATTGGTCTGG
    AGGGAGATGA AATAGTGGAT TATCTGCCAT CTTCTGGTGA AAAGCTGGAG
    GATATGACTA ATCAGCTGAA AAAACGAATG ATGAAGAAAC GCAAAGTCAA
    AACTCTTGCA TTTGCAATTA CCAATGATGT TTGCATAGAG GTGAATACAT
    ATGCGCTAAT CCGTTCAACT ACTCCAGGGG CAATTACATG GCTTGATTCT
    ATCAGCAACC TGCCATTGAA GGCTGAAAGG TCTTTCATAT GCAATGACAC
    TGGGGCCCTT ATTCAGGATC CACAAAAGCG CTTCCAGGTG TACAATGAGT
    AAGTACACAC ATGAGACTGG TGAACCAAAG CGGTGTTATT TTTGTATAAT
    TTTCTGGGTT GGGTTCTGTG ATCTGCTTCT TGATTGCTTC ATCAAGCGCA
    GAAGTCTACA GTTATGAGTC ATGGCACGAT CATTGTCTCG TCATTCCGTC
    ATGTTGTTCC TTTTCCGTTT CAGTTTGATG CTTATATTTG TAATCCAAAA
    CTACTTTATC ATATATTCAT GCACTTTTTT GCACAGCAAA ATTGTTAAAT
    TCTCTACTCG TGAATTGTCT GATGTGAAGA GGGTTTCAAG TCATCATCTA
    CGCCTTTTAG GATTCAAGCC ACTGGACTAC CTTAAAGATT ATCACAACTT
    AAGACCGTCA ACATTTATTT ACCCCAGTGA TGAGGTAATA ACATTACTTC
    ATGTACTTCT TAACTTCAAT CTTGAGGCCC TCTTGGCACA GCTCCGGATC
    CAAGATTTCT TGGAGCTGGG TATTCCGAGC TCCACAAATC CATGGATCTG
    GATCTGTGAT TACGTTGATA AACATTTGGA TAAATCATCT TGTTCCCATT
    TAAAATTTTA AATCACTATA ATTTAATACA CAAACTTCAA ATCTACCGTT
    TGAGTTACAG TTTGAAGTTA TACTAGTACA ATTATCTCCT TCATCTTAAT
    TTTTTTTTGT CAAACACGCA GGAGAGCTGC GTAGTCTTGA CAATATTTTG
    TTATCTTGTT CGTTTGTTCC CTAGATTATT GTACTTCTCT AGCTTGAAGC
    AATAATATAA TAGTTGAAAA CCTTAATCAT TTTATTTGAT GATATTATTT
    TTCATAATAA AAGATCTATT TGTGTTCGAT ACACATCCCT GCTGCATGCT
    ACTTCCAAAC TAAACTAAAT CTGTCTGAAC ATATTTAGGA TTGTATTGTG
    GTGTATGCTT ATTTCTTTCT CAGTCTTTAC TTAAATCTAG GGTGCATGTT
    AGGTTTACCT CCTTTTGTTT GATGCAGCAA ATATTTGGAA GCACTCGTGT
    TTTCGTTGCT TTACATAGCT CAATGCGGCG TCTTGGAAGG TTAAGTCGAC
    TTATTATATT TCTGCCATAA TTTCTTTAAT TTTGAAAGGG TTTAGAAACT
    GACATAGTTA TAAATGGACA TAATGTATGT CAAGTCATAA AATGTACTGT
    GGATTAAATT AATTAACATT ACTGCTCATT TTTTGGGATA ATTAGTGAGG
    GGATGCAATT GAAAGGGGAT GTTAGTTTTG TTGAAATATC ACTCTATGCG
    ATAGTTATCT CAAATTACAG CTCACTAGTA ATATGCCCGT GCTAAATGCT
    ACGGTCCAAT TTTATTTGTC TTTAATAATT TTCAAAACGT CATGTTAGCC

    Gene models with high sequence identity

    1. AT1G16970.1 [+] show detail - KU70 homolog
      E-value: 2e-111; Score: 400.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam DNA helicase, ATP-dependent, Ku type 5e-10
    HMMPfam Ku70/Ku80, N-terminal alpha/beta 2.7e-50
    HMMPanther KU P70 DNA HELICASE 1.2e-29
    HMMPanther KU AUTOANTIGEN DNA HELICASE 1.2e-29
    HMMPanther KU P70 DNA HELICASE 1.1e-10
    HMMPanther KU AUTOANTIGEN DNA HELICASE 1.1e-10
    superfamily Spen Paralogue and Orthologue SPOC, C-terminal-like 1.1e-09
    superfamily vWA-like 1.4e-56
    superfamily Spen Paralogue and Orthologue SPOC, C-terminal-like 2.4e-07
    Gene3D no description 5.8e-09
    Gene3D no description 3.1e-61

    See the detailed result >

    AT1G16970.1

    Model type
    Protein coding
    Short Description
    KU70 homolog
    Curator Summary
    Ku80 and ku70 form the heterodimer complex Ku, required for proper maintenance of the telomeric C strand. Ku regulates the extension of the telomeric G strand. Interacts with WEX, and this interaction stimulates the WEX exonuclease activity.
    Computational Description
    KU70 homolog (KU70); FUNCTIONS IN: double-stranded DNA binding, protein binding; INVOLVED IN: DNA repair, response to heat, telomere maintenance; LOCATED IN: nucleus, DNA-dependent protein kinase-DNA ligase 4 complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ku70/Ku80, N-terminal alpha/beta (InterPro:IPR005161), DNA-binding SAP (InterPro:IPR003034), DNA helicase, ATP-dependent, Ku type (InterPro:IPR006164), Spen Paralogue and Orthologue SPOC, C-terminal-like (InterPro:IPR016194), Ku70/Ku80 C-terminal arm (InterPro:IPR005160), DNA helicase, ATP-dependent, Ku70 subunit (InterPro:IPR006165); Has 475 Blast hits to 466 proteins in 181 species: Archae - 0; Bacteria - 6; Metazoa - 182; Fungi - 177; Plants - 52; Viruses - 0; Other Eukaryotes - 58 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR