K19527

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K19527

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Elements::Others::K

Original: K19527

Determination of introduced cDNA(s)

J033030G03 - Rice Full-length cDNAs - Original: J033030G03

full reading [+] show sequence

GAATTAGCTC GTTCTCTTGT GTCAAGAAAA AAAAAGAAAG AAAAAGCTCG 
CCGCCGCCGC CACCGTCGCC GGCGCGATGA TCACCGGACG CGACATCTAC 
GACGTGCTGG CGGCGATCGT GCCGCTGTAC GTGGCGATGT TCCTGGCGTA 
CGGGTCGGTG CGGTGGTGGG GGATATTCAC GCCGGACCAG TGCTCCGGCA 
TCAACCGCTT CGTCGCCGTC TTCGCCGTGC CGCTCCTCTC CTTCCACTTC 
ATCTCCACCA ACGACCCCTA CTCCATGAAC TACCGCTTCC TCGCCGCCGA 
CTCGCTCCAG AAGCTCGTCA TCCTCGCCGC GCTCGCCGTC TGGCACAACC 
TGCTCTCCCG CTACCGCCGC AATGGCGGCG CCGCCGCGTC GCTCGACTGG 
ACCATCACCC TCTTCTCGCT GTCCACGCTG CCCAACACGC TGGTCATGGG 
CATCCCGCTG CTCCGCGCCA TGTACGGCGA CTTCTCCGGC TCGCTCATGG 
TGCAGATCGT CGTGCTCCAG AGCGTCATCT GGTACACCCT CATGCTCTTC 
CTCTTCGAGT ACCGCGGCGC CAAGGCGCTC ATCTCCGAGC AGTTCCCGCC 
GGACGTCGGC GCCAGCATCG CCTCGTTCCG CGTCGACTCC GACGTCGTCT 
CGCTCAACGG GAGGGAGGCG CTGCAGGCGG ACGCCGAGGT GGGGCGCGAC 
GGCCGCGTCC ACGTCGTCAT CCGCCGCTCC GCCTCGGCCT CCACCACGGG 
CGGCGGCGGC GGCGCGGCGC GCTCCGGCGT GTCCCGGGCG TACGGCGCGT 
CCAACGCCAT GACGCCGCGC GCCTCCAACC TCACCGGCGT GGAGATCTAC 
TCGCTGCAGA CGTCGCGCGA GCCCACGCCG CGGGCGTCCA GCTTCAACCA 
GGCCGACTTC TACGCCATGT TCTCCGGCAG CAAGATGGCC AGCCAGATGG 
CTAGCCCCAT GGCGCAGCAC GGCGGCGCCG GCGGCCGCGC CCAGGGCCTC 
GACGAGCAGG TCACCAACAA GTTCGCCTCC GGCAAGGCCG CCGACCCGCC 
GTCGTATCCC GCCCCGAACC CCGGCATGAT GCCGGCGCCA AGGAAGAAGG 
AGCTCGGGGG CTCAAACTCC AACTCCAACA AGGAGCTACA CATGTTCGTG 
TGGAGCTCTA GCGCGTCGCC GGTGTCGGAG GCCAACCTCC GCAACGCCGT 
CAACCACGCC GCCTCCACCG ACTTCGCCTC CGCGCCGCCG CCGGCAGCCG 
TTCCCGTCGG CGGCGCCACT CCCAAAGGGG TGAGTGGCAG TGTCACGCCG 
GCGGCGAAGA ACGGCGGCGG CGAGTTGGAG ATCGAGGACG GGCTGAAGAG 
CCCGGCGGCG GGGCTGGCGG CGAAGTTCCC GGTGTCGGGG TCGCCGTACG 
TGGCGCCGAG GAAGAAGGGC GGCGGCGCCG ACGTGCCCGG GCTGGCGGAG 
GCGGCGCACC CGATGCCGCC GACGAGCGTG ATGACGCGGC TCATCCTCAT 
CATGGTGTGG CGCAAGCTCA TCAGAAACCC CAACACCTAC TCCAGCCTCA 
TCGGCCTCGT CTGGTCCCTC GTCTCCTTCA GGTGGAATAT CCAAATGCCT 
TCAATAATAA AGGGCTCAAT ATCAATATTG TCAGATGCAG GGCTAGGAAT 
GGCTATGTTC AGCTTAGGCT TGTTCATGGC TCTGCAACCA AAGATCATTT 
CTTGTGGCAA GACCGTTGCG ACATTTGCAA TGGCAGTGAG GTTCTTGACT 
GGTCCAGCTG TTATTGCAGC TACTTCCATT GCCATTGGGC TCAGGGGAGT 
ACTCTTGCAT GTTGCCATTG TTCAGGCAGC ACTTCCACAA GGCATTGTCC 
CGTTTGTGTT TGCCAAGGAG TACAATTGCC ATCCTCAAAT ACTTAGCACA 
GCGGTTATTT TTGGGATGCT CATCGCGCTT CCGATCACGA TACTCTACTA 
TGTGCTTCTT GGGATATAGT GTTCTTGAAG AAGGCAAAAA AGAAAGAGTA 
GGGAAAAAAA TAGGATTCTA GGTTTCTAGA GGAAAATGCA AAAGAAATAT 
GATATGGGCT TTCTTGAAGA CCTGAAGAAC TACCAGAGCT GAAGAATAGG 
GAAATGAGAT CAAGTAGGAT CCTAGCTAGA GAGAAATGCA AAGGAAAGAC 
ACCCCTTGAT TACAATTTTT TAATTTTTTC TGCAACTGTT TTGGCATCAA 
AGTAAAGGTT AGGGCCTTGA GTATGAAGAG TTCAGCCGTT AATTTGACAA 
GTTGGGTTGG CGGTACTAAA GATTCC

Gene models with high sequence identity

AT5G57090.1 [+] show detail - Auxin efflux carrier family protein
E-value: 0; Score: 726.00

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Auxin efflux carrier, subgroup	8.8e-267
HMMPfam	Auxin efflux carrier	8.1e-164

See the detailed result >

AT5G57090.1

Model type: Protein coding
Short Description: Auxin efflux carrier family protein
Curator Summary: Encodes an auxin efflux carrier that is similar to bacterial membrane transporters. Root-specific role in the transport of auxin. Acts downstream of CTR1 and ethylene biosynthesis, in the same pathway as EIN2 and AUX1, and independent from EIN3 and EIN5/AIN1 pathway. In the root, the protein localizes apically in epidermal and lateral root cap cells and predominantly basally in cortical cells. Functions may be regulated by phosphorylation status. EIR1 expression is induced by brassinolide treatment in the brassinosteroid-insensitive br1 mutant. Gravistimulation resulted in asymmetric PIN2 distribution, with more protein degraded at the upper side of the gravistimulated root. Protein turnover is affected by the proteasome and by endosomal cycling. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. Membrane sterol composition is essential for the acquisition of PIN2 polarity.
Computational Description: ETHYLENE INSENSITIVE ROOT 1 (EIR1); CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT2G01420.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR