K20003

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20003

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20003

Determination of introduced cDNA(s)

  • J033073L16 - Rice Full-length cDNAs - Original: J033073L16

    full reading [+] show sequence

    Nucleotide sequence: J033073L16 - full reading 
    GGCGGCGGAC GGAGGGAGGG ACGGCGCCGC TCGGGAGAGG GAGCGGGAGC 
    GCGTGCGTCG CCGGTGGCCG AGCCGAGCCG AGCTGCGTCG CGGTGGTGCT 
    CTCCGCGGGC GGCGAGATTC AGCCCTGACC CGAGGAAGGT GAGGGGAATT 
    CTTCCTCGGG GGTGGGTTTG GTGCATTCCG TGGTGTGAGA TGCGGGGGAT 
    GAACAAGGCT TTCGGGGTTT GGGATGTCGC GGGGATTTGG GGGCGGTGGG 
    TGGTTTTCGG CTGGGAATTT GCGGATTTCG TGGCTGCTAG TGGTGTTCCC 
    GTGGCTTTAC GAACAGATGT ATATGCTTGA TCTCGTGCGG CTTTCCCTTC 
    TATCCCTCTC TCCATGGTTT GCTCGCGTTG GGGGGTTTCG AAAAATTTTC 
    CCTACTGTTA GTCCTCGATT CTGGTCGTCG GCATGGTGGC GGTCGCCTCC 
    GCCTCCCCGC TCACCGCCGC GGCGGCGCCC TCCATGTGCT GCTCGCCGCC 
    CGCCTTCCTC CCTCTCCGTG CGCACCGCAG GAGGATTTTC CGCGCGGAGG 
    CGATGAAGAC GCAGCAGGAG AAGAAGCAGA CCGAGGTGGC CGTCGAGGAG 
    TCCTTCCCCT TCAGGGAGAC GGCTCCTCCT GATGAGCCGC TGGTCACCGC 
    CGAGGAGAGC TGGGTGGTTA AGCTCGAACA GTCTGTGAAC ATTTTCCTCA 
    CGGAGTCAGT CATCACGATA CTTGATGGAT TGTACCGTGA CCGCAACTAC 
    GCTAGATTTT TCGTCTTGGA GACGATTGCT AGGGTGCCGT ATTTTGGTGA 
    GGATCTCGTC GTACATGCTC GCTATAGCCT GCAGCGTTTA TCTCTATGCT 
    TCACATGTAT GAAACATTTG GCTGGTGGAG GAGAGCTGAT TATATAAAGG 
    TGCACTTTGC TGAGAGCTGG AATGAGTTCC ATCACCTCTT AATCATGGAA 
    GTACGTCTCT AGCTCTTTCT GTCTGCACAT CAGTGCATTT ATATATGCTG 
    TTTCTTCCTG TTTTGATGTC CTTTTAGATT ATGTTAGCTG TTAGCATATC 
    CCGTTTTCTT TAACTGAATA TAATCTTTAA AGGAATTCAA CCTACTGCTT 
    ATTGACACAT TATCTGTTTT TTTTTTAATA TTTTGGTTAT GGTCTTTGAT 
    GTTCTGACTT TATTCCTCAT CTTGGTTCAT ATTAGGAATT GGGTGGCAAC 
    TCTTTATGAG TTGATCGTTT TCTTGCATGG TTTGCTGCAT TTTTTTACTA 
    CTTCATGACT GTTGCGATGT CACGAGCCCC GGCGTAAGAA CGTATGGGAT 
    ATGAATGTGT GATTGGCGGT GGACGAGGGG GTTTGGTGGA ACGACGACCC 
    GTGATGAACA GCAAGGGATG AGCAAATAGA GAATAGGAGA TGAGAGAACT 
    AGAGTTTGAA TCTGCTTAAT TAAACGCCAC CGAAACTGTG CCTCTAAATA 
    GGCGTACACG AATTAACCAA TGTGCTAAAA ACAAGCTAAC AAACTGATAA 
    ACAGACTAAC AATCTGAACT CATCCAATCG CCGCACTGTA GTCATCCGGC 
    ACGCAGCTTC CGCGCGCTAC TCCGCCGTTC CGCCCGGCGA CGCTTGCGGA 
    ACCACACGCC CTCTGCGACG ATACGTAATC CCGACCATGA CATGCGATGT 
    ACATGGTGAG CCCAAGAATG GCATGTAAGT GTGCACCTAT GCCAATTATG 
    AAGTAAAACA CTTCATTTTT ATTTCCATGC CTTTTATGTA ATGATAACGC 
    TTGGAATTGT TTATCTTATA TACATACCGC TTTTCTGAAT GTGTTGAGAG 
    GCATGCGTAC TCAACCTATG ACAAGTTTAT TAAGCTCCAT GAAGGTAATA 
    AAACGTTGAT GGATTTGACA TCTCTCCTGT CGTGCAAAAT TTTATCATGA 
    CAACTAGACA TCTCAAAATT TTTGTTGCTT TATCTAGGTT GGACAGACTT 
    TGTAAACCAA AGTGGAAATG AGGGGACCGG GAAAACTAAG TATTTACTTT 
    TTTTATAGGG ATAAGTTTAT GCCTACCTTT TGTCACAGAT TGCCCTTGTG 
    TGAATAATTT GATACATGCG CCAGTGAACT TATGTTATAT CAGCTATTTA 
    CTTCAATTAT TGGCAATCAG GATTCAGGCA TGGTGTTTAT TTTAGTTTTA 
    TAGGTATCGT GTGCATAAAT TCAAAAACAT GTATTATTCT TCCATTTTTG 
    ATTAAGATGT CGGCTCTGGT ATCCTTTTAT GATGTACTAT ATTTAGATTT 
    TTTTTCCGTC CTACATAGTG GTTACTTGAA TTGTGGGAGA GTTGAAACAT 
    ACCTGAAAAA ATTAGCAAAG CTAGATCCCC TAAGGTCTGA ACACCAAACT 
    GGTAGACTCT TTGCATTACT ATCCATTCAG AAGTTGATGC ATTGTACATT 
    TTAAAGGGTG CATTTTAATA AACCAAAATG TTGAACACTG CAACCAATAA 
    CACCATGTCT ACCCGACCTG TCCACCAACT AGAAATTTAC AGGAACATCA 
    CACTCTGTCA ATTATATTTA CGTATTTCTT GGCAAAATAA CATCCTGATC 
    TGACCTTTGA CTGCAGAAAT GTAGCTGATG CCATGCCTGC ATGCCACCCA 
    TGAGTGGCAT ACCTAATATC AATATCAAGG AATGACTTCC TGTAGTGCTC 
    TTCTTCCTTG AGAGCACACG CAATAAGCTA TCAAAGAGTA TACAGCTTTT 
    CCAAGAAAAC GAAAGGGAAC GACAGAGAAT TGCCACTCTC TAACCCAAGA 
    AATATCACAG TTGCAAGATC TTGGCAGCAC ACTTATTGAG TTGTGGAACC 
    TAATGGACAC ACCAACTGCT GAACAGAAAT GCTTTGACCA TGTTACGTCT 
    TTGATTTCAG TCTCACCAAA TACAAAAATG CCCCAAGGAT GCCTTGCACG 
    TGAACTTATT GAGAAGTTTA CAATTGATCG ACCATTTTAT GTTCTTTTGC 
    TTTATTTGGA TTGTCATTGA AGCTAACTTA ATTTTTCTGT AAAATCCGGT 
    TGAGGTTAAG AGGTTGAATT CTTCTGATCA TTGTCAATAC AACTATAGCG 
    ATTCATTGAG GGTAAGAGCT CACATAACTT TTATGTGCTT TACT

    Gene models with high sequence identity

    1. AT4G22260.1 [+] show detail - Alternative oxidase family protein
      E-value: 5e-39; Score: 85.10

     

    InterPro Scan Digest

    See the detailed result >

    AT4G22260.1

    Model type
    Protein coding
    Short Description
    Alternative oxidase family protein
    Curator Summary
    Similar to mitochondrial alternative oxidase. im mutants have a variegated phenotype and fail to differentiate chloroplasts in the majority of their cells under high light intensity continuous illumination. The white tissues of immutans accumulate phytoene, a non-colored C40 carotenoid intermediate. This suggests that immutans controls, either directly or indirectly, the activity of phytoene desaturase (PDS), the enzyme that converts phytoene to zeta-carotene in higher plants. However, im is not the structural gene for PDS. It is located in the lumenar face of the thylakoid membrane. IM is expressed ubiquitously in plant tissues.
    Computational Description
    IMMUTANS (IM); CONTAINS InterPro DOMAIN/s: Alternative oxidase (InterPro:IPR002680); BEST Arabidopsis thaliana protein match is: alternative oxidase 2 (TAIR:AT5G64210.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR