K20539

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20539

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20539

Determination of introduced cDNA(s)

  • J013108N11 - Rice Full-length cDNAs - Original: J013108N11

    full reading [+] show sequence

    Nucleotide sequence: J013108N11 - full reading
    GATCTCTCCT TTTTTTTTTC TCCCCAAATC ACCCCTCCGC CGAAACGGGA 
    GCTCGATCTC CGCCGCCACC GCCAATCCCC GAACCCTACC TCTCCCCCCC
    TCCACCCATG GCGCTCTCCC GCCGCCTCCT CCCGCTCCTC CTCCGCCGCG
    GGTCCACCCC CTCCCTCTCC CCCGCCCGCG CGCTCTCCAC GGCCGCCGTG
    ACGGCCGATG CTCCCGCCGC GGCGGCGGCG GCGGCGGAGG AGGAGGCGAT
    GACCATCAAG GGCGTGCGCA TCTCGGGGCG GCCGCTGTAC ATGGACATGC
    AGGCGACCAC CCCCGTCGAC CCGCGGGTGC TCGACGCCAT GCTCCCCTTC
    TACCTCTCCC GCTACGGGAA CCCCCACTCC CGCACCCACC TCTACGGCTG
    GGAGTCCGAC GCCGCCGTCG AGGAGGCCCG CGCCCGCGTC GCCTCCCTCG
    TCGGCGCCGA CCCCAGGGAG ATCTTCTTCA CCTCCGGCGC CACCGAGTGC
    AACAACATCG CCGTCAAGGG CGTCATGCGC TTCTACCGCG ACCGCCGCCG
    CCACGTCGTC ACCACGCAGA CCGAGCACAA GTGCGTCCTC GACTCCTGCA
    GGTACCTCCA GCAGGAAGGC TTCGAGGTCA CCTACCTCCC CGTGCGCCCC
    GACGGCCTCG TCGACGTCGC GCAGCTCGCC GACGCCATCC GCCCCGACAC
    CGGCCTCGTC TCCGTCATGG CCGTCAACAA CGAGATCGGC GTCGTGCAGC
    CACTCGAGGA GATTGGTCGA ATCTGCAAGG AGAAGGGCGT GCCCTTCCAT
    ACTGATGCTG CCCAGGCGCT CGGGAAGATC CCGATTGATG TCAACCAAAT
    GGGGATTGGG CTCATGTCTT TGTCTGCGCA CAAGATTTAT GGGCCAAAGG
    GCGTGGGTGC TCTCTATCTG CGTCGTCGAC CCCGCATTCG TGTGGAGCCG
    CAGATGAGTG GCGGTGGCCA GGAACGCGGA ATTCGCAGTG GAACAGTGCC
    AACGCCTCTT GTTGTTGGAT TTGGTGCTGC CTGCGAAATT GCGGCTAAAG
    AGATGGACTA TGATCACAGG AGAGCCTCGG TTCTGCAGCA GCGCCTTCTT
    GATGGCATCC GTGGACAGGT TGATGATATT GTTATCAATG GAAGCATGGA
    GCATCGGTAC CCTGGTAACC TGAATTTGTC ATTTGCATAT GTTGAGGGGG
    AGAGCTTGCT GATGGGCCTG AAGGAGGTGG CTGTGTCGAG TGGCAGTGCC
    TGCACTAGTG CCAGCTTGGA GCCCTCTTAT GTGCTGCGGG CACTTGGTGT
    AGAAGAGGAC ATGGCGCACA CCTCCATTCG CTTTGGCATT GGCCGCTTTA
    CCACTGAGGA AGAGGTTGAC AGAGCAATTG AGCTCACCGT GCATCAGGTG
    AAGAAGCTTC GTGACATGAG TCCACTGTAT GAGATGGCCA AGGCAGGCAT
    TGACCTCAAG AGTATCCAGT GGGCGCAGCA CTGAGACAGC TTTCACTTAG
    AGCTAATCCC TGGCTGCTTT TGAAGTGCAC GGAAGGACAC ATAGATAGGC
    CTGTCTGGTG TTGGTGAAGA CATTTCTATG AACCATGTGA TAGTTCTGTC
    AAGACAATCA GCTAGTATTG TACATCTGTT CACTTGCCAA GTTGCCAGCT
    CTCAGGCTGG CAACTAATGT TTTCTGCTAG GTTAGCACCT TGTTCTCTAG
    CTAGAATACC CCAACACGAA ATAAGAATGC TTGAGTGGTT ACTGTGTTGC
    CTGAAACCCT TTAAGATACT AATTGGAGTT ACTGGCATAA TGCTGTTACT
    ATATATTTTG GTGCTC

    Gene models with high sequence identity

    1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
      E-value: 0; Score: 633.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 5.4e-95
    PatternScan Aminotransferase class-V pyridoxal-phosphate binding site 0
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1.1e-115
    HAMAP Cysteine desulfurase 53.764
    HMMPanther CYSTEINE DESULFURYLASE 1.4e-159
    HMMPfam Aminotransferase, class V/Cysteine desulfurase 2.2e-87
    FPrintScan PRICHEXTENSN 0.00000000009
    FPrintScan PRICHEXTENSN 0.00000000009
    FPrintScan PRICHEXTENSN 0.00000000009
    FPrintScan PRICHEXTENSN 0.00000000009

    See the detailed result >

    AT5G65720.1

    Model type
    Protein coding
    Short Description
    nitrogen fixation S (NIFS)-like 1
    Curator Summary
    cysteine desulfurase whose activity is dependent on AtSufE activation.
    Computational Description
    nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR