K20542

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20542

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
T2  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20542

Determination of introduced cDNA(s)

  • J033050O03 - Rice Full-length cDNAs - Original: J033050O03

    full reading [+] show sequence

    Nucleotide sequence: J033050O03 - full reading 
    GGGCCGTCGT CTCCTCCCCC CGACCCCGCC GCCGCCGACC TCTCTCCTCC 
    GCGCCGCCGA CGAACCACCG CCGCCGAATC CCCCTCGCCG GCGGCCACCC 
    GGATCTCCCC AGCCATGTAC GGCGGCGATG AGGTCTCGGC CATCGTCATC 
    GACGTGGGGT CCTACAGCTG CAAGGCGGGG TACGCCGGTG ATGACACCCC 
    CAAGGCCGTC TTCCCCTCGG TTGTTGGTTC AATTGAGCAA ACGGGAGAAA 
    CTGATGAAGC AAAGGCAGAC AAGGAGGCTG AAGCTGCATC AGATTCTAAG 
    AATGGAGCCA AACCTATGGA TGTGGACAAA GCCAAGACAA AGCGCAAATT 
    GTATGTTGGT CAAGAATTGG AGTTCAGGAG GGATCATATG GAGGTGATTT 
    CACCAATGAA AGATGGAACA GTTACTGATT GGGATATTGT TGACAATATA 
    TGGAATCATG CTTTCAGGCA GCGGCTATTG ATCAATCCTG AGGAGCATCC 
    TATGTTGATA GCTGAACCAT CTACAAACAC AGGACAGCAA AGAGAGAAAG 
    CAGCAGAACT TATGTTTGAG AAGTACAAAG TGCCGGCACT GTTCCTAGCG 
    AAAAATGCAG TTCTCACATC TTTTGCATCT GGACGTGCTA CATCGTTGGT 
    AGTTGACAGC GGTGGTGGGT CTACTGTGGT TGCTGCTGTT CACGATGGTT 
    ATGTATTGCA AAAGTCTGTG GCGACTTCTC CAATTGGTGG TGAATTTTTA 
    ACTGACTGTA TGATGAAAAG CTTGGAGAGC AAGGGCGTTG TTATAAGGCC 
    CAGGTATTCA TTCAAGAAGA AGGAAGTCGG CCCTGGCGAA TATAAGGTTG 
    TAGATCTTGA TCTTCCAAAC ACAACGGAGA GTTACAAGTT ATACTGCATG 
    AGAGCTATTG CTAGTGATAT CAAGGAGTCA GTTTGCAGGG TTCCAGATAC 
    TGCCTTCGAT GAAGTGGCAT ATGCAAACGT TCCTACAACT TCATATGAGC 
    TTCCAGATGG ACAAACCATT GAAGTTGGTG CAGACAGATT CAAGATTCCT 
    GATATTCTGT TTAATCCATC TCTTTCTCAG ACTATTCCTG GAGTTGATGG 
    ATTTGCAGAT TCAATGTCAG TTCGTGGCCT TCCACGAATG GTCATTGATA 
    GTGTAAATAG GTGCGATGTT GACATTCGCA AGGAACTGCT AAGCAGTATT 
    CTGCTTTCAG GTGGTTCATC ATCAATTCTG CAGTTGAAGG AGAGGCTAGA 
    AAAAGAAGTA CTTGAGGAAT CTTCTGGTAA TACTCGTGTA AAGGTTTTGG 
    CAAGTGGAAA TTCAGTAGAG AGGCGATTCA GTGTTTGGAT TGGAGGGAGC 
    ATTCTAGCAT CCCTTGGGTC GTTCCAGCAA ATGTGGTTCT CCAAAGCAGA 
    ATACGAAGAA CATGGAGTAT CCTACATCCA AAGGAAGTGC CCATGAAGTT 
    GTGGAGCATC GCTACGATTT GTGACCAAAT TTGGGTGCAT GTGGGCAATC 
    GTATTACAGC CACCCTGTTG TTGATCTATA TCGACTATTA TCCGACGATA 
    TTTATCATTA TATTATGACT AGTTAGTTTG TAGATTTTGA GAGGGCAACA 
    TAAGAAGCAA TCCAGCTTAA CCTGTTATGT TCTTGATGGT AGATTCTAGT 
    TCATGTGTTG AATCTGTTCT CCCTGCTGTA GAATGTATCG AGTTGCTGCT 
    CTCTACTCTG TACTTTTAGA ATACCTTTTC AATCATTTGG AGTCAGCTGA 
    TTGTTGTACC

    Gene models with high sequence identity

    1. AT1G18450.1 [+] show detail - actin-related protein 4
      E-value: 3e-169; Score: 592.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Actin/actin-like 1.4e-117
    Gene3D no description 4.6e-47
    Gene3D no description 0.0000000000000012
    HMMPanther ACTIN-LIKE PROTEIN 6, ACL6 1.4e-184
    HMMPanther Actin/actin-like 1.4e-184
    HMMPfam Actin/actin-like 7.6e-147
    superfamily Actin-like ATPase domain 1.3e-64
    superfamily Actin-like ATPase domain 6.5e-50

    See the detailed result >

    AT1G18450.1

    Model type
    Protein coding
    Short Description
    actin-related protein 4
    Curator Summary
    Encodes a gene similar to actin-related proteins in other organisms. Member of nuclear ARP family of genes. Component of chromatin remodeling complexes, involved in chromatin-mediated gene regulation. Phenotype of the arp4-1 mutant allele revealed partial sterility due to defects in anther development. Targeting the distinct, 3' UTR of AtARP4 transcripts with RNA interference caused a drastic reduction in the level of AtARP4 protein expression, and resulted in strong pleiotropic phenotypes such as altered organization of plant organs, early flowering, delayed flower senescence and high levels of sterility. Western blot analysis and immunolabelling demonstrated a clear correlation between reductions in the level of AtARP4 expression and severity of the phenotypes.
    Computational Description
    actin-related protein 4 (ARP4); FUNCTIONS IN: structural constituent of cytoskeleton; INVOLVED IN: long-day photoperiodism, flowering, chromatin organization, pollen sperm cell differentiation; LOCATED IN: nucleolus, nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Actin/actin-like (InterPro:IPR004000); BEST Arabidopsis thaliana protein match is: actin-11 (TAIR:AT3G12110.1); Has 13669 Blast hits to 13362 proteins in 2792 species: Archae - 2; Bacteria - 480; Metazoa - 5593; Fungi - 3430; Plants - 1457; Viruses - 2; Other Eukaryotes - 2705 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR