K20826

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20826

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20826

Determination of introduced cDNA(s)

  • J013041C03 - Rice Full-length cDNAs - Original: J013041C03

    full reading [+] show sequence

    Nucleotide sequence: J013041C03 - full reading 
    GCCAAGAGAC CACATACCCC CAACGACCCT CCTCCCCTCC CCCCTCCTCT 
    CCCCCTCGCC GAGATATATT TTCCCCTCGG CGGCGACTCC TCCTCCACCT 
    CGTGAGCTCG CCTTCGGCCG CCGCCTCTGC GCCTCGCAGA TCCCCACCCG 
    CGACCGAGCG AGCTGGTGCC GCATCGGTAT CGCATCCCGT TCATCTTCTG 
    GCTGGACGAA TTCAATTCAC GGACGGTGGT GGTGGTGGTG TTGACTAGGG 
    ATTTATTTTT TTACCATTGG CGGCTCGGCT GCATCCCCAA GTGAAGAAGA 
    GAATTCGTAC ATGATTCCGC TAAACAGGAG GGCGAGTCAG ACTAGGGGTG 
    GGATGGAGTA CTTCGATGCT AGGCGGAAGC CTCATAATGT TGGGAAAGTT 
    ATTGCAGCTC TGGTCCTCAC AACACTATGT ATATTTATTT TGAAGCAATC 
    TCCTGGTTTT GGTGGCAGTA GTGTGTTTTC TCGCCATGAA CCTGGTGTTA 
    CTCATGTCTT AGTAACAGGA GGAGCTGGCT ATATTGGGTC ACATGCTTCA 
    TTACGCCTGT TAAAGGACAA TTATCGAGTT ACCATTGTGG ACAATCTTTC 
    TAGAGGAAAC ATGGGAGCAG TAAAGGTTCT CCAAGAGTTG TTCCCCCAGC 
    CAGGAAGACT TCAGTTTATC TATGCTGATC TTGGAGATCA AAAAACTGTC 
    AACAAGATCT TTGCCGAAAA TGCGTTTGAT GCTGTTATGC ACTTTGCTGC 
    TGTTGCCTAT GTGGGTGAGA GTACATTGGA ACCCCTTAGG TACTATCACA 
    ATATTACATC GAACACCTTA TTGATTTTGG AGGCTATGGC GTCTCATGGA 
    GTCAAGACCC TTATTTACTC TAGTACATGT GCCACCTATG GAGAACCAGA 
    GAAGATGCCT ATTGTAGAAA CCACACGTCA GTTACCAATT AATCCCTATG 
    GAAAAGCTAA GAAAATGGCG GAGGACATCA TACTAGATTT CACGAAGGGG 
    AGAAAGGACA TGGCTGTGAT GATTTTAAGA TATTTCAATG TTATTGGATC 
    AGACCCAGAG GGAAGACTAG GTGAAGCTCC TAGACCTGAA CTACGAGAGC 
    ATGGAAGGAT ATCCGGGGCA TGCTTTGATG CTGCGTTAGG AATCATTCCA 
    GGGCTGAAGG TTAAAGGAAC AGATTATCCT ACAACTGATG GAACCTGCAT 
    AAGAGACTAC ATTGATGTCA CAGATCTTGT TGATGCTCAT GTGAAAGCAC 
    TCAACAAGGC AGAGCCAAGT AAAGTTGGCA TTTACAATGT TGGCACTGGA 
    AGAGGTCGTT CAGTCAAGGA GTTTGTGGAT GCCTGCAAAA AGGCAACTGG 
    AGTCAACATT AAAATTGAGT ACCTCAGCAG GAGGCCAGGG GACTATGCTG 
    AAGTATACAG TGACCCAACA AAGATCAACA CGGAGCTCAA CTGGACTGCT 
    CAATATACCG ACCTCAAGGA GAGCCTGTCA GTCGCTTGGA GATGGCAAAA 
    GTCACATCCA CGCGGATATG GGTCAAACTA AATATAGCTT GCTGATCAAT 
    AATTCTGCTG AATCGTCGAT ATATTGATTT TTTTAGTTAG TGGTATAGGA 
    TCTTGGTGCG ATACCGCTTC CACAGTCACT CATTTGTTAT TTTTTTCTCT 
    TTTTATTTTC AAAGGTTTGT TGTTATTTTC TAGCAAAACC ATAAGCAGTG 
    ATCTTGATAT GCTGGAAATC CTGGAAGCCT TCCTGTAAGG GCAAATTTCA 
    TTGTTTTTTG ACACAAATTG CTG

    Gene models with high sequence identity

    1. AT1G30620.2 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
      E-value: 0; Score: 649.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily NAD(P)-binding domain 5.2e-89
    Gene3D NAD(P)-binding domain 3.3e-56
    HMMPfam NAD-dependent epimerase/dehydratase 2.3e-48
    HMMPanther UDP-glucose 4-epimerase 6.1e-150
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 6.1e-150
    HMMTigr UDP-glucose 4-epimerase 4.6e-142

    See the detailed result >

    AT1G30620.2

    Model type
    Protein coding
    Short Description
    NAD(P)-binding Rossmann-fold superfamily protein
    Curator Summary
    encodes a type-II membrane protein that catalyzes 4-epimerization of UDP-D-Xylose to UDP-L-Arabinose in vitro, the nucleotide sugar used by glycosyltransferases in the arabinosylation of cell wall polysaccharides and wall-resident proteoglycans.
    Computational Description
    MURUS 4 (MUR4); FUNCTIONS IN: UDP-arabinose 4-epimerase activity, catalytic activity; INVOLVED IN: plant-type cell wall biogenesis, arabinose biosynthetic process, nucleotide-sugar metabolic process; LOCATED IN: Golgi apparatus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040), UDP-glucose 4-epimerase (InterPro:IPR005886); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G20460.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR