K20924

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20924

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20924

Determination of introduced cDNA(s)

  • J023028A22 - Rice Full-length cDNAs - Original: J023028A22

    full reading [+] show sequence

    Nucleotide sequence: J023028A22 - full reading 
    GATAAAAAAA TTGGAAAAAG AGAAATAGAA AAGGAAGCGC ACCACCGCTC 
    TCGGCTCGCC TTCCCTTGGT TCCCGCACCT TCTCCTCCGC ATCGAGGCAA 
    ATCAATCAAT CCAATCCATC CCACACACCG CCCATCTCCC TGGCGGGCAG 
    CCTCGGCCTC GCGAGGGAGG GGAGGAGGAG GAGAGTGGCG GCGGCGGCGG 
    CGCGCCATGG CGTTCCTGCT CGGCGCCTTC CTCGGGCTGG TGCTCGGCGT 
    CGCCGTTGTC ATGGCGTTCG CGCGCCTCGA GAACACCCGC GCTGAGCAGC 
    GCCGCGAGCT GGCTGCTACT GTTTCATCCT TCTCAAAGTT GACTGTAGAA 
    GATTTAAGGA AACTAATTCC TCTTGAGTTA TATCCATCAT GGGTGTCTTT 
    CACTCAAAAG CAAAAGCTTA AATGGCTGAA TCAAGAATTG GTAAAAATCT 
    GGCCATTTGT CAATGAGGCT GCATCAGAAC TGATAAAAAC TTCTGTGGAG 
    CCTATTTTTG AGCAGTACAA GTCATTCATT TTGTCCTCAC TCCATTTCTC 
    AAAGTTGACA CTTGGAACAG TTGCCCCTCA GTTTACAGGA GTTTCAATTT 
    TGGATAGTGA TAGTTCTGGT ATCACTATGG AGCTTGAGTT GCAATGGGAT 
    GGCAATCCAA ATATAGTACT GGACATTCAA ACAACACTTG GAATCTCACT 
    TCCTGTGCAG GTGAAAAATA TTGGATTCAC AGGAGTACTT CGCTTAGTTT 
    TTAAGCCTCT GGTTGCAGAA CTGCCTTGCT TTGGAGCTGT TTGCTGTTCT 
    TTGAGAGAGA AGAGCAAGGT CGATTTTACC CTCAAGGTTA TTGGTGGTGA 
    GATGACAGCA ATTCCTGGAA TTTCTGATGC AATTGAGGGA ACGATACGTG 
    ATACCATTGA GGATCAGTTG ACATGGCCTA ATCGCATAGT TGTACCTATT 
    GTGCCTGGAG ATTATAGTGA CTTGGAGCTG AAACCAGTTG GACTATTAGA 
    AGTAAAACTT GTGGAAGCTA GGGACTTGAC GAACAAGGAT CTTGTGGGGA 
    AGTCTGACCC TTTTGCTGTG TTATACATTC GGCCATTGCA AGATAAAATG 
    AAGAAAAGCA AAACAATAAA TAATGATTTG AACCCCATCT GGAATGAGCA 
    CTACGAATTT GTGGTCGAGG ACACATCTAC CCAGCGCTTG ACTGTGAAAA 
    TTTATGATGA TGAAGGACTC CAGGCATCTG AGCTTATTGG CTGTGCTAGA 
    GTGGACTTGT CTGATCTTCA GCCTGGAAAA GTCAAAGAAG TTTGGTTGGA 
    CCTTGTCAAG GACCTGGAAA TCCAGCGGGA TAAGAAACGT CGCGGGCAGG 
    TACACCTGGA GCTCCTGTAT TACCCTTTTG GTAAACAGGA AGGGGTTTCC 
    AATCCTTTTG CAGATCAGAT TCAGTTAACT TCTTTGGAGA AGGTCCTCAA 
    GACTGAGTCT AACGGATTTG ATGTTAACCA GAGGAAAAAT GTTATTATGA 
    GAGGAGTCCT ATCAGTAACT GTTATCTCAG CAGAGGACTT GCCACCAATG 
    GATGTGATGG GCAAGGCTGA CCCATTTGTA GTCCTGTACC TGAAGAAGGG 
    AGAAACCAAA AAGAAGACAA GGGTTGTAAC TGAGACACTG AATCCAATAT 
    GGAATCAGAC GTTTGATTTT GTTGTTGAAG ATGCCTTGCA TGATTTACTC 
    ATGGTGGAAG TATGGGATCA TGATACGTTT GGAAAGGATT ACATAGGGAG 
    ATGCATCTTG ACACTTACGA GAGTAATACT CGAAGGCGAG TTTCAGGATG 
    AGTTTGTTTT GCAAGGTGCT AAATCCGGAA AGCTGAACTT GCACTTCAAG 
    TGGACGCCGC AGCCAATTTA TCGTGATCGT GATCGGGACC AGTGAAATCT 
    GTGTAGATAC ATCACGTCAA GCAGCTGATA AATATTTCTT CCCCTACGGT 
    GCCTGTACAT TAGTTTTTCT GTACAGTTAA TTCACTTATC TTGTAGCAAC 
    AAGGTGTAAT GGAATAAAAG AATGGTGGTT TCATGTTCCG ACGGAAATGC 
    GATGTGCTGT GAATGGAGGG TTGCAGAAAG ATGGATGGTA ACACGGGGAG 
    ATGGGTTACC CAAGTATGGA AACATTAAGT TCATCATGCA AGCAGATACT 
    TTATGCATCT TCTACTGGTA AAACTAAACG TGTTTGTCTT TAAAATGCAA 
    CTTGTAATAT AATTCCTTGT ACTAACAATT ACTACCTCCG TCCCATAATA 
    ATTGC

    Gene models with high sequence identity

    1. AT1G05500.1 [+] show detail - Calcium-dependent lipid-binding (CaLB domain) family protein
      E-value: 0; Score: 761.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart C2 calcium-dependent membrane targeting 2.6e-23
    HMMSmart C2 calcium-dependent membrane targeting 6.1e-25
    HMMPanther CALCIUM LIPID BINDING PROTEIN 5e-178
    HMMPanther CALCIUM-DEPENDENT LIPID-BINDING PROTEIN (CALB 5e-178
    Gene3D no description 7.1e-29
    Gene3D no description 2.5e-33
    superfamily C2 calcium/lipid-binding domain, CaLB 1.1e-35
    superfamily C2 calcium/lipid-binding domain, CaLB 1.9e-34
    FPrintScan C2 region 0.00000066
    FPrintScan C2 region 0.00000066
    FPrintScan C2 region 0.00000066
    ProfileScan C2 membrane targeting protein 15.118
    ProfileScan C2 membrane targeting protein 17.131
    HMMPfam C2 calcium-dependent membrane targeting 8.8e-22
    HMMPfam C2 calcium-dependent membrane targeting 2e-25

    See the detailed result >

    AT1G05500.1

    Model type
    Protein coding
    Short Description
    Calcium-dependent lipid-binding (CaLB domain) family protein
    Curator Summary
    Encodes a endomembrane-localized synaptotagmin. Synaptotagmin family proteins are calcium sensors that regulate exocytosis in mammalian cells.
    Computational Description
    NTMC2T2.1; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system, plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: C2 membrane targeting protein (InterPro:IPR018029), C2 calcium/lipid-binding domain, CaLB (InterPro:IPR008973), C2 region (InterPro:IPR020477), C2 calcium-dependent membrane targeting (InterPro:IPR000008); BEST Arabidopsis thaliana protein match is: Calcium-dependent lipid-binding (CaLB domain) family protein (TAIR:AT5G11100.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR