K21031

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K21031

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21031

Determination of introduced cDNA(s)

  • J013068N08 - Rice Full-length cDNAs - Original: J013068N08

    full reading [+] show sequence

    Nucleotide sequence: J013068N08 - full reading 
    GGCGAGTTGT CGCTTTTCCT CCCCAACTCC ACGATCGAGT GAAGCGGACG 
    AGGGCGACGG CGGCGAGCGC GCGCACAGCG TGTGATCCAT GGAGGCGTTC 
    GTGCCGGGCG GCGCGGGGGC GGCGGCGGCG GCGGTCGGAG GGTTCGTCGC 
    CGCGGCCGCG CTAGCCGAGA GGGCCGGCGT GATCGCGCCA AGGAAACGCC 
    CCAACGCGCC CCCAGCTGTT CCTGGTTTAC CCATTATCGG AAATCTGCAT 
    CAACTGAAAG AAAAGAAGCC TCATCAGACC TTTGCAAAAT GGGCTGAAAT 
    TTACGGGCCA ATCTACACTA TAAGAACCGG GGCTTCTTCT GTAGTTGTGC 
    TCAATTCAAC TGAAGTAGCC AAGGAGGCGA TGGTTGCAAA ATTCTCATCC 
    ATATCTACCC GAAAGCTATC CAAAGCACTG ACAGTGCTTA CTCGTGATAA 
    ATCCATGGTT GCTACCAGCG ACTATTGTGA TTTCCACAAA ATGGTGAAGC 
    GTTATGTCAT GTCAAGCATG TTGGGTACTT CTGCACAGAA ACAATTTCGT 
    GACATAAGAG ATATGATGAT CCATAACATG TTAAGCACTT TTCATAAACT 
    GGTGAAAGAT GACCCACATG CTCCTCTGAT ATTCAGAGAT GTTTTCAAGG 
    ATGAGCTATT CCGGTTGTCC ATGATCCAGA GCTTAGGAGA GGATGTGAGT 
    TCAGTCTATG TGGATGAATT TGGGAGGGAC ATTTCGAAGG AAGAAATCTA 
    CAATGCTACT GTGACCGACA TGATGATGTG CGCAATTGAG GTCGACTGGA 
    GAGATTTCTT CCCCTACCTC AGCTGGGTTC CAAACAAGAG CTTCGAAACA 
    AGAGTGTTTA CTACAGAAAC TAGACGAACT GCGGTGATGC GCGCCTTGAT 
    CAAGCAGCAG AAGGAAAGGA TTGTGCGTGG AGAGGCAAAG ACATGCTATC 
    TGGACTTCTT GCTGGCAGAG AACACACTGA CAGATGAGCA ACTGATGATG 
    CTAGTGTGGG AGGCACTCAT AGAGGCTGCA GATACTACCT TGGTCACCAC 
    AGAATGGGCC ATGTATGAGC TTGCCAAGAA CCCTGACAAA CAGGAACGGC 
    TTTACCAAGA GATCCGGGAG GTGTGCGGCG ACGAGACGGT CACCGAGGAG 
    CACCTCCCAC GGCTGCCGTA CCTCAACGCC GTCTTCCACG AGACGCTGCG 
    CCGCCACTCC CCTGTCCCGC TCATACCTCC GAGGTTCGTC CACGAGGACA 
    CCAAGCTCGC TGGCTACGAC GTCCCCGCCG GCACCGAGAT GGTGGTCAAC 
    CTGTACGGGT GCAACATGAA CAGGAAGGAG TGGGAGTCGC CGGAGGAGTG 
    GGTGCCGGAG AGGTTCGCCG GCGGGAGGCT CGAGGTGGCG GACATGTACA 
    AGACGATGGC GTTCGGCGCC GGGAGGAGGG CCTGCGCGGG GAGCCTGCAG 
    GCGACTCACA TCGCGTGCGC CGCCGTCGCG CGCTTCGTGC AGGAGTTCGG 
    GTGGAGGCTG AGGGAGGGTG ACGAGGAGAA GGTGGACACC GTGCAGCTCA 
    CCGCCTACAA GCTCCACCCG CTTCATGTCC ACCTCACGCG CAGAGGAAGG 
    ATGTGAACCA TTATATTCAT TGGAAGCTGT TGCTCGTTGA ATAATGATAT 
    ATAGTTATCT TTGAGATGTA TTTACTATTA GCAAAATCTC CATGCTTTGT 
    TATGGATAGG AGAAAACATA TTATAATATA TCATGGACTA TATTTCTTTT 
    ATCAAATATG GTTGTAT

    Gene models with high sequence identity

    1. AT5G25900.1 [+] show detail - GA requiring 3
      E-value: 3e-157; Score: 552.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther gb def: Ent-kaurene oxidase (EC 1.14.13.78) ( 2.4e-203
    HMMPanther Cytochrome P450 2.4e-203
    superfamily Cytochrome P450 1e-105
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, E-class, group I 1.9e-21
    FPrintScan Cytochrome P450, C-terminal 1.8e-05
    FPrintScan Cytochrome P450, C-terminal 1.8e-05
    FPrintScan Cytochrome P450, C-terminal 1.8e-05
    HMMPfam Cytochrome P450 1.8e-79
    PatternScan Cytochrome P450, conserved site 0
    Gene3D Cytochrome P450 4.4e-95

    See the detailed result >

    AT5G25900.1

    Model type
    Protein coding
    Short Description
    GA requiring 3
    Curator Summary
    Encodes a member of the CYP701A cytochrome p450 family that is involved in later steps of the gibberellin biosynthetic pathway.
    Computational Description
    GA requiring 3 (GA3); FUNCTIONS IN: oxygen binding; INVOLVED IN: gibberellin biosynthetic process, gibberellic acid mediated signaling pathway, ent-kaurene oxidation to kaurenoic acid; LOCATED IN: chloroplast outer membrane, endoplasmic reticulum, microsome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G07990.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR