K21135

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide) abnormal (PATO:0000460) (Rosette leaf::Others)
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21135

Determination of introduced cDNA(s)

J013092P04 - Rice Full-length cDNAs - Original: J013092P04

full reading [+] show sequence

AGCTCTCCCC ATCTATCTCC TCTCCCTCTC CGCCCATTTC TCCTCTCCAC 
CTGCGATCCA TCCGCGCCCG CGAGGAGGAG GGAGCCGGAC GCCGCCGCGG 
GGAGAGCAGG GGAGAGAAGG AGAGGAGGAG ATCGCCGTCG CCGTCGCCGT 
CGCCGTCGCC GATCTCGAGC GGGAGCGGAG ATGGGGCTCA CGTTCACGAA 
GCTGTTCAGC CGCCTCTTCG CCAAGAAGGA GATGAGGATC CTCATGGTCG 
GTCTCGATGC GGCCGGTAAA ACCACCATCC TCTACAAGCT CAAGCTCGGC 
GAGATCGTCA CCACTATCCC CACCATCGGT TTTAATGTCG AAACTGTTGA 
GTACAAGAAC ATTAGCTTCA CCGTTTGGGA TGTTGGTGGT CAGGACAAGA 
TCAGGCCCCT GTGGAGGCAC TATTTCCAGA ACACCCAGGG CCTCATTTTT 
GTTGTGGACA GCAATGACAG AGAGCGTGTT GTTGAGGCCA GGGATGAGCT 
CCACCGTATG CTGAATGAGG ATGAGCTACG TGATGCTGTG CTGCTGGTGT 
TTGCAAACAA ACAAGATCTT CCTAATGCCA TGAACGCTGC TGAGATCACC 
GACAAGCTTG GTCTGCACTC CTTGCGCCAG CGGCACTGGT ACATCCAGAG 
CACATGTGCT ACCTCTGGTG AGGGGTTGTA TGAGGGGCTT GACTGGCTTT 
CCAACAACAT TGCCAACAAG GCTTGAAGCA TCTTGGCCGG GCTTAAATGA 
AAGGAAGACA TGGCGGATAT CCCTGAGTGA ACACTGCCTT TTTACGTATC 
GAAACTATAT AGTCATGACA GATAATGATG TGGATGTTTT GGGAAGATAA 
AGCTCTTTAG GTTCCACCAC CAAAGGAGCT AGCTATCAGA TTGTTTTTCG 
TGGGGTGTTA AAAAATCGTG CGTGTTTCAG TTTGGTTTTT AATCGAAAGG 
CTTACGGTGC CTGACTTTTG TACCTCTGTA CTCGCCACAG ATGTTGTAGT 
ATTGACTTGT CTTGGGCATA GCGTATTGTT ACGTAATCCT GTTCTGTGCA 
GCCACCCCCA ATATCCTTAC CAGTTTATCC CCCGTTGCCT TCCTGGCCAG 
CATTCTGCTG CCTTTCTTCT TGGATGGCAC TTCATTCCTT TTCAGTAGCT 
TTCCGTATGT TTATATTGAA GTAAGCGCGT TGTAACGTCA GCCTGAAAAT 
TGCAAACCGA TCATTTCCTG TGGGCTGTAA GGGTTTCTTG GAACATAAGA 
ATTTTACAGG AATGGGTTTT GATCTCAATG AAATCCGTTT GGGC

Gene models with high sequence identity

AT3G62290.3 [+] show detail - ADP-ribosylation factor A1E
E-value: 2e-99; Score: 359.00

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Small GTP-binding protein	2.8e-18
HMMPfam	ARF/SAR superfamily	9.2e-80
superfamily	P-loop containing nucleoside triphosphate hyd	8.9e-56
ProfileScan	ARF	25.781
HMMSmart	ADP-ribosylation factor	3.8e-136
HMMSmart	Small GTPase SAR1-type	2.6e-26
HMMSmart	Ras small GTPase, Rab type	0.0032
HMMPanther	ADP-RIBOSYLATION FACTOR, ARF	4.1e-146
HMMPanther	ARF/SAR superfamily	4.1e-146
FPrintScan	ARF/SAR superfamily	1.3e-19
FPrintScan	ARF/SAR superfamily	1.3e-19
FPrintScan	ARF/SAR superfamily	1.3e-19
FPrintScan	ARF/SAR superfamily	1.3e-19
Gene3D	no description	5e-67

See the detailed result >

AT3G62290.3

Model type: Protein coding
Short Description: ADP-ribosylation factor A1E
Curator Summary: A member of ARF GTPase family. A thaliana has 21 members of this family, known to be essential for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division, cell expansion and cellulose production using antisense construct.
Computational Description: ADP-ribosylation factor A1E (ARFA1E); CONTAINS InterPro DOMAIN/s: ADP-ribosylation factor (InterPro:IPR006688), Small GTP-binding protein (InterPro:IPR005225), Ras small GTPase, Rab type (InterPro:IPR003579), Small GTPase SAR1-type (InterPro:IPR006687), ARF/SAR superfamily (InterPro:IPR006689); BEST Arabidopsis thaliana protein match is: Ras-related small GTP-binding family protein (TAIR:AT2G47170.1).
Link: InterPro Scan - TAIR