K21205

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K21205

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21205

Determination of introduced cDNA(s)

  • J033035C14 - Rice Full-length cDNAs - Original: J033035C14

    full reading [+] show sequence

    Nucleotide sequence: J033035C14 - full reading 
    GACACAACTC AGCTTGTATC TGAGACACGC AATGGCAAAG CATTCCACCA 
    CAATGAGCTG CCTCCTATTC TTCGTCTTGC TCTGTTTGGG CTCTCACTTA 
    GCCCAGGCTC AGGTTCTCTT TCAGGGATTC AACTGGGAGT CATGGAAGAA 
    GCAGGGTGGG TGGTACAACT TCCTCCATAG CCACGTCGAC GACATCGCCG 
    CGACCGGAGT CACACACGTC TGGCTCTCGC CGCCGTCGCA CTCCGTCGCG 
    CCACAGGGAT ACATGCCGGG ACGGCTCTAC GACCTGGACG CGTCCAAGTA 
    CGGGACGGGG GCAGAGCTCA GGTCGCTGAT CGCCGCCTTC CACAGCAAGA 
    GCATCAAGTG CGTCGCCGAC ATCGTCATCA ACCACCGGTG CGCGGATTAC 
    AAGGATAGCC GTGGCATCTA CTGCATCTTC GAGGGTGGCA CGCCGGACAG 
    CCGCCTCGAC TGGGGTCCCG ACATGATCTG CAGCGACGAC ACGCAGTACT 
    CCAACGGCCG CGGTCACCGC GATACTGGCG CAGACTTCGG CGCGGCGCCC 
    GACATCGACC ACCTCAACAC GCGTGTGCAG ACAGAGCTCT CAGACTGGCT 
    CAACTGGCTC AAGTCCGACG TCGGCTTCGA CGGCTGGCGC CTCGACTTCG 
    CCAAGGGATA CTCGGCGACC GTCGCCAAGA CGTACGTGGA CAACACCGAC 
    CCGTCCTTCG TCGTCGCCGA GATATGGAGC AACATGCGTT ACGACGGCAA 
    CGGTGAGCCG TCGTGGAACC AGGACGGTGA CCGGCAGGAG TTGGTGAACT 
    GGGCGCAGGC CGTCGGTGGC CCTGCATCGG CGTTCGACTT CACGACCAAG 
    GGCGAGCTGC AGGCAGCGGT GCAAGGTGAG CTGTGGCGGA TGAAGGATGG 
    CAACGGCAAG GCGCCGGGGA TGATTGGCTG GCTGCCGGAG AAGGCTGTCA 
    CCTTCATCGA CAATCATGAC ACTGGCTCCA CACAGAACTC ATGGCCGTTC 
    CCCTCCGACA AGGTCATGCA GGGCTACGCC TACATCCTCA CACACCCTGG 
    AGTACCCTGC ATTTTCTACG ACCATGTGTT TGACTGGAAC TTGAAGCAGG 
    AGATCAGCAC ATTGGCCGCG GTGAGGTCAA GAAATGGAAT TCATCCTGGG 
    AGCAAGCTGA ACATCCTTGC TGCTGACGGA GATGTCTACG TCGCAATGAT 
    CGATGACAAG GTGATCACAA AGATTGGGAC TCGGTATGAT GTGGGCAACT 
    TGATCCCGTC AGACTTCCAT GTCGTTGCTC ACGGCAACAA TTACTGCGTC 
    TGGGAAAAGA GTGGTCTTAG AGTTCCAGCA GGGCGGCGCC ACTAGGCCAA 
    ATATTTCTTT CAGGACTATT TTGTGCCTGA AATAAGAAGT TTAAATGATG 
    TCTTAAATAA CAAGAAAGTG ATTGATTGAT TGT

    Gene models with high sequence identity

    1. AT4G25000.1 [+] show detail - alpha-amylase-like
      E-value: 3e-147; Score: 518.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Glycoside hydrolase, subgroup, catalytic core 2.9e-100
    HMMPIR Alpha-amylase, plant 0
    HMMSmart Glycosyl hydrolase, family 13, subfamily, catalytic domain 2.1e-60
    HMMSmart Alpha-amylase, C-terminal beta-sheet 6.1e-40
    HMMPanther ALPHA-AMYLASE 2.4e-206
    HMMPanther AMYLASE 2.4e-206
    superfamily Glycoside hydrolase, catalytic core 3.5e-83
    HMMPfam Glycosyl hydrolase, family 13, catalytic domain 3.2e-26
    HMMPfam Alpha-amylase, C-terminal beta-sheet 3.2e-23
    FPrintScan Glycoside hydrolase family 13 1.4e-06
    FPrintScan Glycoside hydrolase family 13 1.4e-06
    FPrintScan Glycoside hydrolase family 13 1.4e-06

    See the detailed result >

    AT4G25000.1

    Model type
    Protein coding
    Short Description
    alpha-amylase-like
    Curator Summary
    Predicted to be secreted protein based on signalP prediction. Involved in starch mobilization. Mutants are defective in alpha-amylase activity. (Note: AMY1 has been found in the literature to be referred to as AMY3, which is not to be confused with AMY3/At1g69830).
    Computational Description
    alpha-amylase-like (AMY1); FUNCTIONS IN: alpha-amylase activity; INVOLVED IN: response to gibberellin stimulus, response to abscisic acid stimulus; LOCATED IN: extracellular region, apoplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 13 (InterPro:IPR006046), Alpha-amylase, plant (InterPro:IPR013775), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047), Alpha-amylase, C-terminal beta-sheet (InterPro:IPR012850); BEST Arabidopsis thaliana protein match is: alpha-amylase-like 2 (TAIR:AT1G76130.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR