K21317

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K21317

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K21317

Determination of introduced cDNA(s)

  • J033058D07 - Rice Full-length cDNAs - Original: J033058D07

    full reading [+] show sequence

    Nucleotide sequence: J033058D07 - full reading 
    GCAGCACAAG CAAGCAGCTT GAAGCAACCC ACCACAGCTT CCTTCTTCCT 
    CTGCTTCAGC AGAGCAGGGG AGCAGAGGAG GGAGAGGTCA CTCATTCACA 
    ACCTCATACA GAAGGAAGAA GAAGAAGAAG AGAAGAAGAT TCACACATAC 
    ATCTATAGAT AAAATATATT TATACATATA GAGAATACTC AGAGAGTAGC 
    CAAGAGAGGA GGAAGAAGAA GATGGTGTTG GTGGCGATTG GGGTGGTTGT 
    GGCGGCGGCG GTGGTGGTGA GCAGCCTGCT GCTGCGGTGG AACGAGGTGC 
    GGTACAGCCG GAAGCGCGGC CTGCCGCCGG GGACAATGGG GTGGCCGCTC 
    TTCGGCGAGA CCACCGAGTT CCTCAAGCAG GGACCCAGTT TCATGAAGGC 
    CCGGAGGCTC AGGTACGGGA GCGTGTTCAG GACGCACATC CTGGGGTGCC 
    CGACGGTGGT GTGTATGGAG GCGGAGCTGA ACCGGCGGGC GCTGGCCAGC 
    GAAGGGCGCG GGTTCGTCCC GGGCTACCCG CAGTCGATGC TGGACATCCT 
    GGGGCGGAAC AACATCGCCG CCGTGCAGGG CCCCCTCCAC CGCGCCATGC 
    GCGGCACCAT GCTCTCCCTC GTCCGCCCCG CCATGATCCG CTCCTCCCTC 
    CTCCCCAAGA TCGACGCCTT CATGCGCTCC CACCTCGCCG CCTGGTCCTC 
    CTCCTCCTCC TCCGCCGTCG TCGACATCCA GGCCAAGACC AAGGAGATGG 
    CCTTGCTATC TGCACTCAGG CAGATTGCCG GCGTCTCCGC TGGCCCACTC 
    TCTGACGCTC TCAAGGCAGA GCTCTACACC CTTGTGCTTG GCACCATCTC 
    CCTGCCCATC AACCTTCCTG GAACCAACTA CTACCAAGGC TTCAAGGCAA 
    GGAAGAAGCT TGTTGCAATG CTAGAGCAGA TGATCGCGGA ACGGCGATCC 
    TCCGGTCAGG TACACGACGA CATGCTGGAT GCGCTCTTGA CCGGTGTCGA 
    GGGCACCAGG GAGAAGCTCA CAGATGAGCA GATCATTGAC CTGATCATCA 
    CCCTTATATA CTCTGGATAT GAAACCATGT CGACGACCTC GATGATGGCT 
    GTCAAGTACC TGTCAGACCA TCCCAAAGCT CTTGAGCAAC TCAGGAAAGA 
    ACATTTTGAT ATCAGGAAAG GTAAAGCGCC CGAAGATGCC ATCGACTGGA 
    ATGATTTCAA GTCCATGACC TTCACTCGAG CTGTTATCTT CGAGACATTA 
    AGATTAGCTA CAGTTGTGAA TGGGCTGCTG AGGAAAACTA CCCAAGATGT 
    TGAAATGAAT GGGTATGTTA TCCCAAAAGG TTGGAGAATA TATGTTTACA 
    CAAGGGAAAT AAATTATGAT CCATTCCTGT ACCCTGATCC CATGACATTC 
    AATCCATGGA GGTGGCTGGA GAAGAACATG GAATCACATC CACACTTCAT 
    GCTGTTTGGA GGAGGTAGTC GAATGTGCCC GGGGAAGGAA GTAGGCACCG 
    TAGAAATTGC AACATTCCTT CACTATTTCG TGACTCAATA CAGATGGGAG 
    GAAGAAGGTA ACAACACAAT ATTGGAGTTC CCCCGAGTTG AAGCTCCCAA 
    CGGGTTACAT ATCCGCGTTC AAGATTACTG ATCCTGGAAC ATTTTGCGAA 
    GTTAGATAGG AATTCAAATC AGATAGAAAA TAAGAGTGCA ATTCTGATGC 
    TGCTGCCCTG ATGACAGGAT TAGAACAGCC GTTTGGCTCT TGTAACATAT 
    GTCCATGAAA ATAGGAATAT TATAGAGATA ATATAGGCAT TATAGGACCA 
    TTAGGAAGCT AATGTAGATT GCCATCAGAT GCTCCTGTAT TCTTTTGGTC 
    CATCAATTGT TGAACTAAAA CAGAAGTGTC ATTTGGCTTC TATGCTTCCG

    Gene models with high sequence identity

    1. AT3G30180.1 [+] show detail - brassinosteroid-6-oxidase 2
      E-value: 8e-164; Score: 574.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Cytochrome P450, conserved site 0
    Gene3D Cytochrome P450 4.7e-93
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, E-class, group IV 2.9e-15
    FPrintScan Cytochrome P450, C-terminal 8.2e-07
    FPrintScan Cytochrome P450, C-terminal 8.2e-07
    FPrintScan Cytochrome P450, C-terminal 8.2e-07
    FPrintScan Cytochrome P450, C-terminal 8.2e-07
    superfamily Cytochrome P450 2e-97
    HMMPfam Cytochrome P450 9.7e-63
    HMMPanther CYTOCHROME P450, SUBFAMILY 85 5e-247
    HMMPanther Cytochrome P450 5e-247

    See the detailed result >

    AT3G30180.1

    Model type
    Protein coding
    Short Description
    brassinosteroid-6-oxidase 2
    Curator Summary
    Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control.
    Computational Description
    brassinosteroid-6-oxidase 2 (BR6OX2); FUNCTIONS IN: monooxygenase activity, oxygen binding; INVOLVED IN: response to light stimulus, circadian rhythm, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: brassinosteroid-6-oxidase 1 (TAIR:AT5G38970.1); Has 29372 Blast hits to 29308 proteins in 1622 species: Archae - 48; Bacteria - 4489; Metazoa - 10449; Fungi - 5456; Plants - 7735; Viruses - 3; Other Eukaryotes - 1192 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR