K21715

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	decreased width (PATO:0000599) (Cauline leaf::Shape::narrow)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Shape::zigzag) present in greater numbers in organism (PATO:0000470) (Stem::Number::many) abnormal (PATO:0000460) (Stem::Others)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)

Invisible phenotypes

Hormones::Others::increase total Auxin

Original: K21715

Determination of introduced cDNA(s)

J023102D08 - Rice Full-length cDNAs - Original: J023102D08

full reading [+] show sequence

GATTCACGGG AGGTTGCCGT GTCGGGCGCG CGCGGGTGAT TGATTTCGCC 
GGCGGCGAGG GGAGGGAGGG AGGCAAAAGA GATGGACCGG GCGGCGCTGA 
CGGTGGGGCC GGGGATGGAC ATGCCGATAA TGCACGACGG CGACCGGTAC 
GAGCTGGTGC GGGACATCGG CTCCGGCAAC TTCGGCGTCG CGCGCCTCAT 
GCGCAGCCGC GCCGACGGCC AGCTCGTCGC CGTCAAGTAC ATCGAGCGCG 
GCGACAAGAT CGACGAGAAC GTGCAGCGGG AGATCATCAA CCACCGCTCG 
CTGCGCCACC CCAACATCAT CCGCTTCAAG GAGGTCATCC TCACCCCCAC 
CCACCTCGCC ATCGTCATGG AGTACGCCTC CGGCGGCGAG CTCTTCGAGC 
GCACCTGCAA CGCCGGCAGG TTCAGCGAGG ACGAGGCACG GTTCTTTTTC 
CAGCAACTGA TTTCAGGAGT CAGCTATTGC CATTCCATGC AAGTATGCCA 
TCGTGACCTG AAGCTGGAGA ACACCCTGCT CGACGGCAGC ACGGCGCCTC 
GCCTCAAGAT ATGCGACTTT GGCTATTCAA AGTCGTCTGT TCTTCATTCG 
CAACCAAAAT CTACTGTTGG AACTCCGGCA TACATCGCTC CTGAGGTTCT 
GCTGAAGAAG GAATATGATG GAAAGATTGC TGATGTGTGG TCGTGTGGAG 
TAACCCTCTA CGTAATGCTG GTTGGTGCAT ATCCTTTTGA GGATCCAGAT 
GAGCCTAAGA ATTTCAGGAA GACAATTCAG AGAATATTGG GTGTGCAGTA 
CTCTATTCCA GATTATGTCC ACATATCTCC AGAGTGCCGA GATCTTATTG 
CGAGGATTTT TGTGGCCAAC CCAGCCACTA GAATCTCTAT CCCCGAGATC 
AGAAATCATC CATGGTTCTT GAAGAATCTC CCAGCTGACC TTATGGATGA 
TAGCAAGATG AGCAGCCAGT ACGAGGAGCC CGAACAGCCA ATGCAGAGCA 
TGGATGAGAT CATGCAGATA CTGGCAGAGG CGACCATACC AGCAGCTGGG 
TCTGGTGGAA TCAACCAGTT CTTGAATGAT GGCCTTGACC TCGATGATGA 
CATGGAGGAC CTTGATTCAG ACCCCGATCT TGACGTGGAA AGCAGTGGGG 
AGATAGTATA CGCTATGTGA TGCTGTGAAT TCTAGCAAGC AGACAGGCAG 
ATTGCTTGTC AGTTGCCCGC GAATTGGTTT GTTGTATTAC AGTCACAGAT 
GACAAAGATG GCCAAGAAAC TGGGAAGTGG ATACATGTGT CAAGAGTACA 
TCACAAAAAA GTTCGGTTGC CTATGCTGTT TATTATGCCT GATGATCCCT 
GTTGTCTCCT GTTTTGTCGC CGTTGATTTG TGTATTGGAG TGTCCTAGAT 
CTCGTTTTGA GAACTGTTTG GAACCTTGTC TTTGCTCCCC TTAACTGAGG 
GAAAGCGATG TAGTGGTAGT AGTAGTAGTA GTAGCAGCGT GAAGACTGAA 
TAAGAGATGC CCCCAGTAAG CTCAGTACAC GAACTTGTTT CCATTTTCAA 
AGCACCATCT GCACTGCACG TCATTAATTT TATTTATGTT ACTTC

Gene models with high sequence identity

AT4G33950.1 [+] show detail - Protein kinase superfamily protein
E-value: 7e-166; Score: 580.00

InterPro Scan Digest

Program	Description	E-value
PatternScan	Protein kinase, ATP binding site	0
PatternScan	Serine/threonine-protein kinase, active site	0
HMMSmart	Serine/threonine-protein kinase domain	6.5e-98
HMMSmart	Tyrosine-protein kinase, catalytic domain	0.000000000003
Gene3D	no description	1.1e-60
superfamily	Protein kinase-like domain	1e-82
HMMPfam	Serine/threonine-protein kinase-like domain	1e-70
HMMPanther	Serine/threonine-protein kinase-like, plant	2.4e-200
HMMPanther	Calcium/calmodulin-dependent protein kinase-like	2.4e-200
ProfileScan	Protein kinase, catalytic domain	48.357

See the detailed result >

AT4G33950.1

Model type: Protein coding
Short Description: Protein kinase superfamily protein
Curator Summary: Encodes calcium-independent ABA-activated protein kinase, a member of SNF1-related protein kinases (SnRK2) whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Mutations disrupted ABA induction of stomatal closure as well as ABA inhibition of light-induced stomatal opening. However, regulation of stomatal opening/closing by light or CO(2) is not affected in these mutants. May act in the interval between ABA perception and reactive oxygen species production in the ABA signalling network.
Computational Description: OPEN STOMATA 1 (OST1); FUNCTIONS IN: calcium-dependent protein serine/threonine kinase activity, protein kinase activity, kinase activity; INVOLVED IN: in 14 processes; LOCATED IN: cytosol, nucleus, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636), Serine/threonine-protein kinase-like, plant (InterPro:IPR015740); BEST Arabidopsis thaliana protein match is: sucrose nonfermenting 1(SNF1)-related protein kinase 2.3 (TAIR:AT5G66880.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR